BE-Transflow

Perform Air Liquid Interface (ALI) experiments on a 2D or 3D culture with automatic culture medium replacement for systems such as epithelial cultures, toxicity tests, absorption test and much more.

Crosstalk studies (prototype): Explore the cross-talk of two cultures in an automated and simple way using the BE-Transflow option that connects two wells in the same channel. Most common application are indirect toxicity studies. This chip is a prototype and is available at a higher price than the normal BE-Transflow.

BE-Transflow has been designed with ALI in mind. Its an ideal chip for ALI culture and coculture research (skin, cornea, gut, lung). However, its simplicity to use makes it also a very useful tool for any coculture research and crosstalk experiments.

Epidermis/dermis coculture: Recreation of the epidermis and dermis in the well of the BE-Transflow device. In the upper part of the image and marked in blue are the nuclei of the keratinocytes (HEKa) forming the epidermis. The HEKa cells were cultured for 21 days in the ALI to create a mature epidermis. Underneath the epidermis we can see the fibroblasts in red embedded in a collagen matrix, mimicking the dermis. The scale bar marks 100 µm.