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Automated multiplexed imaging platform
An all-in-one system for successful imaging experiments
Fluigent has partnered with Bioptechs, the world leader in micro-environmental control chambers, to offer complete solutions for high-quality automated multiplexed imaging. The system combines a precision flow cell with a high-performance perfusion instrument. This flexible platform allows users to automate complex protocols with multiple fluid delivery for imaging.
- Time saving
Automation to gain productivity
Easily moved between microscopes
Features of Automated Multiplexed Imaging
Preserve the sample
- Temperature controlled flow cell: the flow cell can be cooled down or heated up from 0 – 58 C with less than 0.2 C variance and integrated intelligent feedback loop for external temperature variables
- Controlled perfusion flow rate: avoids abrupt manual injection that can damage both the sample, the reagent, and the microscopy workstation
- Enclosed system: contamination free
Protocol automation drastically reduces variability down to 0,5% between experiments compared to 5,1% intra-operator variability and 8,1% inter-operator variability using a pipette.
Synchronize with microscope
Aria and the FCS Chamber System are equipped with TTL ports to either send or receive TTL signals. TTL communication with a microscope enables users to synchronize perfusion and imaging.
This automated multiplexed imaging platform is a cost-effective alternative to an all-in-one system. In addition, as the platform is flexible and transportable, it can be easily moved between microscopes to perform various sets of experiments, and is highly adaptable to protocols and investigations.
Description of the multiple fluid delivery platform
The automated multiplexed imaging platform is the perfect compromise between manual pipetting and an all-in-one system that is dedicated to one specific application, integrating a microscope, a specific chip type, and a given set of solutions. Any protocol with multiple solution delivery can be automated, saving the scientist both time and reducing variability between experiments compared to manual procedures.
The platform, which integrates our automated sequential injection system, ARIA , is straightforward and ready-to-use. No specific training is required, and no time is lost troubleshooting incidents or manually priming solutions before the experiment as is the case with homemade systems.
The automated multiplexed imaging platform is designed to offer maximum flexibility e in terms of:
- Applications: the instrument and its intuitive software automates any sequence of various function: perfusion of up to 10 solutions, incubation, synchronization with the microscope via TTL signaling, flush tubing
- Chamber design: the flow cell comes with a wide range of gaskets that fit biological samples from 2D cells to thick tissues. It is available in cooled, no-heat, and temperature controlled with an intelligent feedback system to maintain perfect temperature control for long term investigations, and features leak proof flow
- Microscopy: Our automated multiplexed imaging platform is adaptable to any microscope upright or inverted
- Reagents: any type of antibody, rna or dna sequence can be used.
Automation provides a high gain in productivity compared to manual pipetting.
The 20 step immunoassay protocol, which takes 2 days with manual pipetting, is shortened to several hours, increasing the overall productivity from 2 samples a week to 1 sample a day.Automation also significantly reduces operational variability and pipetting errors.
Watch the webinar on the benefits of automation and examples of applications.
Automated fluid delivery for cell and tissue imaging
You wish to gain time, precision and reproducibility with your immunofluorescence assay or any other assay requiring injection of multiple solutions on your sample. Follow our webinar to discover how to interface a flow chamber (or a microfluidic chip) to our automated fluid delivery device ARIA. This will allow you to deliver up to 10 different solutions in a sequential and autonomous manner. In addition, ARIA can be synchronized with any microscope to launch an image acquisition cycle and to resume the perfusion protocol once the imaging cycle has been completed.
This all-in-one workflow facilitates the succession of cycles of injection/incubation time with reagents and image acquisition, a feature particularly well adapted to complex cell and tissue imaging. Here, we will present some examples of applications such as multiplexed tissue imaging, DNA-PAINT or seqFISH as well as cell capture and staining.
00:00 – Reasons & Advantages to use automated sequential fluid deliver
08:30 – Demo video
10:27 – Example of applications:
- 10:33 – Automated multiplexed tissue imaging
- 16:55 – Sequential fluorescence in situ hybridization (SeqFISH)
- 20:14 – Capture and characterization of circulating tumor cells
26:40 – Q&A session
Conference animated by :
Mélanie Chabaud (Fluigent – Application Engineer)
William César (Fuigent – R&D project manager)
Noé Viovy (Fluigent – R&D software developer)
Applications of Automated Multiplexed Imaging
This kit is perfect for any researcher who is looking to automate the labeling process of living cells, fixed tissues, and more.
Applications this system can be directly applied to include but are not limited to::
- Shear stress experiments
- Extended imaging of live 3D cell culture
- Omics applications including but not limited to: proteomics and spatial-omics
- Ratio-metric imaging such as calcium rationing
- Intracellular trafficking
- Any manner of fluorescence in situ labeling, including but not limited to: FISH, seq-FISH, MER-FISH, HCR-FISH
- Toxicity testing
- Drug response studies
Aria comes with an intuitive software that enables to design protocols in few clicks.
Incubation time, flow rate, volume dispensed, are all parameters that can be easily set by the operator for each step of his protocol. Protocols are recorded and can be shared among users. Flow rate and pressure are recorded for each experiment
How to mount the flow cell?
How to connect Aria to the FCS2?
Connect the output of the 2 switch valve to the flow cell using a P702 union
How to plug an Aria
Connect to set the fluidic path
How to fill up the reservoir
How to use Aria manually
Perform a calibration with Aria
Global handling of Aria
Expertise & resources
Microfluidic Application Notes Capture and Labeling of Cancer Cells Using Aria Read more
Microfluidic Webinars Automate Cellular Studies with Aria Read more
Microfluidic Webinars How to turn your fluorescence microscope into a spatial omics platform Read more
Microfluidic Webinars Organ on a chip Towards the next generation of cell culture platforms Read more
User manuals FCS2 Chamber instructions Download
User manuals FCS2 Chamber temperature instructions Download
Technical datasheets Aria datasheet Download
Expert Reviews: Basics of Microfluidics Passive mechanical stimulation induced by laminar and pulsatile shear stress Read more
Expert Reviews: Basics of Microfluidics Biomechanics Read more
Expert Reviews: Basics of Microfluidics Biochemical Environment Read more