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The most versatile cell culture chip

[OOC-TRNS-07]

    BE-Transflow

    BE-Transflow is Beonchip’s most versatile cell culture platform. It allows the study of complex culture configurations by joining a culture well with a microfluidic channel via a porous membrane. This is the optimal device for Air Liquid Interface (ALI) culture, endothelium/epithelium barrier and crosstalk studies.

    Ask for a quoteBuy online
    be transflow microfluidic chip
    Main benefits
    • Easy to use

      Simple fitting & connections

    • Biocompatible
      Biomimetic environment

      2D and 3D culture

    • Sterile

      Dedicated to cell biology

    Features

    Easy to use

    Be-Transflow is compatible with any type of optical microscopy and its slide format has been chosen for easy handling under a microscope.

    Easy to connect

    Be-Transflow is compatible with all Fluigent pressure-based flow controllers

    No unspecific absorption

    Unlike in other PDMS devices, Be -Doubleflow is made of lipophobic thermoplastic materials and does not present unspecific drug absorption issues. It allows immunohistochemistry with fluorescent detection

    Cell recovery

    The cell cultures used in the Be-Transflow can be easily recover for further experimentation.

    Easy to implement

    Be-transflow wells are positioned in standard positions of a 96 well plate for its use in automated microscopes. In addition, their volume is identical to the one of those wells in order to ease the transition to the transflow device.

    Related applications

    • Microfluidics for Organ-on-chip Cell culture

      Discover

    Culture models

    Perform Air Liquid Interface (ALI) experiments on a 2D or 3D culture with automatic culture medium replacement for systems such as epithelial cultures, toxicity tests, absorption test and much more.

    Create the perfect endothelium endothelium-epithelium barrier: Use the upper well for the 2D or 3D epithelial culture and seed the endothelial cells in the perfusable channel underneath. Most common applications are Blood Brain Barrieer (BBB), gut, skin, lung and many more.

    Crosstalk studies (prototype): Explore the cross-talk of two cultures in an automated and simple way using the BE-Transflow option that connects two wells in the same channel. Most common application are indirect toxicity studies. This chip is a prototype and is available at a higher price than the normal BE-Transflow.

    Applications

    BE-Transflow has been designed with ALI in mind. Its an ideal chip for ALI culture and coculture research (skin, cornea, gut, lung). However, its simplicity to use makes it also a very useful tool for any coculture research and crosstalk experiments.

    Epidermis/dermis coculture: Recreation of the epidermis and dermis in the well of the BE-Transflow device. In the upper part of the image and marked in blue are the nuclei of the keratinocytes (HEKa) forming the epidermis. The HEKa cells were cultured for 21 days in the ALI to create a mature epidermis. Underneath the epidermis we can see the fibroblasts in red embedded in a collagen matrix, mimicking the dermis. The scale bar marks 100 µm.

    Bone on chip: BEOnchip participates in the Eurostars project BONAFIDE. This project is devoted to the production of a human bone-on-chip platform that will simulate bone growth, resorption and remodeling for future evaluation of (anti-osteoporosis) drugs and biomaterials (e.g. bone cements).

    The culture well represents the catabolic side (bone resorption) where the osteoclasts rest in contact with a bonelike scaffold. The lower channel represents the anabolic side of the bone model. This channel is seeded with osteocytes and osteoblast that form a 2D tissue.

    This tissue is mechanically stimulated by the controlled laminar flux applied in the channel, mimicking the physiological environment of the bone. The bone remodeling process is automatically analyzed using image analysis software and biomarker analysis.

    omi organ models development

    Specifications

    PERFORMANCE

    HeightWidthLengthTotal volume
    Channel375 µm1.5 mm45 mm44 µL
    Well6 mm5.7 mm5.7 mm195 µL
    Inlet/outlet7 mmUNF 1/4″ – 28 UNF 1/4″ – 28 130 µL
    Medium reservoir7 mm3.6 mm8.8 mm185 µL
    Membrane pore size1 µm 1 µm 1 µm 1 µm

    Getting Started

    Cell culture

    https://www.youtube.com/watch?v=fTzss-34fOE

    Expertise & resources

    • Technical datasheets BE-Transflow datasheet Download
    • Expert Reviews: Basics of Microfluidics How to reproduce active biomimetic stimulation in vitro? Read more
    • Expert Reviews: Basics of Microfluidics Passive and active mechanical stimulation in microfluidic systems  Read more
    • Expert Reviews: Basics of Microfluidics Mimicking in-vivo environments: biochemical and biomechanical stimulation  Read more
    • Expert Reviews: Basics of Microfluidics Microfluidic chips: key applications Read more

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