Search results for : drug discobery
Solid lipid nanoparticles for biologics and drug encapsulation
This paper highlight presents a microfluidic approach with Fluigent’s Flow EZ, which can be used to create small solid lipid nanoparticles with high encapsulation rates for biologics and drugs.
Microfluidic Drug Discovery
Microfluidic automated fluidic workflows as valuable tools for drug discovery
Liposome Nanoparticle Synthesis
In this application note, the Raydrop (developed and manufactured by Secoya) is used to perform Liposome Nanoparticle synthesis.
Microfluidic chips: key applications
We discuss some applications where microfluidics achieves results that would be very challenging to obtain when using conventional methods.
Funded research program participation
Many national and internationally funded projects bring together science and innovation. These projects cover a large number of subjects. Fluigent takes part in several European and French funded programs to provide expertise and resources in microfluidics and related applications.
Microfluidic pressure control for organ-on-a-chip applications: A comprehensive guide
This comprehensive guide will cover several essential definitions, advantages, and disadvantages of different technologies, considerations for choosing the right technology, and examples of OOC using Fluigent devices.
PLGA nanoparticle synthesis using 3D microfluidic hydrodynamic focusing
In this Application Note, PLGA nanoparticles with high monodispersity are generated using the Raydrop single emulsion developed and manufactured by Secoya, and Fluigent pressure-based flow controllers. The ability to synthesise PLGA nanoparticles in a more controllable and reproducible way creates possibilities to tailor surface properties and increase fields of application.
Agarose Microcapsules Synthesis
This application note describes the method for the production of double agarose emulsions using the RayDrop double emulsion chip and Fluigent pressure-based flow controllers. Agarose microcapsules are generated with precise control of particle, core and shell size.
Drug encapsulation in biocompatible microparticles for drug deliveryRead more
Flow Expertise for Cell Encapsulation and Single-Cell Analysis
The ability to precisely control the flow rate allows to regulate the size of the droplet & their production frequency & the cell encapsulation efficiency.
Microfluidics for Droplet Generation
One of the best-known research topics in microfluidics with a diverse range of applications is the generation of highly-monodisperse droplets. Through the use of channel geometry, immiscible fluid properties, and precise flow control, single or double droplets with diameters from 10 to a few hundred micrometers can be produced at high throughput rates (up to 10kHz). In the last decade, monodisperse droplet production has drawn considerable interest in the scientific community, with a variety of applications in single-cell analysis, drug delivery, sequencing, and other fields of research.
PLGA Microparticles Synthesis
PLGA are of for life science applications. This application note focus on PLGA .
Microfluidics for Organ-on-chip Cell culture
The concept of this microfluidic research application is to reproduce organ functions (lungs, gut, cartilage, heart, etc.) within a cell culture incubated inside a microfluidic chip. The ability of microfluidics to mimic the in-vivo cellular microenvironment by reproducing blood or interstitial fluid flows to provide cells with a nutrient supply and mechanical constraints makes it the ideal tool for this application. Little by little, organ-on-a-chip technologies are overtaking conventional techniques, which are less physiologically relevant.
CNRS/UTC: study of a liver-on-a-chip model
Microfluidic systems are being developed as it is perfectly suited to making miniaturized, robust and controllable systems involving fluid handling and biological functions, such as human liver-on-a-chip models for instance. Use of Fluigent products for characterizing the microfluidic culture of liver tissue.
Microfluidics Technology for the Design and Formulation of Nanoparticles
Engineered Nanoparticles (NPs) are becoming essential to enhance drug delivery systems (DDSs) for both biomedical and pharmaceutical applications. Controlling the delivery of molecules of interest, increases their concentration in a desired location and lead to improved drug efficacy as well as minor adverse effects. NPs including lipid-based and polymeric NPs have been used over the years as nanocarriers of various bioactive molecules.
Microfluidics for Cell Biology
Cell biology is at the heart of medical science. Researchers seek to understand how cells organize to form human tissue, how they communicate with each other, how they respond to infections or other pathologies, and how we can help them to fight such pathologies with drugs or other treatments. Microfluidics brings applications of this research to a higher level with reduced costs, faster experiments, enhanced detection thresholds, and strong multiplexing potential.
University of Rochester: A tissue chip platform for real-time sensing of secreted inflammatory markers using ARIA
Development of a cutting-edge technology that utilizes a state-of-the-art microfluidic system in the context of a novel tissue chip platform.
