Cell perfusion package

    The perfect set for cell perfusion

    Fluigent has joined with BEOnchip to offer complete solutions for organ-on-chip applications combining innovative, robust, and easy-to-use chips with high-performance instruments.

    Main benefits
    • Biocompatible
      Designed for cell biology

      Contamination-free, sterile

    • Easy to use

      Simple connections


    Optimized seeding

    Cell seeding in 3D hydrogel is a delicate manipulation. Hydrogels are fragile and soft. They can easily fracture or detach from the chip if the flow is unstable.

    Manual cell seeding is not recommended as the discontinuous flow results in heterogeneous cell seeding and can crack the hydrogel.

    The manual mode of the Flow EZ is perfect to circumvent these limitations as it allows to implement small flow rate increments while keeping an eye on the microscope.

    Stable & complex flow patterns 

    Fluigent’s products have unprecedented performances in terms of stability and responsiveness.

    High stability is particularly useful for applications with constant shear stress like vascular perfusion.

    High responsiveness is essential to reproduce complex flow patterns like aortic pressure variations. For complex flow pattern, the orders can be set in flow or pressure variations.

    Protocol automation

    Once the parameters are set and optimized, protocol automation is the key to saving time, limiting contamination, and decreasing variability.

    For any protocol, valves, pressure, and flow controller from Fluigent can be assembled to automate the protocol using user-friendly software (OxyGEN).

    Customization possible

    We can adapt the package to fulfill your needs of specific flow rates, or pressure

    Main products of the package


    Cell culture under flow

    Cultivating cells under flow perfusion has two main advantages: constant medium renewal and reproduction of mechanical strain in vitro.
    To grow cells under perfusion, we recommend using the BE-Flow chip.

    Read more


    Cells are constantly exposed to biochemical stimulation from the early embryonic stage to adult life. The spatiotemporal regulation of these signals is essential as it determines cell fate, phenotype, metabolic activity as well as pathological behaviors. Biochemical stimulation is also central in oncology as it drives the migration and expansion of malign tumors.
    To study chemotaxis in 3D hydrogel we recommend using BE-GRADIENT.

    Read more

    Co-culture under perfusion

    Co-culture under perfusion is essential for various applications:

    • to fully differentiate given cell types, coculture with another cell type is required
    • in cosmetics or drug testing to reproduce interfaces link skin, lung or gut and monitor the uptake of active molecules
    • to reproduce liquid-liquid or air-liquid interfaces.

    The BE-Doubleflow chip best fits these applications as a porous membrane separates the central chamber into two channels.

    Read more

    Transwell under flow

    Transwells are classic devices widely used in standard cell culture to co-culture cell types, reproduce 2D-2D interfaces, 3D-2D interfaces, study cell invasion, assess monolayer permeability or active molecule uptake.

    For this application, we recommend the BE-Transflow

    Read more

    flow-induced shear stress

    Cell culture with regulated Shear Stress

    Mechanical forces are potent regulators of cellular structures and functions in both health and disease. As a result of their unique location, endothelial cells experience several mechanical forces.

    Of these forces, shear stress is particularly important as it stimulates the release
    of vasoactive substances and changes gene expression, cell metabolism, and cell morphology.

    To grow cells under regulated shear stress, we recommend the BE-FLOW

    Read more


    LineUp Flow EZ pressure controller
    LineUp LINK Module (software control)
    BEOnChip microfluidic chip
    Fluigent connectors and tubing kit


    Control in real-time, protocol automation, data record and export
    ver. or more recent

    See the offer

    Software Development Kit

    Custom software application
    ver. or more recent

    See the offer

    Connect the chip to the microfluidic system

    Switching from static culture to fluid perfusion