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Company videos

(We are) RECRUITING for the EXPORT | FLUIGENT INTERVIEW (Le Moci web conference)

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Company videos

2021 International Bicycle Day at Fluigent Microfluidics!

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论文要点

A human BBB (blood brain barrier)-on-chip to assess vascular permeability

A microfluidic model of the human BBB (blood-brain barrier) connected to our Flow-EZ pressure controllers allowing quantitative vascular permeability analyses.

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Agarose microcapsules
实验应用说明

Agarose Microcapsules Synthesis

This application note describes the method for the production of double agarose emulsions using the RayDrop double emulsion chip and Fluigent pressure-based flow controllers. Agarose microcapsules are generated with precise control of particle, core and shell size.

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alginate bead generation monodispercity
实验应用说明

Alginate Microbeads Production

Following is a method for encapsulation of reagent into alginate microbeads with total control of bead formation. A droplet-based microfluidic method is used to precisely control the production of microbeads without the drawbacks of large size distribution that present other methods.

 

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Alginate Microcapsule Synthesis
实验应用说明

Alginate Microcapsule Synthesis

In this application note, we describe the process of producing highly monodisperse alginate microparticles using droplet-based microfluidic methods to control droplet size and core-shell ratio. Different concentrations of alginate have been used to show the versatility and adaptability of the RayDrop in multiple applications.

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digital pcr assay
实验应用说明

Analysis of a commercial surfactant for digital PCR assay

In this application note we are investigating the usability of the commercially available surfactant dSurf for an exemplary digital PCR-assay.

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ARIA sequential injection
Tutorial videos

ARIA Tutorial Episode 1 : How To Plug An ARIA [automated cell perfusion] - Fluigent

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ARIA sequential injection
Tutorial videos

ARIA Tutorial Episode 2 : Connect To Set The Fluidic Path [automated cell perfusion] - Fluigent

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ARIA sequential injection
Tutorial videos

ARIA Tutorial Episode 3 : How To Fill Up The Reservoirs [automated cell perfusion] - Fluigent

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ARIA sequential injection
Tutorial videos

ARIA Tutorial Episode 4 : How To Use ARIA Manually [automated cell perfusion] - Fluigent

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ARIA sequential injection
Tutorial videos

ARIA Tutorial Episode 5 : Perform A Calibration With ARIA [automated cell perfusion] - Fluigent

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ARIA sequential injection
Tutorial videos

ARIA Tutorial Episode 6 : Global Handling Of ARIA [automated cell perfusion] - Fluigent

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ARIA sequential injection
Tutorial videos

ARIA Tutorial Episode 7 : Software Presentation [automated cell perfusion] - Fluigent

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网络研讨会

Automate Cellular Studies with Aria

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webinar fluid delivery fluigent
网络研讨会

Automated fluid delivery for cell and tissue imaging

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automated immunolabeling ibex aria human jejunum
客户案例研究

Automated immunolabeling to perform highly multiplexed tissue imaging with ARIA

Understanding cell-cell interactions and spatial relationships in healthy or diseased organs represents a technical challenge. Single-cell genomics is powerful but lacks spatial context. In this study, automated immunolabeling has been applied to obtain a versatile multiplex optical imaging approach for deep phenotyping and spatial analysis of cells in complex tissues.

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微流控细胞生物学

Biochemical Environment

Cells are constantly exposed to biochemical stimulation from the early embryonic stage to adult life. The spatiotemporal regulation of these signals is essential as it determines cell fate, phenotype, metabolic activity as well as pathological behaviors. The fast response and high stability of Fluigent instruments make them the best solution available on the market to reproduce these complex variations in vitro.

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微流控细胞生物学

Biomechanics

In vivo, most cells are constantly exposed, actively or passively, to mechanical forces. Reproducing these physiological constraints in vitro is essential to induce the right phenotype to cells, finalize their maturation and maintain homeostasis. The wide range of pressure covered by Fluigent products permits one to accurately study biomechanics from molecular level to organ scale.

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实验应用说明

Capillary electrophoresis using microfluidic, electrophoretic, and optic modules

We present here the Lego CE system consisting of available ready-to-use electrophoretic and microfluidic modules, including pressure-based flow controllers. The instrument is coupled with a laser-induced fluorescence detector (LIF) and is demonstrated for separations of labeled oligosaccharides

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webinar fluid delivery fluigent
实验应用说明

Capture and Labeling of Cancer Cells Using Aria

This application note describes the use of the new Fluigent Aria – a software-assisted instrument capable of delivering up to 10 different solutions – for the automation of a complete protocol of capture and labeling of MDA-MB-231 breast cancer cells including surface treatment, injection of antibodies-coated beads, and cell suspension, and immunostaining steps.

