Cells are constantly exposed to biochemical stimulation from the early embryonic stage to adult life. The spatiotemporal regulation of these signals is essential as it determines cell fate, phenotype, metabolic activity as well as pathological behaviors. The fast response and high stability of Fluigent instruments make them the best solution available on the market to reproduce these complex variations in vitro.
In vivo, most cells are constantly exposed, actively or passively, to mechanical forces. Reproducing these physiological constraints in vitro is essential to induce the right phenotype to cells, finalize their maturation and maintain homeostasis. The wide range of pressure covered by Fluigent products permits one to accurately study biomechanics from molecular level to organ scale.
Choosing the right pressure range
Fluigent offers 10 pressure ranges for its different product lines. Which is the right one for you?
Comparison between peristaltic, syringe and pressure pumps for microfluidic applications
This pump selection guide shows the advantages as well as the disadvantages of each method of fluid delivery in Microfluidics so it will help you to choose the proper one for your microflui
Droplet generation using syringe pumps and pressure-based flow controllers
Droplet production using microfluidic systems was implemented for applications where monodispersity is of high importance. Syringe pumps are commonly used for generating droplets in microfluidic experiments, but can show limited flow control.. An alternative to syringe pumps are pressure-based flow controllers.
Extended capabilities of pressure driven flow for microfluidics applications
Microfluidic experiment often require a high level of control of fluids to permits reproducible results and the success of different application. To this purpose Pressure based flow controller are well suited for a various number of microlfuidic application when high stability is required such as cell perfusion and shear stress control or in droplet production when monodispersity is of high importance.
It also allows to generate complex flow profil such as sequential injection with aortic profil or when real time flow rate measurement is needed, a parameter that other fluid handling system can't offer.
Flow control and measurement
The main flow control solutions can be divided in three sections: pressure based solutions (such as the Flow EZ™), volume displacement (such as syringe or peristaltic pumps) and passive techniques. All of these control techniques have different advantages. In order to make the best choice, it is important to consider the following elements.
- The flow rate or the pressure range you need.
- How quickly you need to set or change the flow rate.
- How stable you need the flow rate to be.
Flow Control Technologies
To perform effective experiments in microfluidics, one needs to master the different flow control technologies available to use the most suitable way to control microfluidic flows. This article aims at presenting a short review of the existing techniques.
Funded research program participation
Many national and internationally funded projects bring together science and innovation. These projects cover a large number of subjects. Fluigent takes part in several European and French funded programs to provide expertise and resources in microfluidics and related applications.
High throughput single cell analysis
Individual cell heterogeneity within a population has invalidated historic classification methods based on macroscopic considerations and given rise to new evaluation techniques based on single cell transcriptional signature. In this context, thanks to high throughput screening capacities, easy fluid handling and reduced costs related to device miniaturization, microfluidics has emerged as a powerful tool for single cell manipulation and analysis.
How to ask cells what proteins they produce?
The conventional way to isolate cells is by using Fluorescence Activation (FACS) to sort them into barcoded well plates (96 wells, 384 or 1536). This step is quite fast with the FACS currently available, but segregating thousands of cells into plates will result in a long process of pipetting. Droplet microfluidics allows for fast and low volume compartmentalization up to a thousand drops produced per second
How to choose a microfluidic chip
With this review, we give you some advices on how to select the right microfluidic chip for your application.
How to reproduce active biomimetic stimulation in vitro?
Organ-on-a-chip (OOC) technology has paved the way for investigating the impact of mechanical strain in cell biology research by reproducing key aspects of an in vivo cellular microenvironment. Combining microfluidics and microfabrication enables one to reproduce mechanical forces experienced by living tissues at the cell scale.
INDEX | H2020 European project
INDEX: Integrated nanoparticle isolation and detection system for complete on-chip analysis of exosomes The aim of the European Union’s Horizon H2020 project, INDEX is to isolate and characterize exosomes available in body fluids through development and integration of novel technological breakthroughs.
Key indicators to ensure long-term performance of your OEM flow control components
In this short blog, we review the most important aspects to consider when selecting fluid handling OEM components.
Microfluidic chips: key applications
We discuss some applications where microfluidics achieves results that would be very challenging to obtain when using conventional methods.
Microfluidic chips: materials and production methods
We discuss the main materials used for microfluidic chips, and their related production methods. In addition, we give you advice on which material one should use depending on the application.
Microfluidic Droplet Production Method
Droplet microfluidics is a powerful tool which consists of generating and manipulating micron sized monodispersed droplets. A Microfluidic based droplet has many diverse and varied applications such as particle synthesis and physicochemical analysis .A good control of droplet production can also make single-cell analysis, or drug testing possible. In this review we are presenting all microfluidic methods that can be used to produce droplets.
Microfluidic Flow Control Technologies: Strengths and Weaknesses
To perform effective experiments in microfluidics, one needs to master the different flow control technologies available to use the most suitable way to control microfluidic flows. This article aims at presenting a short review of the existing techniques and their strengths and weaknesses.