PLGA microcapsules synthesis
In this Application Note, PLGA shell/aqueous core microcapsules are obtained using the Secoya Raydrop Double emulsion. The influence of the fluidic parameters on the microcapsule size and release from the oil across the shell are explored in this application note.
Capillary electrophoresis using microfluidic, electrophoretic, and optic modules
Here we present here the Lego CE system consisting of available ready-to-use electrophoretic and microfluidic modules, including pressure-based flow controllers. The instrument is coupled with a laser-induced fluorescence detector (LIF) and is demonstrated for separations of labeled oligosaccharides
Giant Unilamellar Vesicles (GUVs) Production using Microfluidics
In this review, we propose an overview of the different techniques used to produce microsized aqueous compartments confined by a single lipid bilayer that separates them from the surrounding aqueous environment.
Alginate Microbeads Production
Following is a method for encapsulation of reagent into alginate microbeads with total control of bead formation. A droplet-based microfluidic method is used to precisely control the production of microbeads without the drawbacks of large size distribution that present other methods.
Microfluidic white paper - A review of Organ on Chip Technology
Discover the concepts of OOC engineering and their applications in medical sciences.
University of Cambridge: Giant unilamellar vesicle production and testingRead more
Key considerations for fluidic system integration
The choice of the right method of fluid management can often determinate the success of a project, as well as the overall time and cost of manufacturing. Here is what you need to consider and be aware of when integrating fluidic control into your system.
UV-Crosslinking of Microparticles
In this short note, we present a Fluigent microfluidic solution for polymer microparticle synthesis with high monodispersity (2% CV). The microfluidic system allows for inline particle generation, spacing, and polymerization by UV light.
Microfluidic Droplet Production Method
Droplet microfluidics is a powerful tool which consists of generating and manipulating micron-sized monodispersed droplets.
Microfluidics for Cell Analysis
Microfluidics offers innovative ways of studying cellular behaviors. Its ability to control flow in a precise manner offers new ways of studying cell interactions with drugs, other cells or bacteria in single-cell conditions, as well as cell response to a mechanical stress or even efficient sorting of cells from complex cellular populations using fluorescence-based or biomechanics-based methods.
What is the history of microfluidics?
Microfluidics is both the science which studies the behaviour of fluids through micro-channels, and the technology of systems that process or manipulate small (10-6 to 10-12 litres) amounts of fluids using microminiaturized devices
Alginate Microcapsule Synthesis
In this application note, we describe the process of producing highly monodisperse alginate microparticles using droplet-based microfluidic methods to control droplet size and core-shell ratio. Different concentrations of alginate have been used to show the versatility and adaptability of the RayDrop in multiple applications.
Microfluidic Chitosan Microcapsules Production
In this Application Note, chitosan-shell/oily-core microcapsules are generated using the Raydrop double emulsion chip, and Fluigent pressure-based flow controllers. The influence of the fluidic parameters on the size and the release from the oil across the shell are studied and presented.
Microbubble formation using the RayDrop
Microfluidic devices are used for Bubble generation at micron-scale, yielding high monodispersity and well-controlled size. We investigated how parameters such as the geometry of the nozzle and the continuous-phase flow rate affects the microbubble formation process.
Development of a human gut-on-chip to assess the effect of shear stress on intestinal functions
Our gut-on-chip system made of BEOnChip microfluidic chips coupled to pressure controllers offers a way to control and mimic the microenvironment of in vitro cultured intestinal epithelium that is closer to the physiological state.
Encapsulation of multiple emulsions in a single droplet
In this application note, we describe the encapsulation of multiple aqueous “core” droplets inside a single oil droplet called a “shell”.
Mimicking in-vivo environments: biochemical and biomechanical stimulation
Cells are constantly exposed to biochemical stimulation from the early embryonic stage to adult life. The spatiotemporal regulation of these signals is essential as it determines cell fate, phenotype, metabolic activity as well as pathological behaviors. The fast response and high stability of Fluigent instruments make them the best solution available on the market to reproduce these complex variations in vitro.
Complete and in-depth overview on droplet microfluidics.
High Throughput Single Cell Analysis
Microfluidics and lab-on-a-chip technology have emerged as the most promising approaches to address these challenges. These innovative technologies hold the potential to unlock new insights into single cell properties and their roles within populations.
Microfluidics for Transmission Electron Microscopy: Characterization of Copper Electrodeposition
This application presents the Liquid-phase Transmission Electron Microscopy (LPTEM) technique, which integrates integrates liquid flow capabilities within microfabricated liquid cells, providing the means to study different processes in solution with sub-nanometer spatial resolution and sub-microsecond temporal resolution. Using this technique, it is now possible to visually study topics ranging from material science to life science.