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实验应用说明

Cartilage on chip using Fluigent MFCS pressure controller

Elucidating how chondrocytes react to external stimuli (mechanical or chemical) is important to understand processes triggering cartilage diseases like osteoarthritis.  In this application note, we report on the use of Fluigent products to create complex mechanical stimulation patterns on 3D cell culture in a microfluidic platform, or so-called organ-on-a-chip device, with a specific focus on creating a cartilage-on-a-chip model.

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客户案例研究

CEA/CNRS: A flow cell dedicated to imaging in liquid at the nanoscale

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实验应用说明

Cell perfusion with pulse-free flow with one manifold

In this application, one Flow EZ™ pressure channel is connected via a manifold to ten separate vials containing different aqueous solutions. Use of the M-Switch™ and OxyGEN allows the selection of a specific solution directed to a microfluidic device.

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微流控小知识

Choosing the right pressure range

Fluigent offers 10 pressure ranges for its different product lines. Which is the right one for you?

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什么是微流控技术?

Comparison between peristaltic, syringe and pressure pumps for microfluidic applications

This pump selection guide shows the advantages as well as the disadvantages of each method of fluid delivery in Microfluidics so it will help you to choose the proper one for your microflui

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网络研讨会

Concepts of microfluidics

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gut-on-chip caco 2 monolayer
实验应用说明

Development of a human gut-on-chip to assess the effect of shear stress on intestinal functions

Fluid flow and shear stress have previously been reported to promote proper intestinal cell differentiation, formation of villus-like 3D structures and enhanced intestinal barrier function.

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访谈和用户好评

Doing PhD with Fluigent 2021: Research on Honey by Daniel Kraus (Jena, Germany)

One of our PhD students Daniel is doing his postgraduate research on honey at the Leibniz Institute of Photonic Technology. Using the microfluidic set-up, including microfluidic chip and Fluigent pressure pumps

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Ddouble emulsion generation
实验应用说明

Double Emulsion Generation

In this application, we are going to present a robust capillary-based device that allows single-step production of double emulsion with a single device. Different examples of double emulsion (w/o/w and o/w/o) have been generated to show the versatility and adaptability of the Raydrop to multiple applications such as Polymer and Hydrogel microcapsules for drug delivery or for encapsulation of cells for FACS sorting as well.

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网络研讨会

Double emulsion production made easy: a reliable device

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Raydrop Double Emulsion Device
Tutorial videos

DOUBLE EMULSION PRODUCTION: RayDrop Method - Fluigent

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dropseq method
实验应用说明

Drop-Seq method

The Drop-Seq protocol, is a high throughput method that enables the sequencing of the mRNA from a large number of cells. With this method it is possible to create a gene expression map of the cell, or even distinguish cell populations within a tissue!

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实验应用说明

Droplet and particle manipulation using electrophoretic flow control

Many microfluidic applications are developed based on concepts relying on electrodes, exploiting the electrical properties of samples to sort or separate them, generate electro-osmotic flows, manipulate particles, perform electrochemical detection etc. One example of this is electronic paper, where the electrophoretic displacement of titanium dioxide particles in a dark hydrocarbon oil solution allows the formation of a black and white pattern with a high resolution.

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基于压强的微流控技术的优势

Droplet generation using syringe pumps and pressure-based flow controllers

Droplet production using microfluidic systems was implemented for applications where monodispersity is of high importance. Syringe pumps are commonly used for generating droplets in microfluidic experiments, but can show limited flow control.. An alternative to syringe pumps are pressure-based flow controllers. 

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Product presentation videos

DROPLET STARTER package - Make DROPLETS within minutes!

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白皮书

Droplet-based Microfluidics – White Paper

Complete and in-depth overview on droplet microfluidics.

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网络研讨会

Drug encapsulation in biocompatible microparticles for drug delivery

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实验应用说明

E. Coli Culture in Droplets Using dSURF Fluorosurfactant 

In this application note, discover how using microfluidics allows one to encapsulate single or multiple cells into tiny droplets of pL volume which are generated at a rate of approximately one thousand per second.

