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Microfluidic Application Notes

Fluigent’s broad range of solutions for nanofluidic and microfluidic application note, both in scientific research and the industrial/OEM sector, offer greater control, automation, precision, and ease of use. We offer modern microfluidic systems and components that enhance productivity. Whether your application features droplets, cell biology, particle studies, or other research areas, we have the expertise and knowledge to provide the most cost effective and technically advanced solutions for your fluid control needs. We offer best-in-class standard and custom OEM components, system development, and engineering services along with strong expertise in fluid management to accelerate your development process. This page features numerous microfluidic application notes that can help you with your setup and experiments. You will find tutorials, accompanied by an extensive and varied literature search, explaining the step by step process of how to perform the experiment optimally and efficiently, as well as the results obtained and the conclusion drawn from the experiment. Download detailed tutorials on microfluidic applications in the industrial and research sectors and learn more about Fluigent’s product portfolio! Other applications may be developed with the product's brand provided by Fluigent. Have you published a paper using Fluigent products? Contact us at marketing@fluigent.com and we will reference it on our site!
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Microfluidic Application Notes

Pressure predictions for lab-on-chip operations using a microfluidic network solver and Fluigent PX 

In this application note, we report on the combination of Fluigent PX with a microfluidic network solver for highly accurate predicted pressures and millisecond response times. 

 

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droplet sorting and microscopic observation of E COLI ENCAPSULATION
Microfluidic Application Notes

A quick and efficient double encapsulation method for FACS-based droplet sorting

In this application note, we associate the double emulsion microfluidic platform with FACS sorting technology to sort out small droplets encapsulating fluorescent e-coli bacteria.

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fluid recirculation system, omi and chip
Microfluidic Application Notes

Long-term fluid recirculation system for Organ-on-a-Chip applications

Our advanced platform for long-term recirculation of fluid, ideal for Organ-on-a-Chip studies and the reproduction of relevant physiological conditions, providing deeper insights into in-vivo phenomena.

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Microfluidic Application Notes

Automated Immunofluorescence using Aria

Cutting-edge Automated Immunofluorescence protocol that leverages the capabilities of the automated sequential injection system

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Microfluidic Application Notes

What is the best method for Microencapsulation of Bacteria and Yeast in Small Double Emulsions?

Encapsulation method of the yeast strain and the bacterial strain in double emulsions

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Microfluidic Application Notes

Cell Encapsulation In Small Double Emulsions

In this application note, we have demonstrated that the Cell Encapsulation Platform can encapsulate cells in w/o/w double emulsions with precise droplet size control. Human Adult Peripheral Blood Mononuclear Cells (PBMC) were encapsulated in a 50 µm monodisperse water-oil-water double emulsion using a RaydropTM with a 60-120-60 configuration

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Alginate Microcapsule Synthesis
Microfluidic Application Notes

Alginate Microcapsule Synthesis

In this application note, we describe the process of producing highly monodisperse alginate microparticles using droplet-based microfluidic methods to control droplet size and core-shell ratio. Different concentrations of alginate have been used to show the versatility and adaptability of the RayDrop in multiple applications.

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Agarose microcapsules in suspension zoom
Microfluidic Application Notes

Agarose Microcapsules Synthesis

This application note describes the method for the production of double agarose emulsions using the RayDrop double emulsion chip and Fluigent pressure-based flow controllers. Agarose microcapsules are generated with precise control of particle, core and shell size.

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plga nanoparticle synthesis acetone and water pva
Microfluidic Application Notes

PLGA nanoparticle synthesis using 3D microfluidic hydrodynamic focusing

In this Application Note, PLGA nanoparticles with high monodispersity are generated using the Raydrop single emulsion developed and manufactured by Secoya, and Fluigent pressure-based flow controllers. The ability to synthesise PLGA nanoparticles in a more controllable and reproducible way creates possibilities to tailor surface properties and increase fields of application.

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Microfluidic Application Notes

Encapsulation of multiple emulsions in a single droplet 

In this application note, we describe the encapsulation of multiple aqueous “core” droplets inside a single oil droplet called a “shell”.

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nozzle for bubble generation
Microfluidic Application Notes

Microbubble formation using the RayDrop

Microfluidic devices are used for Bubble generation at micron-scale, yielding high monodispersity and well-controlled size. We investigated how parameters such as the geometry of the nozzle and the continuous-phase flow rate affects the microbubble formation process.

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gut-on-chip caco 2 monolayer
Microfluidic Application Notes

Development of a human gut-on-chip to assess the effect of shear stress on intestinal functions

Fluid flow and shear stress have previously been reported to promote proper intestinal cell differentiation, formation of villus-like 3D structures and enhanced intestinal barrier function.

