Cell perfusion package
The perfect set for cell perfusion
Fluigent has joined with BEOnchip to offer complete solutions for organ-on-chip applications combining innovative, robust, and easy-to-use chips with high-performance instruments.
- Designed for cell biology
- Easy to use
Cell seeding in 3D hydrogel is a delicate manipulation. Hydrogels are fragile and soft. They can easily fracture or detach from the chip if the flow is unstable.
Manual cell seeding is not recommended as the discontinuous flow results in heterogeneous cell seeding and can crack the hydrogel.
The manual mode of the Flow EZ is perfect to circumvent these limitations as it allows to implement small flow rate increments while keeping an eye on the microscope.
Stable & complex flow patterns
Fluigent’s products have unprecedented performances in terms of stability and responsiveness.
High stability is particularly useful for applications with constant shear stress like vascular perfusion.
High responsiveness is essential to reproduce complex flow patterns like aortic pressure variations. For complex flow pattern, the orders can be set in flow or pressure variations.
Once the parameters are set and optimized, protocol automation is the key to saving time, limiting contamination, and decreasing variability.
For any protocol, valves, pressure, and flow controller from Fluigent can be assembled to automate the protocol using user-friendly software (OxyGEN).
We can adapt the package to fulfill your needs of specific flow rates, or pressure
Main products of the package
Pressure-based flow controller
Bidirectional flow sensor
Easy-to-use cell culture
Microfluidic chip for 2D and 3D cultures
The most versatile cell culture chip
Designed for the application of electrochemical gradients to 3D cell cultures
Air-tight metal cap
Cell culture under flow
Cultivating cells under flow perfusion has two main advantages: constant medium renewal and reproduction of mechanical strain in vitro.
To grow cells under perfusion, we recommend using the BE-Flow chip.
Cells are constantly exposed to biochemical stimulation from the early embryonic stage to adult life. The spatiotemporal regulation of these signals is essential as it determines cell fate, phenotype, metabolic activity as well as pathological behaviors. Biochemical stimulation is also central in oncology as it drives the migration and expansion of malign tumors.
To study chemotaxis in 3D hydrogel we recommend using BE-GRADIENT.
Co-culture under perfusion
Co-culture under perfusion is essential for various applications:
- to fully differentiate given cell types, coculture with another cell type is required
- in cosmetics or drug testing to reproduce interfaces link skin, lung or gut and monitor the uptake of active molecules
- to reproduce liquid-liquid or air-liquid interfaces.
The BE-Doubleflow chip best fits these applications as a porous membrane separates the central chamber into two channels.
Transwell under flow
Transwells are classic devices widely used in standard cell culture to co-culture cell types, reproduce 2D-2D interfaces, 3D-2D interfaces, study cell invasion, assess monolayer permeability or active molecule uptake.
For this application, we recommend the BE-Transflow
Cell culture with regulated Shear Stress
Mechanical forces are potent regulators of cellular structures and functions in both health and disease. As a result of their unique location, endothelial cells experience several mechanical forces.
Of these forces, shear stress is particularly important as it stimulates the release
of vasoactive substances and changes gene expression, cell metabolism, and cell morphology.
To grow cells under regulated shear stress, we recommend the BE-FLOW
|LineUp Flow EZ pressure controller|
|LineUp LINK Module (software control)|
|BEOnChip microfluidic chip|
|Fluigent connectors and tubing kit|
|Control in real-time, protocol automation, data record and export|
|ver. 126.96.36.199 or more recent|
Software Development Kit
|Custom software application|
|ver. 188.8.131.52 or more recent|
Connect the chip to the microfluidic system
Switching from static culture to fluid perfusion
Expertise & resources
Expertise reviews Why is it important to control shear stress in your microfluidic experiments? Read more
Application notes Development of a human gut-on-chip to assess the effect of shear stress on intestinal functions Read more
Webinars Organ on a chip Towards the next generation of cell culture platforms Read more
Application notes Peristaltic pump vs pressure-based microfluidic flow control systems for Organ on-chip applications Read more
Technical datasheets BE-Gradient datasheet Download
Technical datasheets BE-Transflow datasheet Download
Technical datasheets BE-flow datasheet Download
Technical datasheets BE-DoubleFlow Standard datasheet Download
Expertise reviews How to reproduce active biomimetic stimulation in vitro? Read more
Expertise reviews Passive mechanical stimulation induced by laminar and pulsatile shear stress Read more
Technical datasheets FLOW UNIT Datasheet Download
Expertise reviews Biomechanics Read more
Expertise reviews Biochemical Environment Read more
Technical datasheets Flow EZ™ Datasheet Download