Microfluidic instrument responsiveness
In microfluidics, response time is the duration between a command and the the start of flow (the first reaction of the microfluidic pump). When using a pressure controller, flow will start once a sufficient pressure rise occurs in the fluidic reservoir. The electrical and mechanical response times of the hardware components such as valves need to be as low as possible in order to decrease the response time.
Microfluidic Instrument Stability
In microfluidic instrumentation, stability refers to the ability of an instrument to maintain a certain physical property at a constant value, while rejecting any perturbations in the environment. It is a very important parameter, as even small variations in physical quantities during microfluidic experiments can dramatically change the results. A stable instrument ensures that results are repeatable and reproducible.
Microfluidic flows are characterized by the prevalence of the viscous effects compared to inertia. From a physics point of view, this behavior is pointed out by a low Reynolds number indicating laminar flow. It leads to a drastic simplification of the complex Navier-Stokes equations describing fluid displacement.
Several parameters must be taken into consideration in order to choose the tubing:
- Tubing dimensions such as the outer diameter“OD”, the inner diameter “ID” and the length “L”. For example the inner diameter plays a significant role in the resistivity brought by the tubing: the smaller it is, the more resistant the tubing will be...
Microfluidic volume definitions
Most of the time, it is useful to know the total volume of one’s fluidic circuit. Volumes in microfluidics can be different from other areas. The chip has its own volume, tubing has an internal volume. Fittings like unions, adapters or tees can have an enclosed volume that will contribute to the total volume of a system. This is referred in specification sheets as “internal volume”.
Microfluidics definitions and advantages
Microfluidics is the science of manipulating and controlling fluids, usually in the range of microliters (10-6) to picoliters (10-12), in networks of channels with dimensions from tens to hundreds of micrometers.
Microfluidics for vaccines research and development
Microfluidic methods can be used to improve vaccine research and development. Microfluidic techniques are already used to develop adjuvants, perform virus identification/diagnostics, or drug micro and nanoencapsulation.
Micropipette cell and tissue aspiration
Micropipette aspiration is a powerful non-invasive technique to evaluate how biomechanical properties of single cells or tissue govern cell shape, cell response to mechanic stimuli, transition from nontumorigenic to tumorigenic state or morphogenesis
MYOCHIP | H2020 European project
Myochip: Building a 3D innervated and irrigated muscle on a chip
The aim of the European Union’s Horizon H2020 project, Myochip is to build a muscle on chip. Myochip gathers 4 partners from all around the world: The institute of molecular medicine (Portugal), The University of Edinburg (Scotland), and Institut Curie (France).
Organoid Culture in micro-beads
Microbead-based microfluidics is a powerful technique that generates highly monodispersed picoliter-sized beads into a continuous phase. This method has been successfully adapted to cell culture to encapsulate cells in micron size biocompatible hydrogel beads.
Passive mechanical stimulation induced by laminar and pulsatile shear stress
Indirect mechanical forces and shear stress are integral parts of the cellular microenvironment. Reproducing these mechanical forces and shear stress is critical to capture the physiology of living tissues. Three types of flows are typically generated for producing shear stress : laminar, pulsatile, or interstitial flow.
Software in Microfluidics
Software is widely defined as a set of computer programs, libraries and data that tell the computer how to work. A user can usually interact with a software through a Graphical User Interface or GUI, whose content is updated by the software’s engine. Our software tools are mostly dedicated to controlling our microfluidics instruments, allowing for remote instruments’ control and sensor’ data logging.
The Raydrop: A new droplet generation device based on non‑embedded co‑flow‑focusing
After introducing the Raydrop, Adrien Dewandre and his team from ULB demonstrate that this new configuration offers the ability to emulsify any liquid with a wide range of droplet sizes. This universality is demonstrated with experimental results. Modeling is also performed, supporting experimental results, and allowing for the prediction of droplet size and production regime (dripping, jetting regimes).
Things you should know when integrating fluidics into your system
The choice of the right method of fluid management can often determinate the success of a project, as well as the overall time and cost of manufacturing. Here is what you need to consider and be aware of when integrating fluidic control into your system.
Volumetric Control Technologies
To perform effective experiments in microfluidics, one needs to be aware of the different volumetric control technologies available to use the most suitable way to control microfluidic flows. The present article presents review of the existing techniques.
Here is a list of the main microfluidic flow control technologies.
What is a microfluidic chip?
We here define microfluidic chips and summarize microfluidic history.
What is the history of microfluidics?
Microfluidics is both the science which studies the behaviour of fluids through micro-channels, and the technology of systems that process or manipulate small (10-6 to 10-12 litres) amounts of fluids using microminiaturized devices
Why is it important to control shear stress in your microfluidic experiments?
Mechanical forces are potent regulators of cellular structures and functions in both health and disease. Of these forces, shear stress is particularly important as it stimulates the release of vasoactive substances and changes gene expression, cell metabolism, and cell morphology.