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monodisperse plga double emulsion
Microfluidic Application Notes

PLGA microcapsules synthesis

In this Application Note, PLGA shell/aqueous core microcapsules are obtained using the Secoya Raydrop Double emulsion. The influence of the fluidic parameters on the microcapsule size and release from the oil across the shell are explored in this application note.

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Microfluidic Application Notes

Pressure predictions for lab-on-a-chip operations using a microfluidic network solver and Fluigent PX

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WaterInOil-schema
Microfluidic Application Notes

Droplet and particle manipulation using electrophoretic flow control

Many microfluidic applications are developed based on concepts relying on electrodes, exploiting the electrical properties of samples to sort or separate them, generate electro-osmotic flows, manipulate particles, perform electrochemical detection etc. One example of this is electronic paper, where the electrophoretic displacement of titanium dioxide particles in a dark hydrocarbon oil solution allows the formation of a black and white pattern with a high resolution.

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Microfluidic Application Notes

Liposome Nanoparticles Synthesis

Liposomes were discovered in the 1960s. These hollow nanoparticles are phospholipid vesicles consisting of at least one lipid bilayer. This bilayer is usually composed of amphiphilicphospholipids that have a hydrophilic phosphate head and a hydrophobic tail consisting of two fatty acid chains. This structural feature has facilitated liposomes’ applications, including their use as artificial cell membranes, carriers for drug delivery systems, encapsulating agents forfood ingredients, and analytical tools.

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High-throughput-cell-DNA-encapsulation-graph
Microfluidic Application Notes

High-throughput cell DNA screening using digital PCR

The use of water-in-oil droplets in microfluidics in high-throughput screening is rapidly gaining acceptance. The main application areas currently involve screening cells as well as genetic material for various mutations or activity. Thanks to droplet microfluidic, Michael Ryckelynck and his team are able to isolate single DNA molecules and analyze the enzymes and proteins resulting from their expression.

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Chondrocyte deformation
Microfluidic Application Notes

Cartilage-on-a-chip, an example of complex mechanical stimulation using Fluigent’s technology    

A new method for 3D chondrocyte culture using a microfluidic platform that allows researchers to create a relevant cartilage model, subject to complex mechanical stimulation.

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Microfluidic Application Notes

PLGA Microparticles Synthesis

When PLGA is used as an active pharmaceutical ingredient carrier it is important to produce highly monodispersed particles for drug release reproducibility. The most common production process of PLGA particles is solvent based and can involve hazardous solutions. Ethyl acetate is preferred as it shows better biocompatibility than other conventional solvent such as dichloromethane.

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dSurf-Droplet2
Microfluidic Application Notes

E. Coli Culture in Droplets Using dSURF Fluorosurfactant 

In this application note, discover how using microfluidics allows one to encapsulate single or multiple cells into tiny droplets of pL volume which are generated at a rate of approximately one thousand per second.

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dropseq method
Microfluidic Application Notes

Droplet Sequencing: Using droplet compartmentalization to improve Next-Generation Sequencing 

The Drop-Seq protocol, is a high throughput method that enables the sequencing of the mRNA from a large number of cells. With this method it is possible to create a gene expression map of the cell, or even distinguish cell populations within a tissue!

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microfluidic cell sorting
Microfluidic Application Notes

Single cell sorter microfluidic platform

Passive cell or particle sorting using a dedicated package. To demonstrate the separation of particle mixtures, a solution containing 7.5 µm and 15 µm diameter polystyrene particles labeled with FITC, and TRITC fluorophores respectively was used. The particle streams were viewed and captured separately using appropriate filter cubes.

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Microfluidic Application Notes

Microbiome culture in droplet using dsurf surfactant

The performance of three commonly used surfactants are compared at three different concentrations. The droplet stability over time and the droplet occupation rate is determined by encapsulating a microbial community derived from human skin.

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Chitosan microcapsule
Microfluidic Application Notes

Microfluidic Chitosan Microcapsules Production

In this Application Note, chitosan-shell/oily-core microcapsules are generated using the Raydrop double emulsion chip, and Fluigent pressure-based flow controllers. The influence of the fluidic parameters on the size and the release from the oil across the shell are studied and presented.

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Droplet generation production on water in oil emulsions 2
Microfluidic Application Notes

Production of water-in-oil emulsions using a droplet generator chip

In this application note, we demonstrate droplet generation using the Fluigent microfluidic system including pressure pumps, chemicals, tubing and a Droplet Generator Chip obtained from our partner microfluidic ChipShop.

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alginate bead generation monodispercity
Microfluidic Application Notes

Alginate Microbeads Production

Following is a method for encapsulation of reagent into alginate microbeads with total control of bead formation. A droplet-based microfluidic method is used to precisely control the production of microbeads without the drawbacks of large size distribution that present other methods.