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cartilage on chip platform
论文要点

Emulating the chondrocyte microenvironment using multi-directional mechanical stimulation in a cartilage-on-chip

A cartilage-on-chip platform coupled to Fluigent pressure controllers to generate compressive or multi-directional mechanical stimulations to study how mechanical stimulation of the chondrocytes can help to reproduce the native articular cartilage microenvironment.

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基于压强的微流控技术的优势

Extended capabilities of pressure driven flow for microfluidics applications

Microfluidic experiment often require a high level of control of fluids to permits reproducible results and the success of different application. To this purpose Pressure based flow controller are well suited for a various number of microlfuidic application when high stability is required such as cell perfusion and shear stress control or in droplet production when monodispersity is of high importance.

It also allows to generate complex flow profil such as sequential injection with aortic profil or when real time flow rate measurement is needed, a parameter that other fluid handling system can't offer.

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网络研讨会

Fast Electrical Impedance Spectroscopy for Characterization and Counting

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微流控系统的要素

Flow control and measurement

The main flow control solutions can be divided in three sections: pressure based solutions (such as the Flow EZ™), volume displacement (such as syringe or peristaltic pumps) and passive techniques. All of these control techniques have different advantages. In order to make the best choice, it is important to consider the following elements.

  • The flow rate or the pressure range you need.
  • How quickly you need to set or change the flow rate.
  • How stable you need the flow rate to be.
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微流控技术的定义和物理性质

Flow Control Technologies

To perform effective experiments in microfluidics, one needs to master the different flow control technologies available to use the most suitable way to control microfluidic flows. This article aims at presenting a short review of the existing techniques.

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flow ez microfluidics flow controller
Tutorial videos

Flow EZ Tutorial Episode 1 : Getting started - Fluigent

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flow ez microfluidics flow controller
Tutorial videos

Flow EZ Tutorial Episode 2 : Insert in my setup - Fluigent

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flow ez microfluidics flow controller
Tutorial videos

Flow EZ Tutorials Episode 3 : Add a Flow EZ - Fluigent

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flow ez microfluidics flow controller
Tutorial videos

Flow EZ Tutorials Episode 4 : Apply a pressure order - Fluigent

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flow ez microfluidics flow controller
Tutorial videos

Flow EZ Tutorials Episode 5: Disconnect a Flow EZ - Fluigent

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flow ez microfluidics flow controller
Tutorial videos

Flow EZ Tutorials Episode 6: Add my FLOW UNIT - Fluigent

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flow ez microfluidics flow controller
Tutorial videos

Flow EZ Tutorials Episode 7: Use the P=0 button - Fluigent

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flow ez microfluidics flow controller
Tutorial videos

Flow EZ Tutorials Episode 8: Switch it off - Fluigent

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实验应用说明

Fluid recirculation for cell perfusion with reduced shear stress

Many microfluidic applications require expensive solutions to be injected at a controlled flow-rate into a microfluidic system, such as cell cultures, PCR processes, cell injections or simulation of blood capillaries with a controlled minimal mechanical stress.

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Company videos

Fluigent CELEBRATES its 10 years-old - A JOURNEY to INNOVATION

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专业知识回顾

Funded research program participation

Many national and internationally funded projects bring together science and innovation. These projects cover a large number of subjects. Fluigent takes part in several European and French funded programs to provide expertise and resources in microfluidics and related applications.

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实验应用说明

Generation of microcapsules with a UV-crosslinked polymer

Using the double emulsion Raydrop, controlled double emulsification is achieved by dripping or jetting the core fluid into an immiscible shell fluid, which is then encapsulated by the third fluid. In this note, capsules are formed by consolidating shell phase of the resulting double emulsions by uv-crosslinking of monomers and photoinitiator used as shell phase.

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网络研讨会

Give a new breath to your microfluidic experiments with OxyGEN

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访谈和用户好评

Hans-Knöll-Institut, New Antibiotics, Cultivation in Droplets

This project focuses on how Fluigent's customers use microfluidics to achieve outstanding results in their technology experiments.

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微流控技术中的液滴和微粒制备

High Throughput Single Cell Analysis

Individual cell heterogeneity within a population has invalidated historic classification methods based on macroscopic considerations and given rise to new evaluation techniques based on single cell transcriptional signature. In this context, thanks to high throughput screening capacities, easy fluid handling and reduced costs related to device miniaturization, microfluidics has emerged as a powerful tool for single cell analysis.

