Microfluidic Application Notes

Fluigent’s broad range of solutions for nanofluidic and microfluidic application note, both in scientific research and the industrial/OEM sector, offer greater control, automation, precision, and ease of use. Whether your application features droplets, cell biology, particle studies, or other research areas, we have the expertise and knowledge to provide the most cost effective and technically advanced solutions for your fluid control needs. Our first class flow control technology integrates in many biomedical research fields as it provides stable flow with high control and precision, which is already documented in various research articles, application notes, or congresses. This page features numerous microfluidic application notes that can help you with your setup and experiments. You will find tutorials, accompanied by an extensive and varied literature search, explaining the step by step process of how to perform the experiment optimally and efficiently, as well as the results obtained and the conclusion drawn from the experiment. Other applications may be developed with the product's brand provided by Fluigent. Have you published a paper using Fluigent products? Contact us at contact@fluigent.com and we will reference it on our site!

Ensuring Quality Control in Micro-Scale Devices by Innovative Leak Detection Method

To ensure the reliability and the sealing integrity of devices, rigorous quality control testing is essential. This application note discusses the importance of leak detection on a micro scale and various methodologies to perform it.

Caco2 cells after 7 days of culture medium recirculation with Omi

Microfluidic Application Notes

Omi, automated organ-on-chip platform, the Gut-on-chip model development

Microfluidics for gut-on-chip model enable precise control over the microenvironment, mimicking the complex physiological conditions of the human gut. These systems allow for the co-culture of various cell types under dynamic flow conditions, promoting tissue differentiation and function. This technology offers valuable insights into gut physiology, disease modeling, and drug testing by replicating the intricate interactions between the gut epithelium, microbiota, and immune cells.

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Microfluidic Application Notes
Endothelial Cell Culture Under Shear Stress Using Omi

This Application Note provides a protocol for creating a HUVEC monolayer in the BeonChip BeFlow microfluidic chip.

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Microfluidic Application Notes
Creating Microcapsules With PEGDA Hydrogel

Monodispersed PEGDA hydrogels microparticles and microcapsules for enhanced stability and controlled release.

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Microfluidic Application Notes
Automating calcium imaging in neural cells with Fluigent’s Aria

Explore automated calcium imaging with Fluigent's Aria, enhancing precision and efficiency in neuronal activity analysis. Discover our streamlined protocol.

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Microfluidic Application Notes
1-10 microns PLGA microsphere production using the RayDrop

We demonstrate the RayDrop’s ability to produce PLGA microparticles in the range of 1 to 10 µm, for encapsulation and drug delivery.

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Microfluidic Application Notes
Automating Neuronal Cell Immunofluorescence in Microfluidic Chips

Streamline immunolabeling of neuron cells across as many as 4 microfluidic chips with an automated perfusion system.

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Microfluidic Application Notes
Pressure predictions for lab-on-chip operations using a microfluidic network solver and Fluigent PX

In this application note, we report on the combination of Fluigent PX with a microfluidic network solver for highly accurate predicted pressures and millisecond response times.

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Microfluidic Application Notes
A quick and efficient double encapsulation method for FACS-based droplet sorting

In this application note, we associate the double emulsion microfluidic platform with FACS sorting technology to sort out small droplets encapsulating fluorescent e-coli bacteria.

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Microfluidic Application Notes
Long-term fluid recirculation system for Organ-on-a-Chip applications

Our advanced platform for long-term recirculation of fluid, ideal for Organ-on-a-Chip studies and the reproduction of relevant physiological conditions, providing deeper insights into in-vivo phenomena.

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Microfluidic Application Notes
Automated Immunofluorescence using Aria

Cutting-edge Automated Immunofluorescence protocol that leverages the capabilities of the automated sequential injection system

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Microfluidic Application Notes
What is the best method for Microencapsulation of Bacteria and Yeast in Small Double Emulsions?

In this application note, we describe the encapsulation method of the yeast strain S.Cerevisiae CEN.PK 113-7D and the bacterial strain L.cremoris MG1363_GFP in double emulsions of 42 µm size using the Cell Encapsulation Platform.

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Microfluidic Application Notes
Encapsulation of Cells In Small Double Emulsions

In this application note, we have demonstrated that the Cell Encapsulation Platform can encapsulate cells in w/o/w double emulsions with precise droplet size control. Human Adult Peripheral Blood Mononuclear Cells (PBMC) were encapsulated in a 50 µm monodisperse water-oil-water double emulsion using a Raydrop^TM with a 60-120-60 configuration

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Microfluidic Application Notes
Alginate Microcapsule Synthesis

In this application note, we describe the process of producing highly monodisperse alginate microparticles using droplet-based microfluidic methods to control droplet size and core-shell ratio. Different concentrations of alginate have been used to show the versatility and adaptability of the RayDrop in multiple applications.