Complex Emulsions as Real-Time Flow Sensor
This case study highlights Rakesh et al.’s successful application of complex emulsions to advance real-time flow sensing technology.
Interview with Benoit Scheid from Secoya
Benoit Scheid, Senior Researcher, FNRS (National Foundation for Scientific Research).
Professor at the “Université Libre de Bruxelles”, teaching microfluidics to bioengineers and biomedical engineers.
Research activities focusing on process-orientated microfluidics.
Chairman of the Secoya board and scientific advisor.
Microfluidics definitions and advantages
Microfluidics is the science of manipulating and controlling fluids, usually in the range of microliters (10-6) to picoliters (10-12), in networks of channels with dimensions from tens to hundreds of micrometers.
Double emulsion for the generation of microcapsules - a Review
Complete and in-depth overview on production Complete and in-deph overview on microcapsule production.
Using dSurf for High Throughput Laser-Induced Fluorescence Droplet Micro-Thermometry (LuMIn)
This paper assesses a new temperature measurement method at the micro-scale at high throughput.
The Hebrew University: Encapsulation and culture in 3D hydrogels for Single cell sequencing
: A sensitive single- platform for the of 3D culture.
MYOCHIP | H2020 European project
Myochip: Building a 3D innervated and irrigated muscle on a chip
The aim of the European Union’s Horizon H2020 project, Myochip is to build a muscle on chip. Myochip gathers 4 partners from all around the world: The institute of molecular medicine (Portugal), The University of Edinburg (Scotland), and Institut Curie (France).
Double Emulsion Generation
In this application note we have demonstrated how we can perform different type of double emulsion generation with a single device.
Water in Oil Emulsions
In this application note, we present droplet generation data obtained using one of the most widely used water in oil emulsion systems – water in decane. We demonstrate the ability of Fluigent equipment coupled with RayDrop microfluidic devices to generate high-quality emulsions with controlled droplet sizes and with high throughput.
Pressure-driven flow controllers vs. Syringe pumps: A flow precision evaluation for optical blood imaging.
This paper highlight evaluates the flow uncertainty for Fluigent MSFC-EZ and syringe pumps and their impact on optical imaging.
Prostate Organoid Culture in Microbeads
Microbead-based microfluidics is a powerful technique that generates highly monodispersed picoliter-sized beads into a continuous phase. This method has been successfully adapted to cell culture to encapsulate cells in micron size hydrogel beads. The main advantages are reduced costs related to miniaturization, high reproducibility, and high throughput screening capacities.
Webinar - Polymerization of microfluidics-produced liquid crystal double emulsions for making wavelength and polarization-selective retroreflectorsRead more
Mastering Microfluidic Chips: An In-Depth Definition
Microfluidic chips, often referred to as lab-on-a-chip devices, are miniature platforms that manipulate and analyze small volumes of fluids. We here define microfluidic chips and summarize microfluidic history.
Microfluidic Flow Sensing Technologies, A Review
Flow rate measurement is needed for many microfluidic applications, including microfluidic droplet generation, cell culture under flow, and organs on chip. Several technologies aim to provide accurate real time flow measurement to meet this need. In this review, the different existing microfluidic flow sensing solutions for low-flow liquids are described, with their advantages and current limits, as progress is still ongoing in this field.
Cancer Cell Analysis Made Easy with Aria: cell Capture and Labeling
We have demonstrated the use of Aria and its related software for the automated delivery of different liquids for the capture and labeling of cancer cells (breast cancer) using a complex microfluidic setup.
Key reliability indicators for OEM components to ensure long-term performance of your flow control system
In this short blog, we review the most important aspects to consider when selecting fluid handling OEM components.
Long-term fluid recirculation system for Organ-on-a-Chip applications
Our advanced platform for long-term recirculation of fluid, ideal for Organ-on-a-Chip studies and the reproduction of relevant physiological conditions, providing deeper insights into in-vivo phenomena.
Flow gradient chip for 3D cell cultures
Chip for electrochemical gradients to 3D cell cultures
PLGA Microparticle Production Standard Pack
PLGA Microparticle Production Pack (Standard Pack)
Digital High-speed Microscope Camera
A dedicated microscope for microfluidic experiments
PLGA Microparticle Production Pack (Automation Pack)
PLGA Microparticle Production Pack (Automation Pack)
Rotary multi-port microfluidic valve for industry
OEM microfluidic electric rotary valve with multi-port