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实验应用说明

High-throughput cell DNA screening using digital PCR

The use of water-in-oil droplets in microfluidics in high-throughput screening is rapidly gaining acceptance. The main application areas currently involve screening cells as well as genetic material for various mutations or activity. Thanks to droplet microfluidic, Michael Ryckelynck and his team are able to isolate single DNA molecules and analyze the enzymes and proteins resulting from their expression.

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微流控技术中的液滴和微粒制备

How to ask cells what proteins they produce?

The conventional way to isolate cells is by using Fluorescence Activation (FACS) to sort them into barcoded well plates (96 wells, 384 or 1536). This step is quite fast with the FACS currently available, but segregating thousands of cells into plates will result in a long process of pipetting. Droplet microfluidics allows for fast and low volume compartmentalization up to a thousand drops produced per second

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Microfluidic chip
微流控芯片

How to choose a microfluidic chip

With this review, we give you some advices on how to select the right microfluidic chip for your application.

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实验应用说明

How to Evaluate Cell Proliferation Using Pressure as a Tool

This application presents a simple method to monitor cell proliferation in microfluidic chips in real time. This is demonstrated experimentally using a custom microfluidic chip. Cell morphology was studied under flowing and static culture condition

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微流控细胞生物学

How to reproduce active biomimetic stimulation in vitro?

Organ-on-a-chip (OOC) technology has paved the way for investigating the impact of mechanical strain in cell biology research by reproducing key aspects of an in vivo cellular microenvironment. Combining microfluidics and microfabrication enables one to reproduce mechanical forces experienced by living tissues at the cell scale.

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webinar spatial transcriptomics
网络研讨会

How to turn your fluorescence microscope into a spatial omics platform

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实验应用说明

Impedance spectroscopy for characterization and counting

We present in this application note our Electrical Impedance Spectroscopy Platform (or EISP) consisting of microfluidic flow controllers from Fluigent to maintain precise flow control, a chip from Micronit Microtechnologies B. V to localize impedance measurements, and a lock-in amplifier from Zurich Instruments to perform impedance measurements. We demonstrate the system efficiency by determining the size of micrometer beads and by measuring the generation rate water-in-oil droplets.

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资助类研究项目的参与

INDEX | H2020 European project

INDEX: Integrated nanoparticle isolation and detection system for complete on-chip analysis of exosomes The aim of the European Union’s Horizon H2020 project, INDEX is to isolate and characterize exosomes available in body fluids through development and integration of novel technological breakthroughs. 

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访谈和用户好评

Interview with Benoit Scheid from Secoya

Benoit Scheid, Senior Researcher, FNRS (National Foundation for Scientific Research).

Professor at the “Université Libre de Bruxelles”, teaching microfluidics to bioengineers and biomedical engineers.

Research activities focusing on process-orientated microfluidics.

Chairman of the Secoya board and scientific advisor.

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oem flow controller component performance
工业/OEM专业知识

Key reliability indicators for OEM components to ensure long-term performance of your flow control system

In this short blog, we review the most important aspects to consider when selecting fluid handling OEM components.

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网络研讨会

LineUp Series, the new generation of microfluidic controllers

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实验应用说明

Liposome Nanoparticles Synthesis

Liposomes were discovered in the 1960s. These hollow nanoparticles are phospholipid vesicles consisting of at least one lipid bilayer. This bilayer is usually composed of amphiphilicphospholipids that have a hydrophilic phosphate head and a hydrophobic tail consisting of two fatty acid chains. This structural feature has facilitated liposomes’ applications, including their use as artificial cell membranes, carriers for drug delivery systems, encapsulating agents forfood ingredients, and analytical tools.

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网络研讨会

Master the production of Double Emulsions

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Product presentation videos

Meet OxyGEN : AUTOMATION and REAL TIME control software - Fluigent

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实验应用说明

Microbiome culture in droplet using dsurf surfactant

The performance of three commonly used surfactants are compared at three different concentrations. The droplet stability over time and the droplet occupation rate is determined by encapsulating a microbial community derived from human skin.

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nozzle for bubble generation
实验应用说明

Microbubbles formation using the RayDrop

Microfluidic devices are used for Bubble generation at micron-scale, yielding high monodispersity and well-controlled size. We investigated how parameters such as the geometry of the nozzle and the continuous-phase flow rate affects the microbubble formation process.