 

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Double emulsion generation
Microfluidic Application Notes

Double Emulsion Generation

In this application, we are going to present a robust capillary-based device that allows single-step production of double emulsion with a single device. Different examples of double emulsion (w/o/w and o/w/o) have been generated to show the versatility and adaptability of the Raydrop to multiple applications such as Polymer and Hydrogel microcapsules for drug delivery or for encapsulation of cells for FACS sorting as well.

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evaluate-cell-growth
Microfluidic Application Notes

How to Evaluate Cell Proliferation Using Pressure as a Tool

This application presents a simple method to monitor cell proliferation in microfluidic chips in real time. This is demonstrated experimentally using a custom microfluidic chip. Cell morphology was studied under flowing and static culture condition

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capillary electrophoresis schematic microfluidic
Microfluidic Application Notes

Capillary electrophoresis using microfluidic, electrophoretic, and optic modules

Here we present here the Lego CE system consisting of available ready-to-use electrophoretic and microfluidic modules, including pressure-based flow controllers. The instrument is coupled with a laser-induced fluorescence detector (LIF) and is demonstrated for separations of labeled oligosaccharides

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characterization of copper
Microfluidic Application Notes

Microfluidics for Transmission Electron Microscopy: Characterization of Copper Electrodeposition

This application presents the Liquid-phase Transmission Electron Microscopy (LPTEM) technique, which integrates integrates liquid flow capabilities within microfabricated liquid cells, providing the means to study different processes in solution with sub-nanometer spatial resolution and sub-microsecond temporal resolution. Using this technique, it is now possible to visually study topics ranging from material science to life science.

 

 

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digital pcr assay
Microfluidic Application Notes

Analysis of a commercial surfactant for digital PCR assay

In this application note we are investigating the usability of the commercially available surfactant dSurf for an exemplary digital PCR-assay.

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Resin-microparticle-SEM2
Microfluidic Application Notes

UV-Crosslinking of Microparticles

In this short note, we present a Fluigent microfluidic solution for polymer microparticle synthesis with high monodispersity (2% CV). The microfluidic system allows for inline particle generation, spacing, and polymerization by UV light.

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Microcapsule-with-shell-thickness-adjustment
Microfluidic Application Notes

UV-Crosslinking of Microcapsules

Using the double emulsion Raydrop, controlled double emulsification is achieved by dripping or jetting the core fluid into an immiscible shell fluid, which is then encapsulated by the third fluid. In this note, capsules are formed by consolidating shell phase of the resulting double emulsions by uv-crosslinking of monomers and photoinitiator used as shell phase.

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Microfluidic Application Notes

Water in Fluorocarbon Oil Emulsions

In this application note, we present droplet generation data obtained using Fluigent’s own fluorocarbon oil formulation – dSURF to demonstrate the ability of Fluigent equipment coupled with Raydrop microfluidic devices to generate high-quality emulsions with controlled droplet sizes and with high throughput.

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Water in oil emulsions
Microfluidic Application Notes

Water in Oil Emulsions

In this application note, we present droplet generation data obtained using one of the most widely used water in oil emulsion systems – water in decane. We demonstrate the ability of Fluigent equipment coupled with RayDrop microfluidic devices to generate high-quality emulsions with controlled droplet sizes and with high throughput.

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Oil in water emulsions
Microfluidic Application Notes

Oil in Water Emulsions

In this application note, we present droplet generation data obtained using decane in Water, a system that demonstrates the expected behavior of most hydrocarbons in Water. We demonstrate the ability of Fluigent equipment coupled with Raydrop microfluidic devices to generate high-quality emulsions with controlled droplet sizes and with high throughput.

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Impedance measurements of microbeads
Microfluidic Application Notes

Impedance Measurement of Microbeads

We present in this application note our Electrical Impedance Spectroscopy Platform (or EISP) consisting of microfluidic flow controllers from Fluigent to maintain precise flow control, a chip from Micronit Microtechnologies B. V to localize impedance measurements, and a lock-in amplifier from Zurich Instruments to perform impedance measurements. We demonstrate the system efficiency by determining the size of micrometer beads and by measuring the generation rate water-in-oil droplets.

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pressure-vs-peristaltic
Microfluidic Application Notes

Peristaltic Pump vs Pressure-Based Microfluidic Flow Control for Organ on Chip applications

The importance of flow stability in microfluidic systems is demonstrated by performing cell culture using peristaltic pumps or pressure-based flow controllers.  

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webinar fluid delivery fluigent
Microfluidic Application Notes

Capture and Labeling of Cancer Cells Using Aria

This application note describes the use of the new Fluigent Aria – a software-assisted instrument capable of delivering up to 10 different solutions – for the automation of a complete protocol of capture and labeling of MDA-MB-231 breast cancer cells including surface treatment, injection of antibodies-coated beads, and cell suspension, and immunostaining steps.

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