专业知识回顾

Search by name
Products
Industrial apps
Categories

微流控细胞生物学

Biochemical Environment

Cells are constantly exposed to biochemical stimulation from the early embryonic stage to adult life. The spatiotemporal regulation of these signals is essential as it determines cell fate, phenotype, metabolic activity as well as pathological behaviors. The fast response and high stability of Fluigent instruments make them the best solution available on the market to reproduce these complex variations in vitro.

微流控细胞生物学

Biomechanics

In vivo, most cells are constantly exposed, actively or passively, to mechanical forces. Reproducing these physiological constraints in vitro is essential to induce the right phenotype to cells, finalize their maturation and maintain homeostasis. The wide range of pressure covered by Fluigent products permits one to accurately study biomechanics from molecular level to organ scale.

微流控小知识

Choosing the right pressure range

Fluigent offers 10 pressure ranges for its different product lines. Which is the right one for you?

什么是微流控技术？

Comparison between peristaltic, syringe and pressure pumps for microfluidic applications

This pump selection guide shows the advantages as well as the disadvantages of each method of fluid delivery in Microfluidics so it will help you to choose the proper one for your microflui

基于压强的微流控技术的优势

Droplet generation using syringe pumps and pressure-based flow controllers

Droplet production using microfluidic systems was implemented for applications where monodispersity is of high importance. Syringe pumps are commonly used for generating droplets in microfluidic experiments, but can show limited flow control.. An alternative to syringe pumps are pressure-based flow controllers.

基于压强的微流控技术的优势

Extended capabilities of pressure driven flow for microfluidics applications

Microfluidic experiment often require a high level of control of fluids to permits reproducible results and the success of different application. To this purpose Pressure based flow controller are well suited for a various number of microlfuidic application when high stability is required such as cell perfusion and shear stress control or in droplet production when monodispersity is of high importance.

It also allows to generate complex flow profil such as sequential injection with aortic profil or when real time flow rate measurement is needed, a parameter that other fluid handling system can't offer.

微流控系统的要素

Flow control and measurement

The main flow control solutions can be divided in three sections: pressure based solutions (such as the Flow EZ™), volume displacement (such as syringe or peristaltic pumps) and passive techniques. All of these control techniques have different advantages. In order to make the best choice, it is important to consider the following elements.

The flow rate or the pressure range you need.
How quickly you need to set or change the flow rate.
How stable you need the flow rate to be.

微流控技术的定义和物理性质

Flow Control Technologies

To perform effective experiments in microfluidics, one needs to master the different flow control technologies available to use the most suitable way to control microfluidic flows. This article aims at presenting a short review of the existing techniques.

专业知识回顾

Funded research program participation

Many national and internationally funded projects bring together science and innovation. These projects cover a large number of subjects. Fluigent takes part in several European and French funded programs to provide expertise and resources in microfluidics and related applications.

微流控技术中的液滴和微粒制备

High Throughput Single Cell Analysis

Individual cell heterogeneity within a population has invalidated historic classification methods based on macroscopic considerations and given rise to new evaluation techniques based on single cell transcriptional signature. In this context, thanks to high throughput screening capacities, easy fluid handling and reduced costs related to device miniaturization, microfluidics has emerged as a powerful tool for single cell analysis.

微流控技术中的液滴和微粒制备

How to ask cells what proteins they produce?

The conventional way to isolate cells is by using Fluorescence Activation (FACS) to sort them into barcoded well plates (96 wells, 384 or 1536). This step is quite fast with the FACS currently available, but segregating thousands of cells into plates will result in a long process of pipetting. Droplet microfluidics allows for fast and low volume compartmentalization up to a thousand drops produced per second

Microfluidic chip

微流控芯片

How to choose a microfluidic chip

With this review, we give you some advices on how to select the right microfluidic chip for your application.

微流控细胞生物学

How to reproduce active biomimetic stimulation in vitro?