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微流控芯片

Microfabrication of Microfluidic Chips: Materials and Methods 

We discuss the main materials used for microfluidic chips, and their related production methods. In addition, we give you advice on which material one should use depending on the application.

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微流控芯片

Microfluidic chips: key applications

We discuss some applications where microfluidics achieves results that would be very challenging to obtain when using conventional methods.

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Chitosan microcapsule
实验应用说明

Microfluidic Chitosan Microcapsules Production

In this Application Note, chitosan-shell/oily-core microcapsules are generated using the Raydrop double emulsion chip, and Fluigent pressure-based flow controllers. The influence of the fluidic parameters on the size and the release from the oil across the shell are studied and presented.

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微流控技术中的液滴和微粒制备

Microfluidic Droplet Production Method

Droplet microfluidics is a powerful tool which consists of generating and manipulating micron sized monodispersed droplets. A Microfluidic based droplet has many diverse and varied applications such as particle synthesis and physicochemical analysis .A good control of droplet production can also make single-cell analysis, or drug testing possible. In this review we are presenting all microfluidic methods that can be used to produce droplets.

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微流控技术的定义和物理性质

Microfluidic Flow Control Technologies: Strengths and Weaknesses

To perform effective experiments in microfluidics, one needs to master the different flow control technologies available to use the most suitable way to control microfluidic flows. This article aims at presenting a short review of the existing techniques and their strengths and weaknesses.

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microfluidic flow sensing products
微流控系统的要素

Microfluidic Flow Sensing Technologies, A Review

Flow rate measurement is needed for many microfluidic applications, including microfluidic droplet generation, cell culture under flow, and organs on chip. Several technologies aim to provide accurate real time flow measurement to meet this need. In this review, the different existing microfluidic flow sensing solutions for low-flow liquids are described, with their advantages and current limits, as progress is still ongoing in this field.

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基于压强的微流控技术的优势

Microfluidic instrument responsiveness

 In microfluidics, response time is the duration between a command and the the start of flow (the first reaction of the microfluidic pump). When using a pressure controller, flow will start once a sufficient pressure rise occurs in the fluidic reservoir. The electrical and mechanical response times of the hardware components such as valves need to be as low as possible in order to decrease the response time. 

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基于压强的微流控技术的优势

Microfluidic Instrument Stability

In microfluidic instrumentation, stability refers to the ability of an instrument to maintain a certain physical property at a constant value, while rejecting any perturbations in the environment. It is a very important parameter, as even small variations in physical quantities during microfluidic experiments can dramatically change the results. A stable instrument ensures that results are repeatable and reproducible.

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微流控技术的定义和物理性质

Microfluidic Resistance

Microfluidic flows are characterized by the prevalence of the viscous effects compared to inertia. From a physics point of view, this behavior is pointed out by a low Reynolds number indicating laminar flow. It leads to a drastic simplification of the complex Navier-Stokes equations describing fluid displacement.

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微流控系统的要素

Microfluidic Tubing

Several parameters must be taken into consideration in order to choose the tubing:

  • Materials
  • Tubing dimensions such as the outer diameter“OD”,  the inner diameter “ID” and the length  “L”.  For example the inner diameter plays a significant role in the resistivity brought by the tubing: the smaller it is, the more resistant the tubing will be...
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Product presentation videos

MICROFLUIDIC VALVE AUTOMATION: How to make it easy [SWITCH EZ] - Fluigent

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Product presentation videos

Microfluidic Valves : SMART CONTROL and AUTOMATION of your fluidic path - Fluigent

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微流控技术的定义和物理性质

Microfluidic volume definitions

Most of the time, it is useful to know the total volume of one’s fluidic circuit. Volumes in microfluidics can be different from other areas. The chip has its own volume, tubing has an internal volume. Fittings like unions, adapters or tees can have an enclosed volume that will contribute to the total volume of a system. This is referred in specification sheets as “internal volume”.

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白皮书

Microfluidic white paper - A guide to Organs-on-Chips technology

Discover the concepts of OOC engineering and their applications in medical sciences.

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什么是微流控技术?

Microfluidics definitions and advantages

Microfluidics is the science of manipulating and controlling fluids, usually in the range of microliters (10-6) to picoliters (10-12), in networks of channels with dimensions from tens to hundreds of micrometers. 

