Raydrop Single Emulsion

[1DPRD01]

    Single emulsion and droplet generator

    The RayDrop is a microfluidic droplet generator composed of three main parts fully removable: two inserts on each side and a center section containing a nozzle and an outlet capillary. There are four standard microfluidic connections, two on the box for the continuous phase, and one on each insert for the dispersed phase entry and the collecting emulsion outlet.

     

    The RayDrop specific design allows for multiple liquid type emulsification, within the same device without any coating needed. This allows to cover single and complex emulsions.

    This allows to cover many application from encapsulation of active pharmaceutical ingredient in both polymer microparticle to cell and fragrance encapsulation in hydrogel microbeads such as:

    • PLGA microparticle
    • Alginate microbeads
    • Liposome Nanoparticle
    Main benefits
    • droplet monodispersity
      Reproducible

      High monodispersity (CV < 2%)

    • ICO_BFT size
      Flexible

      Multiple applications

    • Easy

      Coating-free

    Features

    A flexible device

    Droplet size from 20 µm to 450 µm diameter. Frequency of up to 10 000 Hz. Water-in-oil (w/o) and oil-in-water (o/w) droplets.

    A unique device

    One device for multiple applications.

    Surface coating-free

    As opposed to standard droplet devices, the Raydrop does not require any surface coating steps.

    Easy to use

    Easy-to-clean exchangeable nozzle. Easy microscope visualization.

    Developed and manufactured by Secoya

    Description

    The RayDrop works as a co-flow focusing principle. The nozzle and outlet capillary are aligned in continuous phase chamber, the dispersed phase comes through the nozzle to create the microparticles into the continuous phase and exit by the outlet insert.

    The RayDrop is available in three standard configuration (Nozzle size-Extraction capillary size), in order to target a broader droplet size range:

    • 30µm-150µm
    • 60µm-300µm
    • 90µm-450µm

    As all cappilaries from the RayDrop are easily excheangable it’s easy to change either the nozzle capillary or the collecting cappilary with another nozzle or cappilary size.
    It can also be tuned to target different droplet size by changing cappilaries size and nozzle configuration:


    Small droplet configuration:
    30µm-60µm

    Small droplet configuration: 30µm-70µm-90µm

    This two nozzle configuration allow to decrease double emulsion size down to 15µm droplet size.


    “My team has started using the Fluigent microfluidic kit for making polymer based particles. We tried different microfluidic systems in the past but unfortunately they were not suitable for our application. The main issue was chip blockage which rendered the process quite laborious.

    The RayDrop microfluidic chip from Fluigent gave us the best results with a better control over the process.

    I have to mention the excellent technical support from Fluigent, their team visited my lab 3 times to deliver training and help to optimize the process”

    Dr Omar QUTACHISenior Lecturer in PharmaceuticsHealth and Life Sciences Montfort University , The Gateway, Leicester

    Specifications

    Device characteristicsCo-Flow focusing design
    Droplet typewater in oil and oil in water
    Droplet size15 to 450 µm
    Generation rate10 000 Hz (measured for the smallest double emulsion size) – can go higher under specific conditions
    Nozzle typeMethalcrylate resin
    Glass
    Nozzle size30 µm ID
    60 µm ID
    90 µm ID
    Inlet and outlet capillary150 µm ID
    300 µm ID
    450 µm ID
    External dimensionsL*l*h = 60 mm * 40 mm * 13 mm
    Weight205
    Operating pressure0 – 5 bar
    Burst pressure10 bar
    Wetted material: continuous phasePEEK, FEP, glass, stainless steel, polyimide, Kalrez (seal), methalcrylate resin or glass (nozzle)
    Wetted laterial: dispersed phase PEEK, FEP, glass, methalcrylate resin or glass (nozzle)

    PNProduct NameCapillary sizesNozzle type
    1DPRD01Complete Raydrop30µm-150µmMethacrylate Resin
    ORDRPSE-60-300Complete Raydrop60µm-300µmMethacrylate Resin
    ORDRPSE-90-450Complete Raydrop90µm-450µmMethacrylate Resin
    O-SE-RDRPC04-EUPComplete Raydrop30µm-60µmMethacrylate Resin
    O-SE-RDRPC05-EUPComplete Raydrop30µm-90µmMethacrylate Resin
    O-SE-RDRPC06-EUPComplete Raydrop60µm-90µmMethacrylate Resin
    ORDRPSNO-30Simple nozzle insert30µmMethacrylate Resin
    ORDRPSNO-60Simple nozzle insert60µmMethacrylate Resin
    ORDRPSNO-90Simple nozzle insert90µmMethacrylate Resin
    ORDRPCOL-150Collecting capillary insert150µmGlass
    O-SP-CCI300-COCollecting capillary insert300µmGlass
    O-SP-CCI450-COCollecting capillary insert450µmGlass
    O-SE-RDRPC01G-EUPComplete Raydrop30µm-150µmGlass
    O-SE-RDRPC02G-EUPComplete Raydrop60µm-300µmGlass
    O-SE-RDRPC03G-EUPComplete Raydrop90µm-450µmGlass
    O-SE-GSNO30-EUPSimple nozzle insert30µmGlass
    O-SE-GSNO60-EUPSimple nozzle insert60µmGlass
    O-SE-GSNO90-EUPSimple nozzle insert90µmGlass

    Double emulsion production: Raydrop Microfluidics for Alginate Microbeads Production


    How to fill the Raydrop?

    Which solvent is compatible with the Raydrop?

    As chemicals, can be used ethylacetate, ethanol or IPA. (The nozzle material is not compatible at long-term use with dichloromethane)


    Do I need to filter my solutions?

    Yes, we recommend filtering all solutions before starting your experiment. The presence of dust can block the nozzle.


    There is an air bubble in my device. How can I get rid of it?

    Open the top plugs of the RayDrop.

    Return to the RayDrop filling state.

    Refer to the application note protocol.


    There is a fluid leak coming our from the sides of the Raydrop. Is it a common behavior?

    Leaks can sometimes appear if the fluidic connectors are not tightened enough.

    Check that all the connectors are attached firmly.

    (Be careful not to over tighten or your tubing may be blocked by pinching)


    All my connectors are tigh but there is still water flowing out of the Raydrop window. Is this a common behavior?

    If the RayDrop has been manipulated (for cleaning or maintenance) the four screws might not be tightened enough.

    Use an allen key to tighten.


    How do I clean the Raydrop after my experiment?

    It is important to clean the RayDrop after each experiment in order to prevent clogging.

    Refer to the good practice guide for the detailed procedure.


    How can I avoid air bubble infiltration?

    Make sure that all tubing is well flushed before connecting the RayDrop.