Organ-on-a-chip (OOC) technology has paved the way for investigating the impact of mechanical strain in cell biology research by reproducing key aspects of an in vivo cellular microenvironment. Combining microfluidics and microfabrication enables one to reproduce mechanical forces experienced by living tissues at the cell scale.

资助类研究项目的参与

INDEX | H2020 European project

INDEX: Integrated nanoparticle isolation and detection system for complete on-chip analysis of exosomes The aim of the European Union’s Horizon H2020 project, INDEX is to isolate and characterize exosomes available in body fluids through development and integration of novel technological breakthroughs.

oem flow controller component performance

工业/OEM专业知识

Key reliability indicators for OEM components to ensure long-term performance of your flow control system

In this short blog, we review the most important aspects to consider when selecting fluid handling OEM components.

微流控芯片

Microfabrication of Microfluidic Chips: Materials and Methods

We discuss the main materials used for microfluidic chips, and their related production methods. In addition, we give you advice on which material one should use depending on the application.

微流控芯片

Microfluidic chips: key applications

We discuss some applications where microfluidics achieves results that would be very challenging to obtain when using conventional methods.

微流控技术中的液滴和微粒制备

Microfluidic Droplet Production Method

Droplet microfluidics is a powerful tool which consists of generating and manipulating micron sized monodispersed droplets. A Microfluidic based droplet has many diverse and varied applications such as particle synthesis and physicochemical analysis .A good control of droplet production can also make single-cell analysis, or drug testing possible. In this review we are presenting all microfluidic methods that can be used to produce droplets.

微流控技术的定义和物理性质

Microfluidic Flow Control Technologies: Strengths and Weaknesses

To perform effective experiments in microfluidics, one needs to master the different flow control technologies available to use the most suitable way to control microfluidic flows. This article aims at presenting a short review of the existing techniques and their strengths and weaknesses.

microfluidic flow sensing products

微流控系统的要素

Microfluidic Flow Sensing Technologies, A Review

Flow rate measurement is needed for many microfluidic applications, including microfluidic droplet generation, cell culture under flow, and organs on chip. Several technologies aim to provide accurate real time flow measurement to meet this need. In this review, the different existing microfluidic flow sensing solutions for low-flow liquids are described, with their advantages and current limits, as progress is still ongoing in this field.

基于压强的微流控技术的优势

Microfluidic instrument responsiveness

In microfluidics, response time is the duration between a command and the the start of flow (the first reaction of the microfluidic pump). When using a pressure controller, flow will start once a sufficient pressure rise occurs in the fluidic reservoir. The electrical and mechanical response times of the hardware components such as valves need to be as low as possible in order to decrease the response time.

基于压强的微流控技术的优势

Microfluidic Instrument Stability

In microfluidic instrumentation, stability refers to the ability of an instrument to maintain a certain physical property at a constant value, while rejecting any perturbations in the environment. It is a very important parameter, as even small variations in physical quantities during microfluidic experiments can dramatically change the results. A stable instrument ensures that results are repeatable and reproducible.

微流控技术的定义和物理性质

Microfluidic Resistance

Microfluidic flows are characterized by the prevalence of the viscous effects compared to inertia. From a physics point of view, this behavior is pointed out by a low Reynolds number indicating laminar flow. It leads to a drastic simplification of the complex Navier-Stokes equations describing fluid displacement.

微流控系统的要素

Microfluidic Tubing

Several parameters must be taken into consideration in order to choose the tubing:

Materials
Tubing dimensions such as the outer diameter“OD”, the inner diameter “ID” and the length “L”. For example the inner diameter plays a significant role in the resistivity brought by the tubing: the smaller it is, the more resistant the tubing will be...

微流控技术的定义和物理性质

Microfluidic volume definitions

Most of the time, it is useful to know the total volume of one’s fluidic circuit. Volumes in microfluidics can be different from other areas. The chip has its own volume, tubing has an internal volume. Fittings like unions, adapters or tees can have an enclosed volume that will contribute to the total volume of a system. This is referred in specification sheets as “internal volume”.

