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Double Emulsion Production Station

The Fluigent double emulsion production station is a robust and complete system for producing outstanding monodispersed double emulsion in one single device.

Its performance is coming from the combination of Fluigent’s LineUP microfluidic pumps and the RayDrop device, a breakthrough technology for high monodispersed and stable double emulsion. 

Quick look at the station

Videos

Tutorial on how to produce double emulsion

[REPLAY WORKSHOP] Master the double emulsions production

BENEFITS AND FEATURES

High monodispersity: 2%

Double emulsion size: 80µm-150µm

High frequency: >kHz

Adjustable shell thickness

Control website

No surface coating

w/o/w and o/w/o double emulsion

Comparison with another production method

Batch method Fluigent microfluidic method
ProcessMultiple step processDirect double emulsion production
HomogeneityRandom distribution of single and double emulsionHigh homogeneity
Particle size distributionMore than 50 %~2 %
ReproducibilityLowHigh
Live particle size controlNoPrecise
Continuous (/in line) productionNoYes

Components of the pack

Double emulsion station setup

3 x LineUp Flow EZ (2000 mbar)

Pressure controller

Flow EZ pressure controller Lineup

1 x LineUp LINK

Connection to the PC
(LU-LNK-0002)

Link module communication hub lineup Fluigent

3 x FLOW UNIT
(2 M and 1 L)

Flow meter

3 x P-CAP
(2 x 50mL
+ 1 x 15mL)

Sample reservoirs

Fluigent P-CAP air-tight metal cap pressurized reservoir with falcon tube

1 x SwitchEZ

Communication hub

microfluidic switch ez

3 x 2-Switch

Microfluidic valve

1 x RayDrop

Droplet generator

RayDrop microfluidic droplet generator

Reagents

dSurf

ICO_ACC_007 Icon dsurf

Digital high-speed microscope

(optional)

News-microscope

Specifications

Chip characteristics
DesignCo-flow focusing design
Inputs & Outputs3 inputs, 1 output
Types of emulsionWater-in oil-in-water and oil-in-water-in-oil double emulsion
Double emulsion size*Shell: from 70 to 130 µm, Core: from 20 to 120 µm
MonodispersityCV <2%
Generation rate5 000 Hz (measured for the smallest double emulsion size) can go higher under specific conditions
Capillaries dimensionNozzle: Core: 30 µm & ID Shell: 70 µm ID
Output: 150 µm ID
Wetted material continuous phasePEEK, FEP, glass, stainless steel 316L, poluimide, Viton (seal), resin (nozzle)
Wetted material dispersed phasePEEK, FEP, Glass, resin (nozzle)
Fluids compatibilityWater, mineral oil, fluorinated oil, ethanol, ethyl acetate, IPA, Acetone, Chloroform
Flow control
Pumps**Fluigent Flow EZ (2000mbar)
Flow sensors**Fluigent FLOW UNIT (M & L)
Automated valves**Fluigent 2-SWITCH
Imaging (optional)
MicroscopeFluigent Digital high-speed microscope
Software
Live controlFluigent A-i-O
Automated controlFluigent MAT
ImagingPixelink Capture Software

*Depends on fluids physical properties

**For detailed specification: download LineUP User Manuel, ESS User Manuel

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