Microfluidic chip for 2D and 3D cultures

Explore the crosstalk in the endothelium epithelium barrier under flow in both channels of the device, mimicking a more physiological environment for the study of different organs such as kidney, liver, gut, or heart, among others.

Research the effect of circulating particles such as circulating tumor cells, immune system cells, bacteria, fungi, viruses, and many more in single cell culture, or in a complex coculture.

BE-Doubleflow allows endothelium/epithelium barrier coculture where a hypoxia environment is needed or when flux plays a role also in the epithelium culture (Kidney, liver, heart, lung, gut…). In addition, its two perfusable channels offer a perfect environment for studying the effect of circulating particles (bacteria, immune response, circulating tumor cells).

Example applications

Kidney on chip: The coculture of HPTC cells and vascular endothelial cells clearly improves the performance of the HPTCs and increases the maintenance of the renal epithelia in vitro. Using BE-Doubleflow it is possible to coculture both types of cells simultaneously, circulating urine on the renal epithelium culture and blood-like medium in the vascular culture.