High control over bead size
Alginate Bead Generation Pack
[O-SE-ALG-PCK]A system for producing monodisperse alginate beads
The Fluigent Alginate bead generation pack is a robust and complete system for producing outstanding monodispersed Alginate microparticles. The generation pack is flexible, allowing the alginate microparticle sizes to change in hundreds of milliseconds without interrupting the production.
The generation pack is powered by Fluigent’s LineUP microfluidic pumps and the RayDrop device, a breakthrough technology for high-quality particles and beads production.
- Flexible
- Reproducible
High monodispersity (2%)
- Robust
Continuous production
Alginate microparticles are used in a wide range of therapeutic applications including encapsulation, therapeutics delivery, tissue engineering, regenerative medicine and in vitro cell culture. Some of the striking characteristics of alginate beads are their biocompatible nature, easy gelation and availability of open functional groups with ease of manipulation to synthesize alginate derivatives with advanced desirable properties. Alginate microbeads are recommended due to their nontoxicity and biodegradability, and also because they are formed from naturally occurring polysaccharides and can be made from a rapid preparation method.
The application note demonstrates that alginate beads can be successfully produced using our setup with precise control of droplet size.
Moreover, it’s possible to use different concentrations of alginate due to the great flexibility of our system, which allows users to change the nozzle in case of working with high viscosity liquids. Changing capillary size can be easily done by the user to target different droplet sizes.
Due to the precision and versatility of the Alginate bead generation pack, it’s possible to produce particles for various biological applications: encapsulation, drug delivery, stem cell culture, and tissue engineering scaffolds.
Features of the Alginate Beads Pack
Complete system
This packages includes all the components necessary to start generating alginate droplets.
Engineered solution
The package was built with pressure controllers, microfluidic chips, and valves that are customizable in terms of droplet size and generation rate.
Dedicated protocol
A protocol is available to assist you in setting up and starting your alginate microparticles generation experiments.
Customization possible
We can adapt the alginate beads generation pack to fulfil your needs (droplet size, generation rate).
Related applications
Main products of the Alginate bead generation pack
Microfluidics for Alginate Microbeads Production – Tutorial video
Specifications
Beads production
Dispersed phase | Alginate + Calcium + EDTA |
Alginate concentration | 0.5%, 1%, 1.5%, 2% |
Continuous phase | dSurf |
Particle size range | 50 – 250 µm |
Production frequency | Up to 5 000 Hz |
Monodispersity | 2% |
Flow Control
Pumps | Fluigent Flow EZ (2 000 mbar) |
Flow sensors | Fluigent Flow Unit (M) |
Automated valves | Fluigent 2-SWITCH |
Imaging (optional)
Microscope | Fluigent Digital high-speed microscope |
OxyGEN
Control in real-time, protocol automation, data record and export |
ver. 2.2.0.0 or more recent |
Software Development Kit
Custom software application |
ver. 22.2.0.0 or more recent |
Imaging
OptoViewer Software |
Extrusion method | Fluigent microfluidic method | |
Particle size distribution | up to 50% | ~ 2% |
Reproducibility | low | High |
Particle size control | no | Precise |
Continuous (in line) production | no | yes |
Expertise & resources
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Microfluidics White Papers Droplet-based Microfluidics Read more
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Fluigent Products Datasheets Raydrop single emulsion datasheet Download
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Expert Reviews: Basics of Microfluidics The Raydrop | A new droplet generation device based on non-embedded co-flow-focusing Read more
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Microfluidic Application Notes Alginate Microbeads Production Read more
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Fluigent Products Datasheets FLOW UNIT Datasheet Download
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Fluigent products manual Alginate user manual Download
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Fluigent Products Datasheets Datasheet Pack Alginate Download
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Fluigent Products Datasheets Flow EZ™ Datasheet Download
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