Search results for : drug discobery

This paper highlight presents a microfluidic approach with Fluigent’s Flow EZ, which can be used to create small solid lipid nanoparticles with high encapsulation rates for biologics and drugs.

In this application note, the Raydrop (developed and manufactured by Secoya) is used to perform Liposome Nanoparticle synthesis. 

We discuss some applications where microfluidics achieves results that would be very challenging to obtain when using conventional methods.

Many national and internationally funded projects bring together science and innovation. These projects cover a large number of subjects. Fluigent takes part in several European and French funded programs to provide expertise and resources in microfluidics and related applications.

This comprehensive guide will cover several essential definitions, advantages, and disadvantages of different technologies, considerations for choosing the right technology, and examples of OOC using Fluigent devices.

In this Application Note, PLGA nanoparticles with high monodispersity are generated using the Raydrop single emulsion developed and manufactured by Secoya, and Fluigent pressure-based flow controllers. The ability to synthesise PLGA nanoparticles in a more controllable and reproducible way creates possibilities to tailor surface properties and increase fields of application.

This application note describes the method for the production of double agarose emulsions using the RayDrop double emulsion chip and Fluigent pressure-based flow controllers. Agarose microcapsules are generated with precise control of particle, core and shell size.

PLGA microparticles are of great interest for life science applications. This application note focus on microfluidic PLGA microparticles generation methods.

Microfluidic systems are being developed as it is perfectly suited to making miniaturized, robust and controllable systems involving fluid handling and biological functions, such as human liver-on-a-chip models for instance. Use of Fluigent products for characterizing the microfluidic culture of liver tissue.

Engineered Nanoparticles (NPs) are becoming essential to enhance drug delivery systems (DDSs) for both biomedical and pharmaceutical applications. Controlling the delivery of molecules of interest, increases their concentration in a desired location and lead to improved drug efficacy as well as minor adverse effects. NPs including lipid-based and polymeric NPs have been used over the years as nanocarriers of various bioactive molecules.

Development of a cutting-edge technology that utilizes a state-of-the-art microfluidic system in the context of a novel tissue chip platform.

In this Application Note, PLGA shell/aqueous core microcapsules are obtained using the Secoya Raydrop Double emulsion. The influence of the fluidic parameters on the microcapsule size and release from the oil across the shell are explored in this application note.

Here we present here the Lego CE system consisting of available ready-to-use electrophoretic and microfluidic modules, including pressure-based flow controllers. The instrument is coupled with a laser-induced fluorescence detector (LIF) and is demonstrated for separations of labeled oligosaccharides

In this review, we propose an overview of the different techniques used to produce microsized aqueous compartments confined by a single lipid bilayer that separates them from the surrounding aqueous environment. 

Following is a method for encapsulation of reagent into alginate microbeads with total control of bead formation. A droplet-based microfluidic method is used to precisely control the production of microbeads without the drawbacks of large size distribution that present other methods.

Discover the concepts of OOC engineering and their applications in medical sciences.

The choice of the right method of fluid management can often determinate the success of a project, as well as the overall time and cost of manufacturing. Here is what you need to consider and be aware of when integrating fluidic control into your system.

In this short note, we present a Fluigent microfluidic solution for polymer microparticle synthesis with high monodispersity (2% CV). The microfluidic system allows for inline particle generation, spacing, and polymerization by UV light.

Droplet microfluidics is a powerful tool which consists of generating and manipulating micron-sized monodispersed droplets.

Microfluidics is both the science which studies the behaviour of fluids through micro-channels, and the technology of systems that process or manipulate small (10-6 to 10-12 litres) amounts of fluids using microminiaturized devices 

In this application note, we describe the process of producing highly monodisperse alginate microparticles using droplet-based microfluidic methods to control droplet size and core-shell ratio. Different concentrations of alginate have been used to show the versatility and adaptability of the RayDrop in multiple applications.

In this Application Note, chitosan-shell/oily-core microcapsules are generated using the Raydrop double emulsion chip, and Fluigent pressure-based flow controllers. The influence of the fluidic parameters on the size and the release from the oil across the shell are studied and presented.

