Expert Reviews: Basics of Microfluidics
- Microfluidics tips
- Industrial / OEM Microfluidic Expertise
- A general overview of microfluidics
- Advantages of pressure-based microfluidics
- Elements of a microfluidic system
- Concepts and physics of microfluidics
- Droplet and particle generation in microfluidics
- Microfluidic cell biology
- Funded research program participation
Addressing Air Bubble Issues in Microfluidic Systems
In this paper, we explore the causes of bubble formation, their effects, and various strategies to deal with this issue. It includes an in-depth discussion on preventive measures like optimizing device design and configuration, as well as the use of Degassers and Bubble traps.
Choosing the Right Microfluidic Pressure Range
Fluigent offers 10 pressure ranges for its different product lines. Which is the right one for you?
Extended capabilities of pressure driven flow for microfluidics applications
Microfluidic experiment often require a high level of control of fluids to permits reproducible results and the success of different application. To this purpose Pressure based flow controller are well suited for a various number of microlfuidic application when high stability is required such as cell perfusion and shear stress control or in droplet production when monodispersity is of high importance.
It also allows to generate complex flow profil such as sequential injection with aortic profil or when real time flow rate measurement is needed, a parameter that other fluid handling system can't offer.
Flow control for droplet generation using syringe pumps and pressure-based flow controllers
Droplet production using microfluidic systems was implemented for applications where monodispersity is of high importance. Syringe pumps are commonly used for generating droplets in microfluidic experiments, but can show limited flow control.. An alternative to syringe pumps are pressure-based flow controllers.
Funded research program participation
Many national and internationally funded projects bring together science and innovation. These projects cover a large number of subjects. Fluigent takes part in several European and French funded programs to provide expertise and resources in microfluidics and related applications.
Giant Unilamellar Vesicles (GUVs) Production using Microfluidics
In this review, we propose an overview of the different techniques used to produce microsized aqueous compartments confined by a single lipid bilayer that separates them from the surrounding aqueous environment.
High Throughput Single Cell Analysis
Microfluidics and lab-on-a-chip technology have emerged as the most promising approaches to address these challenges. These innovative technologies hold the potential to unlock new insights into single cell properties and their roles within populations.
How to choose a microfluidic chip
With this review, we give you some advices on how to select the right microfluidic chip for your application.
How to reproduce active biomimetic stimulation in vitro?
Organ-on-a-chip (OOC) technology has paved the way for investigating the impact of mechanical strain in cell biology research by reproducing key aspects of an in vivo cellular microenvironment. Combining microfluidics and microfabrication enables one to reproduce mechanical forces experienced by living tissues at the cell scale.
INDEX | H2020 European project
INDEX: Integrated nanoparticle isolation and detection system for complete on-chip analysis of exosomes The aim of the European Union’s Horizon H2020 project, INDEX is to isolate and characterize exosomes available in body fluids through development and integration of novel technological breakthroughs.
Instrument Stability in Microfluidics
In microfluidic instrumentation, stability refers to the ability of an instrument to maintain a certain physical property at a constant value, while rejecting any perturbations in the environment. It is a very important parameter, as even small variations in physical quantities during microfluidic experiments can dramatically change the results. A stable instrument ensures that results are repeatable and reproducible.
Key considerations for fluidic system integration
The choice of the right method of fluid management can often determinate the success of a project, as well as the overall time and cost of manufacturing. Here is what you need to consider and be aware of when integrating fluidic control into your system.
Key reliability indicators for OEM components to ensure long-term performance of your flow control system
In this short blog, we review the most important aspects to consider when selecting fluid handling OEM components.
Mastering Microfluidic Chips: An In-Depth Definition
Microfluidic chips, often referred to as lab-on-a-chip devices, are miniature platforms that manipulate and analyze small volumes of fluids. We here define microfluidic chips and summarize microfluidic history.
Microfabrication of Microfluidic Chips: Materials and Methods
We discuss the main materials used for microfluidic chips, and their related production methods. In addition, we give you advice on which material one should use depending on the application.
Microfluidic chips: key applications
We discuss some applications where microfluidics achieves results that would be very challenging to obtain when using conventional methods.
Microfluidic Droplet Production Method
Droplet microfluidics is a powerful tool which consists of generating and manipulating micron-sized monodispersed droplets.
Microfluidic Flow Control Technologies: Strengths and Weaknesses
To perform effective experiments in microfluidics, one needs to master the different flow control technologies available to use the most suitable way to control microfluidic flows. This article aims at presenting a short review of the existing techniques and their strengths and weaknesses.
Microfluidic Flow Control: Comparison between peristaltic, syringe and pressure pumps for microfluidic applications
To perform effective experiments in microfluidics, one needs to master the different flow control technologies available to use the most suitable way to control microfluidic flows. This article aims at presenting a short review of the existing techniques.
