Oil in Water Emulsions

Oil in Water emulsions (O/W) are widely used in industrial and R&D environments to manufacture droplets (e.g., for compartmentalization applications), wax beads (e.g., carnauba or cosmetic wax), and polymer beads (e.g., PLGA, Styrene, methacrylates, etc.). Of particular importance is the ability to produce high-quality, monodisperse droplets and the ability to do so reproducibly and at a viable production rate.

The combination of Pressure based flow controller units and RayDrop microfluidic devices developed and manufactured by Secoya enable smooth fluid delivery, precision flowrate control, automation, and reproducibility necessary to generate high-quality oil in water emulsions.


We kindly thank of SMALL Biotechnologies for this collaboration


Developed and manufactured by Secoya

Materials and methods


Droplet Phase: Decane

Continuous phase: Water + 2% (wt) Sodium dodecyl sulfate (SDS)

ReagentSupplierCatalogue numberCAS Number
WaterUltrapure 18.2 MΩ – cm7732-18-5
DecaneSigma AldrichD901124-18-5
Sodium dodecyl sulfateSigma Aldrich436143151-21-3

Microfluidic Setup

The microfluidic setup was composed of:

Protocols steps

Scheme of the fluidic setup
Figure 1: Scheme of the fluidic setup
Pictures of the Fluigent equipment
Figure 2: Pictures of the Fluigent equipment


Continuous phase flowrate (μl/min)Droplet phase flowrate
Droplet diameter
Production rate
Results-OIL-IN-WATER-droplet phase diagram
Figure 3: Droplet phase diagram
Results-OIL-IN-WATER-decane droplet in water
Figure 4: Images of decane droplets in water generated using Fluigent equipment and Raydrop microfluidic device


Fluigent pressure-based flow controller units and Raydrop microfluidic device were successfully used to generate high-quality, monodisperse droplets of decane in water. The droplet size was controlled in the range of 63 – 83 μm by adjusting the continuous and dispersed phase flowrates. Peak stable droplet production rate was recorded for 73 μm droplets at 1227 Hz. The production techniques developed here can be extended to the generation of wax, p, or polymer beads by adding suitable post-processing steps.

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