什么是微流控技术？

Microfluidics definitions and advantages

Microfluidics is the science of manipulating and controlling fluids, usually in the range of microliters (10-6) to picoliters (10-12), in networks of channels with dimensions from tens to hundreds of micrometers.

专业知识回顾

Microfluidics for vaccines research and development

Microfluidic methods can be used to improve vaccine research and development. Microfluidic techniques are already used to develop adjuvants, perform virus identification/diagnostics, or drug micro and nanoencapsulation.

微流控细胞生物学

Micropipette aspiration of cells and tissues

Micropipette aspiration is a powerful non-invasive technique to evaluate how biomechanical properties of single cells or tissue govern cell shape, cell response to mechanic stimuli, transition from nontumorigenic to tumorigenic state or morphogenesis

资助类研究项目的参与

MYOCHIP | H2020 European project

Myochip: Building a 3D innervated and irrigated muscle on a chip

The aim of the European Union’s Horizon H2020 project, Myochip is to build a muscle on chip. Myochip gathers 4 partners from all around the world: The institute of molecular medicine (Portugal), The University of Edinburg (Scotland), and Institut Curie (France).

微流控细胞生物学

Passive mechanical stimulation induced by laminar and pulsatile shear stress

Indirect mechanical forces and shear stress are integral parts of the cellular microenvironment. Reproducing these mechanical forces and shear stress is critical to capture the physiology of living tissues. Three types of flows are typically generated for producing shear stress : laminar, pulsatile, or interstitial flow.

Prostate organoid culture under fluorescent microscope

微流控细胞生物学

Prostate Organoid Culture in Microbeads

Microbead-based microfluidics is a powerful technique that generates highly monodispersed picoliter-sized beads into a continuous phase. This method has been successfully adapted to cell culture to encapsulate cells in micron size hydrogel beads. The main advantages are reduced costs related to miniaturization, high reproducibility, and high throughput screening capacities.

微流控系统的要素

Software in Microfluidics

Software is widely defined as a set of computer programs, libraries and data that tell the computer how to work. A user can usually interact with a software through a Graphical User Interface or GUI, whose content is updated by the software’s engine. Our software tools are mostly dedicated to controlling our microfluidics instruments, allowing for remote instruments’ control and sensor’ data logging.

微流控芯片

The Raydrop | A new droplet generation device based on non-embedded co-flow-focusing

After introducing the Raydrop, Adrien Dewandre and his team from ULB demonstrate that this new configuration offers the ability to emulsify any liquid with a wide range of droplet sizes. This universality is demonstrated with experimental results. Modeling is also performed, supporting experimental results, and allowing for the prediction of droplet size and production regime (dripping, jetting regimes).

工业/OEM专业知识

Things you should know when integrating fluidics into your system

The choice of the right method of fluid management can often determinate the success of a project, as well as the overall time and cost of manufacturing. Here is what you need to consider and be aware of when integrating fluidic control into your system.

微流控技术的定义和物理性质

Volumetric Control Technologies

To perform effective experiments in microfluidics, one needs to be aware of the different volumetric control technologies available to use the most suitable way to control microfluidic flows. The present article presents review of the existing techniques.

Here is a list of the main microfluidic flow control technologies.

微流控芯片

What is a microfluidic chip?

We here define microfluidic chips and summarize microfluidic history.

什么是微流控技术？

What is the history of microfluidics?

Microfluidics is both the science which studies the behaviour of fluids through micro-channels, and the technology of systems that process or manipulate small (10-6 to 10-12 litres) amounts of fluids using microminiaturized devices

flow-induced shear stress

微流控细胞生物学

Why is it important to control shear stress in your microfluidic experiments?

Mechanical forces are potent regulators of cellular structures and functions in both health and disease. Of these forces, shear stress is particularly important as it stimulates the release of vasoactive substances and changes gene expression, cell metabolism, and cell morphology.