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Microfluidic Application Notes
Agarose Microcapsules Synthesis

This application note describes the method for the production of double agarose emulsions using the RayDrop double emulsion chip and Fluigent pressure-based flow controllers. Agarose microcapsules are generated with precise control of particle, core and shell size.

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Microfluidic Application Notes
PLGA nanoparticle synthesis using 3D microfluidic hydrodynamic focusing

In this Application Note, PLGA nanoparticles with high monodispersity are generated using the Raydrop single emulsion developed and manufactured by Secoya, and Fluigent pressure-based flow controllers. The ability to synthesise PLGA nanoparticles in a more controllable and reproducible way creates possibilities to tailor surface properties and increase fields of application.

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Microfluidic Application Notes
Encapsulation of multiple emulsions in a single droplet

In this application note, we describe the encapsulation of multiple aqueous “core” droplets inside a single oil droplet called a “shell”.

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Microfluidic Application Notes
Microbubble formation using the RayDrop

Microfluidic devices are used for Bubble generation at micron-scale, yielding high monodispersity and well-controlled size. We investigated how parameters such as the geometry of the nozzle and the continuous-phase flow rate affects the microbubble formation process.

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Microfluidic Application Notes
Development of a human gut-on-chip to assess the effect of shear stress on intestinal functions

Our gut-on-chip system made of BEOnChip microfluidic chips coupled to pressure controllers offers a way to control and mimic the microenvironment of in vitro cultured intestinal epithelium that is closer to the physiological state.

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Microfluidic Application Notes
PLGA microcapsules synthesis

In this Application Note, PLGA shell/aqueous core microcapsules are obtained using the Secoya Raydrop Double emulsion. The influence of the fluidic parameters on the microcapsule size and release from the oil across the shell are explored in this application note.

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Microfluidic Application Notes
Pressure predictions for lab-on-a-chip operations using a microfluidic network solver and Fluigent PX
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Microfluidic Application Notes
Droplet and particle manipulation using electrophoretic flow control

Many microfluidic applications are developed based on concepts relying on electrodes, exploiting the electrical properties of samples to sort or separate them, generate electro-osmotic flows, manipulate particles, perform electrochemical detection etc. One example of this is electronic paper, where the electrophoretic displacement of titanium dioxide particles in a dark hydrocarbon oil solution allows the formation of a black and white pattern with a high resolution.

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Microfluidic Application Notes
Liposome Nanoparticle Synthesis

In this application note, the Raydrop (developed and manufactured by Secoya) is used to perform Liposome Nanoparticle synthesis.

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Microfluidic Application Notes
High-throughput cell DNA screening using digital PCR

Thanks to droplet microfluidic, Michael Ryckelynck and his team are able to isolate single DNA molecules and analyze the enzymes and proteins resulting from their expression.

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Microfluidic Application Notes
Cartilage-on-a-chip, an example of complex mechanical stimulation using Fluigent’s technology

A new method for 3D chondrocyte culture using a microfluidic platform that allows researchers to create a relevant cartilage model, subject to complex mechanical stimulation.

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Microfluidic Application Notes
PLGA Microparticles Synthesis

PLGA microparticles are of great interest for life science applications. This application note focus on microfluidic PLGA microparticles generation methods.

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Microfluidic Application Notes
E. Coli Culture in Droplets Using dSURF Fluorosurfactant

In this application note, discover how using microfluidics allows one to encapsulate single or multiple cells into tiny droplets of pL volume which are generated at a rate of approximately one thousand per second.

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Microfluidic Application Notes
Droplet Sequencing: Drop-Seq method

The Drop-Seq protocol, is a high throughput method that enables the sequencing of the mRNA from a large number of cells. With this method it is possible to create a gene expression map of the cell, or even distinguish cell populations within a tissue!

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Microfluidic Application Notes
Single cell sorter microfluidic platform

Passive cell or particle sorting using a dedicated package. To demonstrate the separation of particle mixtures, a solution containing 7.5 µm and 15 µm diameter polystyrene particles labeled with FITC, and TRITC fluorophores respectively was used. The particle streams were viewed and captured separately using appropriate filter cubes.

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Microfluidic Application Notes
Microbiome culture in droplet using dsurf surfactant

The performance of three commonly used surfactants are compared at three different concentrations. The droplet stability over time and the droplet occupation rate is determined by encapsulating a microbial community derived from human skin.

