Simple connections
Immunostaining Package
O-MULTILABEL-PCKA ready-to-use package for sequential injection of multiple fluids for cell or tissue staining
The Immunostaining Package provides stable and automated delivery of up to 10 different fluids to a single cell culture chamber.
- Easy
- Designed for cell biology
Contamination-free, Sterile environment
- Compact
Inject up to 10 different samples
- Versatility
Possibility to heat or agitate reservoir
Features
Complete system
All components necessary for immunostaining included in the package.
Versatility
This package can be used for diverse applications, and with any microfluidic chip.
The reservoirs can be easily manipulated, to be heated or agitated for example.
Stable flow rate & short switching time
Fluigent’s products allow for a very stable delivery and a short switching time for the best results in immunostaining.
Dedicated protocol
Build customized, time-based protocols to save time and reduce variability with our user-friendly software OxyGEN.
Our software allows easy and complete protocol automation.
Large volume sample
Ten injection reservoirs allow for the manipulation of large volumes of samples.
A large range of pressure and flow rates are available thanks to our pressure based flow controller.
Sequential injections
Up to 10 sequentially selected liquids be delivered to a chip by the M-SWITCH. The samples at the outlet of the chip may also be sorted using a 2-SWITCH either into a collection tube or to waste.
User-friendly software (OxyGEN) is available for each step.
Related applications
- Immunostaining
Microfluidics for Cell Analysis
DiscoverMicrofluidics for Organ-on-chip Cell culture
DiscoverMicrofluidics for Cell Biology
Discover
- Chip preparation : Chip coating, cell loading, cell incubation
Main products of the Immunostaining package
Flow EZ™, Microfluidic flow controller
Pressure-based flow controller
LINK
Software control
P-CAP series
Air-tight metal cap
Bidirectional Microfluidic Flow Sensor
FLOW UNIT/ FLOW UNIT +
2-SWITCH™ Microfluidic Sampling Valve
3-port/2-way valve
M-SWITCH™, Microfluidic Bidirectional Valve
11-port/10-position valve
OxyGEN
Automation & Control
Specifications
PRODUCTS
Our Immunostaining Package contains the following :
LineUp Flow EZ pressure controller (1000 mbar) (x1) |
LineUp LINK Module (software control) (x1) |
FLOW UNIT M (x1) |
2-SWITCH (x1) |
SWITCH EZ (x1) |
P-CAP series 15 mL (x12) |
M-SWITCH (x1) |
10 Position Pressure Manifold (x1) |
FLUID HANDLING SYSTEM
Product | Part number |
1*Flow EZ | LU-FEZ-1000 |
1*2-SWITCH | 2SW002 |
1*SWITCH EZ | ELUSEZ |
1*LINK module | LU-LNK-0002 |
1*M-SWITCH | ESSMSW003 |
RESERVOIRS
Product | Part number |
12*15 mL Pcap with 15 mL Falcon tube | P-CAP15-HP |
1*10 Position Pressure Manifold | CTQ-MANI |
FLOW METERS
Product | Part number |
1*Flow unit M | FLU-M-D |
TUBING
Product | Part number |
Tubing and connection Kit P-CAP 15mL FEP tubing with an ID of 500 microns | CTQ-KIT-PC15 |
1*Tubing and connection Kit 2-SWITCH | CTQ-KIT-2SW2 |
1*Tubing and connection Kit Flow UNIT M | CTQ-KIT-LQ |
1*Tubing and connection Kit M-SWITCH | CTQ-KIT-ESSMSW003 |
OxyGEN
Real-time control, automated protocol, data record and export |
ver. 2.1.0.0 or more recent |
Software Development Kit
Custom software application |
ver. 21.0.0.0 or more recent |
“We have been using Fluigent’s M-SWITCH, amongst other accessories, including Flow EZ system, flow controllers, for the past 5 years. We are pleased with its performance, especially the programmability and automation of fluid flow that relieves the user of the need to be present next to the experiments that run for several hours. We found the software interface to be simple to use and could count on the team’s assistance for friendly recommendations and support.”
Sivashankar Krishnamoorthy – Luxembourg Institute of Science and Technology (LIST)
Expertise & resources
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Technical datasheets Immunostaining Package Datasheet Download
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Microfluidic Webinars Automated fluid delivery for cell and tissue imaging Read more
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Microfluidic Application Notes Development of a human gut-on-chip to assess the effect of shear stress on intestinal functions Read more
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Expert Reviews: Basics of Microfluidics How to choose a microfluidic chip Read more
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White Papers Microfluidic white paper – An exploration of Microfluidic technology and fluid handling Read more
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Technical datasheets M-SWITCH™ Datasheet Download
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User manuals Easy Switch Solutions User Manual Download
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Microfluidic Application Notes Capture and Labeling of Cancer Cells Using Aria Read more
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Microfluidic Webinars Concepts of microfluidics Read more
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Microfluidic Webinars Automate Cellular Studies with Aria Read more
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Microfluidic Webinars Organ on a chip Towards the next generation of cell culture platforms Read more
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Microfluidic Webinars LineUp Series, the new generation of microfluidic controllers Read more
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Microfluidics case studies CEA/CNRS: A flow cell dedicated to imaging in liquid at the nanoscale Read more
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Microfluidic Application Notes Cell perfusion with pulse-free flow with one manifold Read more
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User manuals SWITCH EZ User Manual Download
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Technical datasheets SWITCH EZ Datasheet Download
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Expert Reviews: Basics of Microfluidics Extended capabilities of pressure driven flow for microfluidics applications Read more
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Expert Reviews: Basics of Microfluidics What is a microfluidic chip? Read more
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Expert Reviews: Basics of Microfluidics Microfluidic chips: key applications Read more
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Expert Reviews: Basics of Microfluidics Comparison between peristaltic, syringe and pressure pumps for microfluidic applications Read more
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Technical datasheets Flow EZ™ Datasheet Download
For more integration
For a completely integrated, ready-to-use, and intuitive system to perform sequential perfusion and immunostaining experiments, Aria is the designated product.
Aria is our automated solution for cellular perfusion or timed injection protocols. Aria allows one to automate delivery up to 10 different solutions into a chamber or microfluidic chip by following user defined protocols.
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