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Raydrop Single Emulsion Chip Secoya Fluigent
Tutorial videos

Microfluidics for Alginate Microbeads Production - Fluigent

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实验应用说明

Microfluidics for Transmission Electron Microscopy: Characterization of Copper Electrodeposition

This application presents the Liquid-phase Transmission Electron Microscopy (LPTEM) technique, which integrates integrates liquid flow capabilities within microfabricated liquid cells, providing the means to study different processes in solution with sub-nanometer spatial resolution and sub-microsecond temporal resolution. Using this technique, it is now possible to visually study topics ranging from material science to life science.

 

 

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专业知识回顾

Microfluidics for vaccines research and development

Microfluidic methods can be used to improve vaccine research and development. Microfluidic techniques are already used to develop adjuvants, perform virus identification/diagnostics, or drug micro and nanoencapsulation.

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Expertise videos

MICROFLUIDICS in DROPLET DIGITAL PCR

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论文要点

Microfluidics Technology for the Design and Formulation of Nanoparticles

Engineered Nanoparticles (NPs) are becoming essential to enhance drug delivery systems (DDSs) for both biomedical and pharmaceutical applications. Controlling the delivery of molecules of interest, increases their concentration in a desired location and lead to improved drug efficacy as well as minor adverse effects. NPs including lipid-based and polymeric NPs have been used over the years as nanocarriers of various bioactive molecules.

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微流控细胞生物学

Micropipette aspiration of cells and tissues

Micropipette aspiration is a powerful non-invasive technique to evaluate how biomechanical properties of single cells or tissue govern cell shape, cell response to mechanic stimuli, transition from nontumorigenic to tumorigenic state or morphogenesis

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modular pressure and flow control platform
视频

Modular pressure & flow control platform - unboxing & setup

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实验应用说明

Multiple emulsion droplet generation

How to encapsulate multiple aqueous droplets (called “core”) into a single oil shell.

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资助类研究项目的参与

MYOCHIP | H2020 European project

Myochip: Building a 3D innervated and irrigated muscle on a chip

The aim of the European Union’s Horizon H2020 project, Myochip  is to build a muscle on chip. Myochip gathers 4 partners from all around the world: The institute of molecular medicine (Portugal), The University of Edinburg (Scotland), and Institut Curie (France).

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网络研讨会

New micropipette aspiration package

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网络研讨会

New Platform for complex emulsion generation – Fluigent

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oem customised automated drug screening system
客户案例研究

OEM Case Study: Microfluidic Drug Screening

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实验应用说明

Oil in Water Emulsions

In this application note, we present droplet generation data obtained using decane in Water, a system that demonstrates the expected behavior of most hydrocarbons in Water. We demonstrate the ability of Fluigent equipment coupled with Raydrop microfluidic devices to generate high-quality emulsions with controlled droplet sizes and with high throughput.

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网络研讨会

Organ on a chip Towards the next generation of cell culture platforms

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Tutorial videos

OxyGEN Automation Protocols: Creation and Editing

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Tutorial videos

OxyGEN Automations Protocols: Container Blocks for Loops and Conditions

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Tutorial videos

OxyGEN for Live Control of FLUIGENT Microlfuidic Devices: Getting Started

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Tutorial videos

OxyGEN: Advanced Live Control of a Microfluidic Set-Up

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微流控细胞生物学

Passive mechanical stimulation induced by laminar and pulsatile shear stress

Indirect mechanical forces and shear stress are integral parts of the cellular microenvironment. Reproducing these mechanical forces and shear stress is critical to capture the physiology of living tissues. Three types of flows are typically generated for producing shear stress : laminar, pulsatile, or interstitial flow.

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实验应用说明

Peristaltic pump vs pressure-based microfluidic flow control systems for Organ on-chip applications

Microfluidic cell culture has significant advantages over macroscopic culture in flasks, Petri dishes, and well-plates. To demonstrate the importance of flow stability in vascular models, endothelial cells seeded in microfluidic chips were perfused either using a peristaltic pump or pressure-based flow controllers. 

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实验应用说明

PLGA microcapsules synthesis

In this Application Note, PLGA shell/aqueous core microcapsules are obtained using the Secoya Raydrop Double emulsion. The influence of the fluidic parameters on the microcapsule size and release from the oil across the shell are explored in this application note.

