Search results for : oxygen

Our latest software for control in real time and automation

Dr. Agarwal’s Physiomimetic Microsystems Laboratory (PML) developed the microfluidic pancreas-on-chip platform that can provide controlled oxygen and perfusion environments for viability and real-time imaging.

Learn how automation in microfluidics enhances precision and reproducibility through real-time monitoring and intelligent feedback control.

We discuss some applications where microfluidics achieves results that would be very challenging to obtain when using conventional methods.

Software is widely defined as a set of computer programs, libraries and data that tell the computer how to work. A user can usually interact with a software through a Graphical User Interface or GUI, whose content is updated by the software’s engine. 

In this review, we explain why microfluidics is the best alternative to enhance organoid viability, promote tissue maturation, and support vascularization. 

Explore best practices in microfluidic perfusion for organ-on-a-chip, live-cell imaging, drug testing, and advanced flow control systems

The use of Fluigent pressure-based flow controllers has been a key factor in achieving microfluidic GUV production using the OLA method, facilitating the production of vesicles with higher monodispersity and size control.

A precise flow control system and micro-cannula injection method were developed to regulate atrial preload and assess cardiac function in zebrafish larvae.

CloneSeq: A highly sensitive single-cell analysis platform for the comprehensive characterization of cells from 3D culture.

New 3D model for cancer investigations based on the mimicking of tumor microenvironment using pressure pump to recreate interstitial flow.

Discover the precision of our microfluidic system: A soft robotic hand with integrated fluidic circuitry that can play Nintendo Mario Bros

Many national and internationally funded projects bring together science and innovation. These projects cover a large number of subjects. Fluigent takes part in several European and French funded programs to provide expertise and resources in microfluidics and related applications.

This review discusses the importance of perfusion and pressure control in Organ-on-Chip (OOC) technologies. It compares various pump and flow control systems, outlines key factors to consider when choosing the right technology, and showcases real-world examples of OOC applications using Fluigent devices.

Discover microfluidic interfaced capillary electrophoresis for automated, precise analysis and monitoring of biomolecular interactions and drug delivery.

Discover how to successfully design and implement Organ-on-Chip (OoC) models — from flow control to physiological design, avoid common pitfalls and boost reproducibility.

Recirculation in microfluidics plays a central role in both life-science and chemical applications. In cell biology and organ-on-chip (OOC), recirculation enables long-term medium perfusion, maintains physiological conditions, reduces reagent consumption, and supports stable shear stress. In chemical microreactors, recirculation can improve conversion, permit multi-pass reactions, and reduce waste.

This application presents the Liquid-phase Transmission Electron Microscopy (LPTEM) technique, which integrates integrates liquid flow capabilities within microfabricated liquid cells, providing the means to study different processes in solution with sub-nanometer spatial resolution and sub-microsecond temporal resolution. Using this technique, it is now possible to visually study topics ranging from material science to life science.

With this review, we give you some advices on how to select the right microfluidic chip for your application.

To ensure the reliability and the sealing integrity of devices, rigorous quality control testing is essential. This application note discusses the importance of leak detection on a micro scale and various methodologies to perform it.

This paper highlight evaluates the flow uncertainty for Fluigent MSFC-EZ and syringe pumps and their impact on optical imaging.

The Drop-Seq protocol, is a high throughput method that enables the sequencing of the mRNA from a large number of cells. With this method it is possible to create a gene expression map of the cell, or even distinguish cell populations within a tissue!

Method for the microfluidic study of bacterial biofilms using pressure pump coupled with UV-C emission to target biofilm formation in an area of interest.

In this application note, we present droplet generation data obtained using one of the most widely used water in oil emulsion systems – water in decane. We demonstrate the ability of Fluigent equipment coupled with RayDrop microfluidic devices to generate high-quality emulsions with controlled droplet sizes and with high throughput.

In this application note, discover how using microfluidics allows one to encapsulate single or multiple cells into tiny droplets of pL volume which are generated at a rate of approximately one thousand per second.

This application presents a simple method to monitor cell proliferation in microfluidic chips in real time. This is demonstrated experimentally using a custom microfluidic chip. Cell morphology was studied under flowing and static culture condition

This study is the first to achieve vascularization of kidney organoids in HUVEC co-culture conditions using a microfluidic organ-on-chip. This model offers valuable insights into kidney organoid vascularization and is a promising tool for studying kidney development and drug testing.

Bimodal microscopy measures red blood cell mechanosensitive channel activation and mechanical properties during micropipette aspiration using Flow EZ.

Discover the concepts of OOC engineering and their applications in medical sciences.

In this application note, we present the RayDrop™ (designed and developed by Secoya Tehcnologies), coupled with Fluigent’s Flow EZ controllers, to encapsulate iron oxide nanoparticles in monodisperse microcapsules.

Droplet microfluidics enables precise liquid control for drug discovery, diagnostics, and materials. Follow these 10 tips to master stability, flow, and encapsulation.

Practical workflows, common organ models, and the evolving regulatory landscape. 

We demonstrate the RayDrop’s ability to produce PLGA microparticles in the range of 1 to 10 µm, for encapsulation and drug delivery.

The importance of flow stability in microfluidic systems is demonstrated by performing cell culture using peristaltic pumps or pressure-based flow controllers.  

This customer case study introduces an innovative platform, including Fluigent’s pressure controllers and valves, for ocular drug delivery monitoring.

Cells are constantly exposed to biochemical stimulation from the early embryonic stage to adult life. The spatiotemporal regulation of these signals is essential as it determines cell fate, phenotype, metabolic activity as well as pathological behaviors. The fast response and high stability of Fluigent instruments make them the best solution available on the market to reproduce these complex variations in vitro.

Pressure driven flow is crucial in many microfluidic applications as it allows for precise and efficient control of fluid movement at the microscopic scale.

In this application note, we report on the combination of Fluigent PX with a microfluidic network solver for highly accurate predicted pressures and millisecond response times. 

Liposome preparation using a complete microfluidic setup (pressure controller) to encapsulate amiodarone, a promising drug in ovarian cancer.

This paper assesses a new temperature measurement method at the micro-scale at high throughput.

Our advanced platform for long-term recirculation of fluid, ideal for Organ-on-a-Chip studies and the reproduction of relevant physiological conditions, providing deeper insights into in-vivo phenomena.

In this application note, we describe the process of producing highly monodisperse alginate microparticles using droplet-based microfluidic methods to control droplet size and core-shell ratio. Different concentrations of alginate have been used to show the versatility and adaptability of the RayDrop in multiple applications.

In this Application Note, chitosan-shell/oily-core microcapsules are generated using the Raydrop double emulsion chip, and Fluigent pressure-based flow controllers. The influence of the fluidic parameters on the size and the release from the oil across the shell are studied and presented.

This application note describes the method for the production of double agarose emulsions using the RayDrop double emulsion chip and Fluigent pressure-based flow controllers. Agarose microcapsules are generated with precise control of particle, core and shell size.

In this Application Note, PLGA shell/aqueous core microcapsules are obtained using the Secoya Raydrop Double emulsion. The influence of the fluidic parameters on the microcapsule size and release from the oil across the shell are explored in this application note.

In this application note, we associate the double emulsion microfluidic platform with FACS sorting technology to sort out small droplets encapsulating fluorescent e-coli bacteria.