Microfluidic devices are used for Bubble generation at micron-scale, yielding high monodispersity and well-controlled size. We investigated how parameters such as the geometry of the nozzle and the continuous-phase flow rate affects the microbubble formation process.

Our gut-on-chip system made of BEOnChip microfluidic chips coupled to pressure controllers offers a way to control and mimic the microenvironment of in vitro cultured intestinal epithelium that is closer to the physiological state.

In this application note, we describe the encapsulation of multiple aqueous “core” droplets inside a single oil droplet called a “shell”.

Cells are constantly exposed to biochemical stimulation from the early embryonic stage to adult life. The spatiotemporal regulation of these signals is essential as it determines cell fate, phenotype, metabolic activity as well as pathological behaviors. The fast response and high stability of Fluigent instruments make them the best solution available on the market to reproduce these complex variations in vitro.

Complete and in-depth overview on droplet microfluidics.

Microfluidics and lab-on-a-chip technology have emerged as the most promising approaches to address these challenges. These innovative technologies hold the potential to unlock new insights into single cell properties and their roles within populations.

This application presents the Liquid-phase Transmission Electron Microscopy (LPTEM) technique, which integrates integrates liquid flow capabilities within microfabricated liquid cells, providing the means to study different processes in solution with sub-nanometer spatial resolution and sub-microsecond temporal resolution. Using this technique, it is now possible to visually study topics ranging from material science to life science.

This case study highlights Rakesh et al.’s successful application of complex emulsions to advance real-time flow sensing technology.

Benoit Scheid, Senior Researcher, FNRS (National Foundation for Scientific Research).

Professor at the “Université Libre de Bruxelles”, teaching microfluidics to bioengineers and biomedical engineers.

Research activities focusing on process-orientated microfluidics.

Chairman of the Secoya board and scientific advisor.

Microfluidics is the science of manipulating and controlling fluids, usually in the range of microliters (10-6) to picoliters (10-12), in networks of channels with dimensions from tens to hundreds of micrometers. 

Complete and in-depth overview on production Complete and in-deph overview on microcapsule production.

This paper assesses a new temperature measurement method at the micro-scale at high throughput.

CloneSeq: A highly sensitive single-cell analysis platform for the comprehensive characterization of cells from 3D culture.

Myochip: Building a 3D innervated and irrigated muscle on a chip

The aim of the European Union’s Horizon H2020 project, Myochip  is to build a muscle on chip. Myochip gathers 4 partners from all around the world: The institute of molecular medicine (Portugal), The University of Edinburg (Scotland), and Institut Curie (France).

In this application note we have demonstrated how we can perform different type of double emulsion generation with a single device.

In this application note, we present droplet generation data obtained using one of the most widely used water in oil emulsion systems – water in decane. We demonstrate the ability of Fluigent equipment coupled with RayDrop microfluidic devices to generate high-quality emulsions with controlled droplet sizes and with high throughput.

This paper highlight evaluates the flow uncertainty for Fluigent MSFC-EZ and syringe pumps and their impact on optical imaging.

Microbead-based microfluidics is a powerful technique that generates highly monodispersed picoliter-sized beads into a continuous phase. This method has been successfully adapted to cell culture to encapsulate cells in micron size hydrogel beads. The main advantages are reduced costs related to miniaturization, high reproducibility, and high throughput screening capacities.

Microfluidic chips, often referred to as lab-on-a-chip devices, are miniature platforms that manipulate and analyze small volumes of fluids. We here define microfluidic chips and summarize microfluidic history.

Flow rate measurement is needed for many microfluidic applications, including microfluidic droplet generation, cell culture under flow, and organs on chip. Several technologies aim to provide accurate real time flow measurement to meet this need. In this review, the different existing microfluidic flow sensing solutions for low-flow liquids are described, with their advantages and current limits, as progress is still ongoing in this field.

We have demonstrated the use of Aria and its related software for the automated delivery of different liquids for the capture and labeling of cancer cells (breast cancer) using a complex microfluidic setup.

In this short blog, we review the most important aspects to consider when selecting fluid handling OEM components.

Our advanced platform for long-term recirculation of fluid, ideal for Organ-on-a-Chip studies and the reproduction of relevant physiological conditions, providing deeper insights into in-vivo phenomena.