Microfluidic Application Notes
1-10 microns PLGA microsphere production using the RayDrop
We demonstrate the RayDrop’s ability to produce PLGA microparticles in the range of 1 to 10 µm, for encapsulation and drug delivery.
Automating Neuronal Cell Immunofluorescence in Microfluidic Chips
Streamline immunolabeling of neuron cells across as many as 4 microfluidic chips with an automated sequential injection system.
Pressure predictions for lab-on-chip operations using a microfluidic network solver and Fluigent PX
In this application note, we report on the combination of Fluigent PX with a microfluidic network solver for highly accurate predicted pressures and millisecond response times.
A quick and efficient double encapsulation method for FACS-based droplet sorting
In this application note, we associate the double emulsion microfluidic platform with FACS sorting technology to sort out small droplets encapsulating fluorescent e-coli bacteria.
Long-term fluid recirculation system for Organ-on-a-Chip applications
Our advanced platform for long-term recirculation of fluid, ideal for Organ-on-a-Chip studies and the reproduction of relevant physiological conditions, providing deeper insights into in-vivo phenomena.
Automated Immunofluorescence using Aria
Cutting-edge Automated Immunofluorescence protocol that leverages the capabilities of the automated sequential injection system
What is the best method for Microencapsulation of Bacteria and Yeast in Small Double Emulsions?
Encapsulation method of the yeast strain and the bacterial strain in double emulsions
Encapsulation of Cells In Small Double Emulsions
In this application note, we have demonstrated that the Cell Encapsulation Platform can encapsulate cells in w/o/w double emulsions with precise droplet size control. Human Adult Peripheral Blood Mononuclear Cells (PBMC) were encapsulated in a 50 µm monodisperse water-oil-water double emulsion using a RaydropTM with a 60-120-60 configuration
Alginate Microcapsule Synthesis
In this application note, we describe the process of producing highly monodisperse alginate microparticles using droplet-based microfluidic methods to control droplet size and core-shell ratio. Different concentrations of alginate have been used to show the versatility and adaptability of the RayDrop in multiple applications.
Agarose Microcapsules Synthesis
This application note describes the method for the production of double agarose emulsions using the RayDrop double emulsion chip and Fluigent pressure-based flow controllers. Agarose microcapsules are generated with precise control of particle, core and shell size.
PLGA nanoparticle synthesis using 3D microfluidic hydrodynamic focusing
In this Application Note, PLGA nanoparticles with high monodispersity are generated using the Raydrop single emulsion developed and manufactured by Secoya, and Fluigent pressure-based flow controllers. The ability to synthesise PLGA nanoparticles in a more controllable and reproducible way creates possibilities to tailor surface properties and increase fields of application.
Encapsulation of multiple emulsions in a single droplet
In this application note, we describe the encapsulation of multiple aqueous “core” droplets inside a single oil droplet called a “shell”.
Microbubble formation using the RayDrop
Microfluidic devices are used for Bubble generation at micron-scale, yielding high monodispersity and well-controlled size. We investigated how parameters such as the geometry of the nozzle and the continuous-phase flow rate affects the microbubble formation process.
Development of a human gut-on-chip to assess the effect of shear stress on intestinal functions
Our gut-on-chip system made of BEOnChip microfluidic chips coupled to pressure controllers offers a way to control and mimic the microenvironment of in vitro cultured intestinal epithelium that is closer to the physiological state.
PLGA microcapsules synthesis
In this Application Note, PLGA shell/aqueous core microcapsules are obtained using the Secoya Raydrop Double emulsion. The influence of the fluidic parameters on the microcapsule size and release from the oil across the shell are explored in this application note.
Pressure predictions for lab-on-a-chip operations using a microfluidic network solver and Fluigent PXDownloadDirect download
Droplet and particle manipulation using electrophoretic flow control
Many microfluidic applications are developed based on concepts relying on electrodes, exploiting the electrical properties of samples to sort or separate them, generate electro-osmotic flows, manipulate particles, perform electrochemical detection etc. One example of this is electronic paper, where the electrophoretic displacement of titanium dioxide particles in a dark hydrocarbon oil solution allows the formation of a black and white pattern with a high resolution.
Liposome Nanoparticle Synthesis
In this application note, the Raydrop (developed and manufactured by Secoya) is used to perform Liposome Nanoparticle synthesis.
High-throughput cell DNA screening using digital PCR
Thanks to droplet microfluidic, Michael Ryckelynck and his team are able to isolate single DNA molecules and analyze the enzymes and proteins resulting from their expression.
Cartilage-on-a-chip, an example of complex mechanical stimulation using Fluigent’s technology
A new method for 3D chondrocyte culture using a microfluidic platform that allows researchers to create a relevant cartilage model, subject to complex mechanical stimulation.
PLGA Microparticles Synthesis
PLGA are of for life science applications. This application note focus on PLGA .
E. Coli Culture in Droplets Using dSURF Fluorosurfactant
In this application note, discover how using microfluidics allows one to encapsulate single or multiple cells into tiny droplets of pL volume which are generated at a rate of approximately one thousand per second.