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实验应用说明

PLGA Microparticles Synthesis

When PLGA is used as an active pharmaceutical ingredient carrier it is important to produce highly monodispersed particles for drug release reproducibility. The most common production process of PLGA particles is solvent based and can involve hazardous solutions. Ethyl acetate is preferred as it shows better biocompatibility than other conventional solvent such as dichloromethane.

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plga nanoparticle synthesis acetone and water pva
实验应用说明

PLGA nanoparticle synthesis using 3D microfluidic hydrodynamic focusing

In this Application Note, PLGA nanoparticles with high monodispersity are generated using the Raydrop single emulsion developed and manufactured by Secoya, and Fluigent pressure-based flow controllers. The ability to synthesise PLGA nanoparticles in a more controllable and reproducible way creates possibilities to tailor surface properties and increase fields of application.

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Fluigent P SWITCH MICROFLUIDIC VALVE
Product presentation videos

PNEUMATIC VALVE CONTROLLER: How to do multiple and parallel pressurization [P-SWITCH] - Fluigent

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liquid crystal double emulsions webinar
网络研讨会

Polymerization of microfluidics-produced liquid crystal double emulsions for making wavelength and polarization-selective retroreflectors

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实验应用说明

Pressure predictions for lab-on-a-chip operations using a microfluidic network solver and Fluigent PX

DownloadDirect download
实验应用说明

Production of water-in-oil emulsions using a droplet generator chip

In this application note, we demonstrate droplet generation using the Fluigent microfluidic system including pressure pumps, chemicals, tubing and a Droplet Generator Chip obtained from our partner microfluidic ChipShop.

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Prostate organoid culture under fluorescent microscope
微流控细胞生物学

Prostate Organoid Culture in Microbeads

Microbead-based microfluidics is a powerful technique that generates highly monodispersed picoliter-sized beads into a continuous phase. This method has been successfully adapted to cell culture to encapsulate cells in micron size hydrogel beads. The main advantages are reduced costs related to miniaturization, high reproducibility, and high throughput screening capacities.

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Raydrop Single Emulsion Chip Secoya Fluigent
Tutorial videos

RayDrop Tutorial episode 1: How To Fill the Raydrop ? - Fluigent

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Raydrop Single Emulsion Chip Secoya Fluigent
Tutorial videos

RayDrop Tutorial episode 2: How To Clean the Nozzle (1/3) ? - Fluigent

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Raydrop Single Emulsion Chip Secoya Fluigent
Tutorial videos

RayDrop Tutorial episode 3: How To Clean the Nozzle (2/3) - Fluigent

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Raydrop Single Emulsion Chip Secoya Fluigent
Tutorial videos

RayDrop Tutorial episode 4: How To Clean the Nozzle (3/3) - Fluigent

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Raydrop Single Emulsion Chip Secoya Fluigent
Tutorial videos

RayDrop Tutorial episode 5: How To Clean the Chamber - Fluigent

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Raydrop Single Emulsion Chip Secoya Fluigent
Tutorial videos

RayDrop Tutorial episode 6: How To Change Nozzle ? - Fluigent

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网络研讨会

Raydrop, a universal droplet generator based on a non-embedded co-flow-focusing

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Chitosan microcapsule
网络研讨会

Robust technology for double emulsion production and focus on microcapsule/microparticle synthesis

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实验应用说明

Single cell sorter microfluidic platform

Passive cell or particle sorting using a dedicated package. To demonstrate the separation of particle mixtures, a solution containing 7.5 µm and 15 µm diameter polystyrene particles labeled with FITC, and TRITC fluorophores respectively was used. The particle streams were viewed and captured separately using appropriate filter cubes.

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微流控系统的要素

Software in Microfluidics

Software is widely defined as a set of computer programs, libraries and data that tell the computer how to work. A user can usually interact with a software through a Graphical User Interface or GUI, whose content is updated by the software’s engine. Our software tools are mostly dedicated to controlling our microfluidics instruments, allowing for remote instruments’ control and sensor’ data logging.

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客户案例研究

Success story of SEED Biosciences : Single-cell injection and impedance analysis

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ARIA sequential injection
访谈和用户好评

Testimonials Aria, automated sequential perfusion

I got to test ARIA injection system in my research project in a collaboration with Fluigent. More precisely, ARIA injection system helped me automatize the capture process and immunostaining of breast cancer cells under a very precise and controlled flow rate...