Microfluidic Flow Sensing Technologies, A Review
Flow rate measurement is needed for many microfluidic applications, including microfluidic droplet generation, cell culture under flow, and organs on chip. Several technologies aim to provide accurate real time flow measurement to meet this need. In this review, the different existing microfluidic flow sensing solutions for low-flow liquids are described, with their advantages and current limits, as progress is still ongoing in this field.
Microfluidic instrument responsiveness
In microfluidics, response time is the duration between a command and the the start of flow (the first reaction of the microfluidic pump). When using a pressure controller, flow will start once a sufficient pressure rise occurs in the fluidic reservoir. The electrical and mechanical response times of the hardware components such as valves need to be as low as possible in order to decrease the response time.
Microfluidic pressure control for organ-on-a-chip applications: A comprehensive guide
This comprehensive guide will cover several essential definitions, advantages, and disadvantages of different technologies, considerations for choosing the right technology, and examples of OOC using Fluigent devices.
Microfluidic flows are characterized by the prevalence of the viscous effects compared to inertia. From a physics point of view, this behavior is pointed out by a low Reynolds number indicating laminar flow. It leads to a drastic simplification of the complex Navier-Stokes equations describing fluid displacement.
Several parameters must be taken into consideration in order to choose the tubing:
- Tubing dimensions such as the outer diameter“OD”, the inner diameter “ID” and the length “L”. For example the inner diameter plays a significant role in the resistivity brought by the tubing: the smaller it is, the more resistant the tubing will be...
Microfluidic volume definitions
Most of the time, it is useful to know the total volume of one’s fluidic circuit. Volumes in microfluidics can be different from other areas. The chip has its own volume, tubing has an internal volume. Fittings like unions, adapters or tees can have an enclosed volume that will contribute to the total volume of a system. This is referred in specification sheets as “internal volume”.
Microfluidics definitions and advantages
Microfluidics is the science of manipulating and controlling fluids, usually in the range of microliters (10-6) to picoliters (10-12), in networks of channels with dimensions from tens to hundreds of micrometers.
Microfluidics for vaccine development
Microfluidic methods can be used to improve vaccine research and development. Microfluidic techniques are already used to develop adjuvants, perform virus identification/diagnostics, or drug micro and nanoencapsulation.
Micropipette aspiration of cells and tissues
Micropipette aspiration is a powerful non-invasive technique to evaluate how biomechanical properties of single cells or tissue govern cell shape, cell response to mechanic stimuli, transition from nontumorigenic to tumorigenic state or morphogenesis
Mimicking in-vivo environments: biochemical and biomechanical stimulation
Cells are constantly exposed to biochemical stimulation from the early embryonic stage to adult life. The spatiotemporal regulation of these signals is essential as it determines cell fate, phenotype, metabolic activity as well as pathological behaviors. The fast response and high stability of Fluigent instruments make them the best solution available on the market to reproduce these complex variations in vitro.
MYOCHIP | H2020 European project
Myochip: Building a 3D innervated and irrigated muscle on a chip
The aim of the European Union’s Horizon H2020 project, Myochip is to build a muscle on chip. Myochip gathers 4 partners from all around the world: The institute of molecular medicine (Portugal), The University of Edinburg (Scotland), and Institut Curie (France).
Passive and active mechanical stimulation in microfluidic systems
Indirect mechanical forces and shear stress are integral parts of the cellular microenvironment. Reproducing these mechanical forces and shear stress is critical to capture the physiology of living tissues. Three types of flows are typically generated for producing shear stress : laminar, pulsatile, or interstitial flow.
Prostate Organoid Culture in Microbeads
Microbead-based microfluidics is a powerful technique that generates highly monodispersed picoliter-sized beads into a continuous phase. This method has been successfully adapted to cell culture to encapsulate cells in micron size hydrogel beads. The main advantages are reduced costs related to miniaturization, high reproducibility, and high throughput screening capacities.
Software in Microfluidics
Software is widely defined as a set of computer programs, libraries and data that tell the computer how to work. A user can usually interact with a software through a Graphical User Interface or GUI, whose content is updated by the software’s engine. Our software tools are mostly dedicated to controlling our microfluidics instruments, allowing for remote instruments’ control and sensor’ data logging.
The Raydrop | A new droplet generation device based on non-embedded co-flow-focusing
has developed a new system to overcome the limitations of common designs used in droplet generation chips.
What is the history of microfluidics?
Microfluidics is both the science which studies the behaviour of fluids through micro-channels, and the technology of systems that process or manipulate small (10-6 to 10-12 litres) amounts of fluids using microminiaturized devices
Why is a controlled shear-stress a key parameter of your microfluidic experiments?
Mechanical forces are potent regulators of cellular structures and functions in both health and disease. Of these forces, shear stress is particularly important as it stimulates the release of vasoactive substances and changes gene expression, cell metabolism, and cell morphology.