Workshop - Double emulsion production compatible with fluorescence-activated cell sorting (FACS) for cell sorting application

Overview

Analyzing the cellular behavior at a single-cell level is of critical importance to better understand the heterogeneity of cellular response such as protein secretion or enzymatic activity.

To this purpose Fluorescence-Activated Cell Sorting (FACS) appears as a convenient tool to indicate the production of a molecule of interest. On the other side microfluidics technology and more exactly encapsulation of cells in aqueous droplets allow to study at single cell level the cellular response such as protein secretion or enzymatic.

The combination of these two different technologies offers lot of potential to study single cell expression however a single water in-oil (W/O) emulsion is actually not well suited to FACS sorting.

Indeed, FACS sorting requires that the particles are suspended in a aqueous phase to efficiently sort the particles of interests by applying an electric charged. To avoid these limitations, the W/O droplet can be encapsulated in a 2nd aqueous layer to form a water-in-oil-in-water (W/O/W) droplet, which is compatible with FACS.

In this workshop we will present you how to encapsulate biological materials such as cell inside double emulsions, in an easy and robust way, which are totally compatible with FACS sorting

Agenda

During this workshop, you will :
• Get aware about new emulsification technology
• Learn how to encapsulation cell in Double emulsion using microfluidics
• Understand how to sort cells in double emulsion using FACS
• Live Demonstration of the generation of Double emulsion
• Interactive Q&A session to discuss your application needs (20 mins)