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alginate bead generation monodispercity

Application notes

Alginate microbeads production

Following is a method for encapsulation of reagent into alginate microbeads with total control of bead formation. A droplet-based microfluidic method is used to precisely control the production of microbeads without the drawbacks of large size distribution that present other methods.

digital pcr assay

Application notes

Analysis of a commercial surfactant for digital PCR

In this application note we are investigating the usability of the commercially available surfactant dSurf for an exemplary digital PCR-assay.

capillary electrophoresis schematic microfluidic

Application notes

Capillary electrophoresis using microfluidic, electrophoretic, and optic modules

We present here the Lego CE system consisting of available ready-to-use electrophoretic and microfluidic modules, including pressure-based flow controllers. The instrument is coupled with a laser-induced fluorescence detector (LIF) and is demonstrated for separations of labeled oligosaccharides

webinar fluid delivery fluigent

Application notes

Capture and Labeling of cancer cells using Aria

This application note describes the use of the new Fluigent Aria – a software-assisted instrument capable of delivering up to 10 different solutions – for the automation of a complete protocol of capture and labeling of MDA-MB-231 breast cancer cells including surface treatment, injection of antibodies-coated beads, and cell suspension, and immunostaining steps.

Chondrocyte deformation

Application notes

Cartilage on chip using Fluigent MFCS pressure controller

Elucidating how chondrocytes react to external stimuli (mechanical or chemical) is important to understand processes triggering cartilage diseases like osteoarthritis. In this application note, we report on the use of Fluigent products to create complex mechanical stimulation patterns on 3D cell culture in a microfluidic platform, or so-called organ-on-a-chip device, with a specific focus on creating a cartilage-on-a-chip model.

Cell-Perf-System

Application notes

Cell perfusion with pulse-free flow with one manifold

In this application, one Flow EZ™ pressure channel is connected via a manifold to ten separate vials containing different aqueous solutions. Use of the M-Switch™ and OxyGEN allows the selection of a specific solution directed to a microfluidic device.

characterization of copper

Application notes

Characterization of copper electrodeposition in liquid phase electron microscopy

This application presents the Liquid-phase Transmission Electron Microscopy (LPTEM) technique, which integrates integrates liquid flow capabilities within microfabricated liquid cells, providing the means to study different processes in solution with sub-nanometer spatial resolution and sub-microsecond temporal resolution. Using this technique, it is now possible to visually study topics ranging from material science to life science.

Chitosan microcapsule

Application notes

Chitosan microcapsules production

In this Application Note, chitosan-shell/oily-core microcapsules are generated using the Raydrop double emulsion chip, and Fluigent pressure-based flow controllers. The influence of the fluidic parameters on the size and the release from the oil across the shell are studied and presented.

gut-on-chip caco 2 monolayer

Application notes

Development of a human gut-on-chip to assess the effect of shear stress on intestinal functions

Fluid flow and shear stress have previously been reported to promote proper intestinal cell differentiation, formation of villus-like 3D structures and enhanced intestinal barrier function.

Double emulsion generation

Application notes

Double Emulsion Production

In this application, we are going to present a robust capillary-based device that allows single-step production of double emulsion with a single device. Different examples of double emulsion (w/o/w and o/w/o) have been generated to show the versatility and adaptability of the Raydrop to multiple applications such as Polymer and Hydrogel microcapsules for drug delivery or for encapsulation of cells for FACS sorting as well.

dropseq method

Application notes

Drop-Seq method

The Drop-Seq protocol, is a high throughput method that enables the sequencing of the mRNA from a large number of cells. With this method it is possible to create a gene expression map of the cell, or even distinguish cell populations within a tissue!

WaterInOil-schema

Application notes

Droplet and particle manipulation using electrophoretic flow control

Many microfluidic applications are developed based on concepts relying on electrodes, exploiting the electrical properties of samples to sort or separate them, generate electro-osmotic flows, manipulate particles, perform electrochemical detection etc. One example of this is electronic paper, where the electrophoretic displacement of titanium dioxide particles in a dark hydrocarbon oil solution allows the formation of a black and white pattern with a high resolution.

dSurf-Droplet2

Application notes

E. Coli culture in droplets using dSURF fluorosurfactant

In this application note, discover how using microfluidics allows one to encapsulate single or multiple cells into tiny droplets of pL volume which are generated at a rate of approximately one thousand per second.

recirculation microfluidic

Application notes

Fluid recirculation for cell perfusion with reduced shear stress

Many microfluidic applications require expensive solutions to be injected at a controlled flow-rate into a microfluidic system, such as cell cultures, PCR processes, cell injections or simulation of blood capillaries with a controlled minimal mechanical stress.

nozzle for bubble generation

Application notes

Generation of bubbles using the RayDrop

Microfluidic devices are used for Bubble generation at micron-scale, yielding high monodispersity and well-controlled size. We investigated how parameters such as the geometry of the nozzle and the continuous-phase flow rate affects the microbubble formation process.

Microcapsule-with-shell-thickness-adjustment

Application notes

Generation of microcapsules with a UV-crosslinked polymer

Using the double emulsion Raydrop, controlled double emulsification is achieved by dripping or jetting the core fluid into an immiscible shell fluid, which is then encapsulated by the third fluid. In this note, capsules are formed by consolidating shell phase of the resulting double emulsions by uv-crosslinking of monomers and photoinitiator used as shell phase.