Droplet Sequencing: Drop-Seq method
The Drop-Seq protocol, is a high throughput method that enables the sequencing of the mRNA from a large number of cells. With this method it is possible to create a gene expression map of the cell, or even distinguish cell populations within a tissue!
Single cell sorter microfluidic platform
Passive cell or particle sorting using a dedicated package. To demonstrate the separation of particle mixtures, a solution containing 7.5 µm and 15 µm diameter polystyrene particles labeled with FITC, and TRITC fluorophores respectively was used. The particle streams were viewed and captured separately using appropriate filter cubes.
Microbiome culture in droplet using dsurf surfactant
The performance of three commonly used surfactants are compared at three different concentrations. The droplet stability over time and the droplet occupation rate is determined by encapsulating a microbial community derived from human skin.
Microfluidic Chitosan Microcapsules Production
In this Application Note, chitosan-shell/oily-core microcapsules are generated using the Raydrop double emulsion chip, and Fluigent pressure-based flow controllers. The influence of the fluidic parameters on the size and the release from the oil across the shell are studied and presented.
Generating a water emulsion in an oil solution using a droplet generator chip
In this application note, We explain how to generate water emulsions in oil solutions using the Fluigent microfluidic system including pressure pumps, chemicals, tubing and a Droplet Generator PDMS Chip.
Alginate Microbeads Production
Following is a method for encapsulation of reagent into alginate microbeads with total control of bead formation. A droplet-based microfluidic method is used to precisely control the production of microbeads without the drawbacks of large size distribution that present other methods.
Double Emulsion Generation
In this application note we have demonstrated how we can perform different type of double emulsion generation with a single device.
Assess Cell Proliferation Using Pressure as a Tool
This application presents a simple method to monitor cell proliferation in microfluidic chips in real time. This is demonstrated experimentally using a custom microfluidic chip. Cell morphology was studied under flowing and static culture condition
Capillary electrophoresis using microfluidic, electrophoretic, and optic modules
Here we present here the Lego CE system consisting of available ready-to-use electrophoretic and microfluidic modules, including pressure-based flow controllers. The instrument is coupled with a laser-induced fluorescence detector (LIF) and is demonstrated for separations of labeled oligosaccharides
Microfluidics for Transmission Electron Microscopy: Characterization of Copper Electrodeposition
This application presents the Liquid-phase Transmission Electron Microscopy (LPTEM) technique, which integrates integrates liquid flow capabilities within microfabricated liquid cells, providing the means to study different processes in solution with sub-nanometer spatial resolution and sub-microsecond temporal resolution. Using this technique, it is now possible to visually study topics ranging from material science to life science.
Analysis of a commercial surfactant for digital PCR assay
In this application note we are investigating the usability of the commercially available surfactant dSurf for an exemplary digital PCR-assay.
UV-Crosslinking of Microparticles
In this short note, we present a Fluigent microfluidic solution for polymer microparticle synthesis with high monodispersity (2% CV). The microfluidic system allows for inline particle generation, spacing, and polymerization by UV light.
UV-Crosslinking of Microcapsules
In this application note, we describe the UV-crosslinking of microcapsules process, in which microcapsules are formed by consolidating shell phase of the resulting double emulsions by uv-crosslinking of monomers and photoinitiator used as shell phase.
Water in Fluorocarbon Oil Emulsions
Achieve reproducible, high-quality water in fluorocarbon oil emulsions with cutting-edge microfluidic devices. Ideal for Drop-Seq and RNA-Seq studies.
Water in Oil Emulsions
In this application note, we present droplet generation data obtained using one of the most widely used water in oil emulsion systems – water in decane. We demonstrate the ability of Fluigent equipment coupled with RayDrop microfluidic devices to generate high-quality emulsions with controlled droplet sizes and with high throughput.
Oil in Water Emulsions
In this application note, we present droplet generation data obtained using decane in Water, a system that demonstrates the expected behavior of most hydrocarbons in Water. We demonstrate the ability of Fluigent equipment coupled with Raydrop microfluidic devices to generate high-quality emulsions with controlled droplet sizes and with high throughput.
Impedance Measurement of Microbeads
We present in this application note our Electrical Impedance Spectroscopy Platform (or EISP) consisting of microfluidic flow controllers from Fluigent to maintain precise flow control, a chip from Micronit Microtechnologies B. V to localize impedance measurements, and a lock-in amplifier from Zurich Instruments to perform impedance measurements. We demonstrate the system efficiency by determining the size of micrometer beads and by measuring the generation rate water-in-oil droplets.
Peristaltic Pump vs Pressure-Based Microfluidic Flow Control for Organ on Chip applications
The importance of flow stability in microfluidic systems is demonstrated by performing cell culture using peristaltic pumps or pressure-based flow controllers.
Cancer Cell Analysis Made Easy with Aria: cell Capture and Labeling
We have demonstrated the use of Aria and its related software for the automated delivery of different liquids for the capture and labeling of cancer cells (breast cancer) using a complex microfluidic setup.