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Microfluidic Application Notes
Microfluidic Chitosan Microcapsules Production

In this Application Note, chitosan-shell/oily-core microcapsules are generated using the Raydrop double emulsion chip, and Fluigent pressure-based flow controllers. The influence of the fluidic parameters on the size and the release from the oil across the shell are studied and presented.

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Microfluidic Application Notes
Generating a water emulsion in an oil solution using a droplet generator chip

In this application note, We explain how to generate water emulsions in oil solutions using the Fluigent microfluidic system including pressure pumps, chemicals, tubing and a Droplet Generator PDMS Chip.

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Microfluidic Application Notes
Alginate Microbeads Production

Following is a method for encapsulation of reagent into alginate microbeads with total control of bead formation. A droplet-based microfluidic method is used to precisely control the production of microbeads without the drawbacks of large size distribution that present other methods.

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Microfluidic Application Notes
Double Emulsion Generation

In this application note we have demonstrated how we can perform different type of double emulsion generation with a single device.

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Microfluidic Application Notes
Assess Cell Proliferation Using Pressure as a Tool

This application presents a simple method to monitor cell proliferation in microfluidic chips in real time. This is demonstrated experimentally using a custom microfluidic chip. Cell morphology was studied under flowing and static culture condition

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Microfluidic Application Notes
Capillary electrophoresis using microfluidic, electrophoretic, and optic modules

Here we present here the Lego CE system consisting of available ready-to-use electrophoretic and microfluidic modules, including pressure-based flow controllers. The instrument is coupled with a laser-induced fluorescence detector (LIF) and is demonstrated for separations of labeled oligosaccharides

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Microfluidic Application Notes
Microfluidics for Transmission Electron Microscopy: Characterization of Copper Electrodeposition

This application presents the Liquid-phase Transmission Electron Microscopy (LPTEM) technique, which integrates integrates liquid flow capabilities within microfabricated liquid cells, providing the means to study different processes in solution with sub-nanometer spatial resolution and sub-microsecond temporal resolution. Using this technique, it is now possible to visually study topics ranging from material science to life science.

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Microfluidic Application Notes
Analysis of a commercial surfactant for digital PCR assay

In this application note we are investigating the usability of the commercially available surfactant dSurf for an exemplary digital PCR-assay.

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Microfluidic Application Notes
UV-Crosslinking of Microparticles

In this short note, we present a Fluigent microfluidic solution for polymer microparticle synthesis with high monodispersity (2% CV). The microfluidic system allows for inline particle generation, spacing, and polymerization by UV light.

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Microfluidic Application Notes
UV-Crosslinking of Microcapsules

In this application note, we describe the UV-crosslinking of microcapsules process, in which microcapsules are formed by consolidating shell phase of the resulting double emulsions by uv-crosslinking of monomers and photoinitiator used as shell phase.

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Microfluidic Application Notes
Water in Fluorocarbon Oil Emulsions

Achieve reproducible, high-quality water in fluorocarbon oil emulsions with cutting-edge microfluidic devices. Ideal for Drop-Seq and RNA-Seq studies.

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Microfluidic Application Notes
Water in Oil Emulsions

In this application note, we present droplet generation data obtained using one of the most widely used water in oil emulsion systems – water in decane. We demonstrate the ability of Fluigent equipment coupled with RayDrop microfluidic devices to generate high-quality emulsions with controlled droplet sizes and with high throughput.

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Microfluidic Application Notes
Oil in Water Emulsions

In this application note, we present droplet generation data obtained using decane in Water, a system that demonstrates the expected behavior of most hydrocarbons in Water. We demonstrate the ability of Fluigent equipment coupled with Raydrop microfluidic devices to generate high-quality emulsions with controlled droplet sizes and with high throughput.

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Microfluidic Application Notes
Impedance Measurement of Microbeads

We present in this application note our Electrical Impedance Spectroscopy Platform (or EISP) consisting of microfluidic flow controllers from Fluigent to maintain precise flow control, a chip from Micronit Microtechnologies B. V to localize impedance measurements, and a lock-in amplifier from Zurich Instruments to perform impedance measurements. We demonstrate the system efficiency by determining the size of micrometer beads and by measuring the generation rate water-in-oil droplets.

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Microfluidic Application Notes
Peristaltic Pump vs Pressure-Based Microfluidic Flow Control for Organ on Chip applications

The importance of flow stability in microfluidic systems is demonstrated by performing cell culture using peristaltic pumps or pressure-based flow controllers.

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Microfluidic Application Notes
Cancer Cell Analysis Made Easy with Aria: cell Capture and Labeling

We have demonstrated the use of Aria and its related software for the automated delivery of different liquids for the capture and labeling of cancer cells (breast cancer) using a complex microfluidic setup.

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