Resin-microparticle-SEM2

Application notes

Generation of microparticle with a UV-crosslinked polymer

In this short note, we present a Fluigent microfluidic solution for polymer microparticle synthesis with high monodispersity (2% CV). The microfluidic system allows for inline particle generation, spacing, and polymerization by UV light.

High-throughput-cell-DNA-encapsulation-graph

Application notes

High-throughput cell DNA screening using digital PCR

The use of water-in-oil droplets in microfluidics in high-throughput screening is rapidly gaining acceptance. The main application areas currently involve screening cells as well as genetic material for various mutations or activity. Thanks to droplet microfluidic, Michael Ryckelynck and his team are able to isolate single DNA molecules and analyze the enzymes and proteins resulting from their expression.

Impedance measurements of microbeads

Application notes

Impedance spectroscopy for characterization and counting

We present in this application note our Electrical Impedance Spectroscopy Platform (or EISP) consisting of microfluidic flow controllers from Fluigent to maintain precise flow control, a chip from Micronit Microtechnologies B. V to localize impedance measurements, and a lock-in amplifier from Zurich Instruments to perform impedance measurements. We demonstrate the system efficiency by determining the size of micrometer beads and by measuring the generation rate water-in-oil droplets.

Application notes

Liposome nanoparticles synthesis

Liposomes were discovered in the 1960s. These hollow nanoparticles are phospholipid vesicles consisting of at least one lipid bilayer. This bilayer is usually composed of amphiphilicphospholipids that have a hydrophilic phosphate head and a hydrophobic tail consisting of two fatty acid chains. This structural feature has facilitated liposomes’ applications, including their use as artificial cell membranes, carriers for drug delivery systems, encapsulating agents forfood ingredients, and analytical tools.

Application notes

Microbiome culture in droplet using dsurf surfactant

The performance of three commonly used surfactants are compared at three different concentrations. The droplet stability over time and the droplet occupation rate is determined by encapsulating a microbial community derived from human skin.

microfluidic cell sorting

Application notes

Microfluidic platform for cell and particle sorting applications

Passive cell or particle sorting using a dedicated package. To demonstrate the separation of particle mixtures, a solution containing 7.5 µm and 15 µm diameter polystyrene particles labeled with FITC, and TRITC fluorophores respectively was used. The particle streams were viewed and captured separately using appropriate filter cubes.

Application notes

Multiple emulsion droplet generation

How to encapsulate multiple aqueous droplets (called “core”) into a single oil shell.

Oil in water emulsions

Application notes

Oil in Water Emulsions

In this application note, we present droplet generation data obtained using decane in Water, a system that demonstrates the expected behavior of most hydrocarbons in Water. We demonstrate the ability of Fluigent equipment coupled with Raydrop microfluidic devices to generate high-quality emulsions with controlled droplet sizes and with high throughput.

pressure-vs-peristaltic

Application notes

Peristaltic pump vs pressure-based microfluidic flow control systems for Organ on-chip applications

Microfluidic cell culture has significant advantages over macroscopic culture in flasks, Petri dishes, and well-plates. To demonstrate the importance of flow stability in vascular models, endothelial cells seeded in microfluidic chips were perfused either using a peristaltic pump or pressure-based flow controllers.

monodisperse plga double emulsion

Application notes

PLGA microcapsule synthesis

In this Application Note, PLGA shell/aqueous core microcapsules are obtained using the Secoya Raydrop Double emulsion. The influence of the fluidic parameters on the microcapsule size and release from the oil across the shell are explored in this application note.

Application notes

PLGA microparticle synthesis

When PLGA is used as an active pharmaceutical ingredient carrier it is important to produce highly monodispersed particles for drug release reproducibility. The most common production process of PLGA particles is solvent based and can involve hazardous solutions. Ethyl acetate is preferred as it shows better biocompatibility than other conventional solvent such as dichloromethane.

plga nanoparticle synthesis acetone and water pva

Application notes

PLGA nanoparticle synthesis using 3D microfluidic hydrodynamic focusing

In this Application Note, PLGA nanoparticles with high monodispersity are generated using the Raydrop single emulsion developed and manufactured by Secoya, and Fluigent pressure-based flow controllers. The ability to synthesise PLGA nanoparticles in a more controllable and reproducible way creates possibilities to tailor surface properties and increase fields of application.

evaluate-cell-growth

Application notes

Pressure as a tool to evaluate cell growth

This application presents a simple method to monitor cell proliferation in microfluidic chips in real time. This is demonstrated experimentally using a custom microfluidic chip. Cell morphology was studied under flowing and static culture condition

Application notes

Pressure predictions for lab-on-a-chip operations using a microfluidic network solver and Fluigent PX

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Droplet generation production on water in oil emulsions 2

Application notes

Production of water-in-oil emulsions using a droplet generator chip

In this application note, we demonstrate droplet generation using the Fluigent microfluidic system including pressure pumps, chemicals, tubing and a Droplet Generator Chip obtained from our partner microfluidic ChipShop.

Application notes

Water in Fluorocarbon Oil Emulsions

In this application note, we present droplet generation data obtained using Fluigent’s own fluorocarbon oil formulation – dSURF to demonstrate the ability of Fluigent equipment coupled with Raydrop microfluidic devices to generate high-quality emulsions with controlled droplet sizes and with high throughput.

Water in oil emulsions

Application notes

Water in Oil Emulsions

In this application note, we present droplet generation data obtained using one of the most widely used water in oil emulsion systems – water in decane. We demonstrate the ability of Fluigent equipment coupled with RayDrop microfluidic devices to generate high-quality emulsions with controlled droplet sizes and with high throughput.