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访谈和用户好评

Testimonials dSurf

dSURF improved our droplet stability and reliability of droplet formation under control of Fluigent systems. We performed good quality dPCR and droplet-based micro-cultivation of microbials...

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flow ez microfluidics flow controller
访谈和用户好评

Testimonials Flow EZ

We use the pressure-based pumps from Fluigent for experiments that require swift responsiveness when manipulating fluids, and fine tuning at low flow rates. We use the Fluigent systems during the fabrication and running of the microfluidic chips...

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访谈和用户好评

Testimonials MFCS Series

The Fluigent MFCS controller allowed us a quick and hassle-free development of our solution focusing on our real application! This has also been accelerated by the dedicated and professional support we got from the amazing Fluigent team...

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Push-Pull, Pressure & Vacuum Based Flow Controller
访谈和用户好评

Testimonials Push-Pull

The Fluigent LineUp series, including the new push-pull pump, enables precise and highly controlled aspiration and respiration of liquids. The set-up allows us to further advance our research in both continuous flow and droplet microfluidics...

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访谈和用户好评

Testimonials Raydrop

The RayDrop microfluidic chip from Fluigent gave us the best results with better control over the process

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Epigenomics Ram Laboratory RNA Sequencing
客户案例研究

The Hebrew University: Encapsulation and culture in 3D hydrogels for Single cell sequencing 

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客户案例研究

The Micro/Nano Bioelectronics and Biosensors (MBIOS) from Tianjin University

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Product presentation videos

The NEW GENERATION of PRESSURE CONTROLLERS : LineUp series

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微流控芯片

The Raydrop | A new droplet generation device based on non-embedded co-flow-focusing

After introducing the Raydrop, Adrien  Dewandre and his team from ULB  demonstrate that this new configuration offers the ability to emulsify any liquid with a wide range of droplet sizes. This universality is demonstrated with experimental results. Modeling is also performed, supporting experimental results, and allowing for the prediction of droplet size and production regime (dripping, jetting regimes).

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工业/OEM专业知识

Things you should know when integrating fluidics into your system

The choice of the right method of fluid management can often determinate the success of a project, as well as the overall time and cost of manufacturing. Here is what you need to consider and be aware of when integrating fluidic control into your system.

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tubing and fitting kit for alginate production station
Expertise videos

TUBING and FITTINGS in MICROFLUIDICS - Fluigent

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客户案例研究

University of Cambridge: Giant unilamellar vesicle production and testing

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客户案例研究

University of Maryland: A soft robotic hand with integrated fluidic circuitry that can play Nintendo

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实验应用说明

UV-Crosslinking of Microparticles

In this short note, we present a Fluigent microfluidic solution for polymer microparticle synthesis with high monodispersity (2% CV). The microfluidic system allows for inline particle generation, spacing, and polymerization by UV light.

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微流控技术的定义和物理性质

Volumetric Control Technologies

To perform effective experiments in microfluidics, one needs to be aware of the different volumetric control technologies available to use the most suitable way to control microfluidic flows. The present article presents review of the existing techniques.

Here is a list of the main microfluidic flow control technologies.

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实验应用说明

Water in Fluorocarbon Oil Emulsions

In this application note, we present droplet generation data obtained using Fluigent’s own fluorocarbon oil formulation – dSURF to demonstrate the ability of Fluigent equipment coupled with Raydrop microfluidic devices to generate high-quality emulsions with controlled droplet sizes and with high throughput.

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实验应用说明

Water in Oil Emulsions

In this application note, we present droplet generation data obtained using one of the most widely used water in oil emulsion systems – water in decane. We demonstrate the ability of Fluigent equipment coupled with RayDrop microfluidic devices to generate high-quality emulsions with controlled droplet sizes and with high throughput.

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微流控芯片

What is a microfluidic chip?

We here define microfluidic chips and summarize microfluidic history.

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什么是微流控技术?

What is the history of microfluidics?

Microfluidics is both the science which studies the behaviour of fluids through micro-channels, and the technology of systems that process or manipulate small (10-6 to 10-12 litres) amounts of fluids using microminiaturized devices 

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flow-induced shear stress
微流控细胞生物学

Why is it important to control shear stress in your microfluidic experiments?

Mechanical forces are potent regulators of cellular structures and functions in both health and disease. Of these forces, shear stress is particularly important as it stimulates the release of vasoactive substances and changes gene expression, cell metabolism, and cell morphology.

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