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ORGAN-ON-A-CHIP

The science of flow control for Organ-on-a-chip applications

Beonchip X Fluigent Organ On Chip Platform
Beonchip X Fluigent Organ On Chip Platform
Beonchip X Fluigent Organ On Chip Platform 2

Organ on chip study is an emerging field that brings substantial benefits compared to conventional cell culture. In many labs, considerable effort is put in choosing the right chip design, but the impact of flow control is still undetermined. It is our intent to create awareness on the importance of flow and its effects on ones studies.

Read our expertise page to know more about the benefits of flow control in cell culture, learn more about our products and those of our partners (Beonchip) as well as reach out to our team of expert to find a solution that best meets your needs.

Watch our latest webinar replay and move towards to next generation of cell culture.

WHY IS FLOW CONTROL IMPORTANT?

Implementing perfusion and automated fluid delivery in cell biology protocols offer major advantages. Automation increases reproducibility compared to manual pipetting, saves times and improves the level of control of the experiment as all the parameters are tightly regulated (time of delivery, volume and speed of injection). As a result, experimental productivity is enhanced, experiments are transferable between labs and results are more reliableread more

Flow control technologies available on the market

Multiple flow control technologies are available for sub-millimeter range fluid management. Syringe and peristaltic pumps were the first instruments to be used as they were the two available solutions on the market. As demand for microfluidic pumps with higher flow stability, fast response time, versatility and automation capabilities has increased, pressure controllers have become the device of choice. Fluigent offers a wide range of solutions in this area… read more

Benefits of flow by application

fig7

BENEFITS OF MICROFLUIDIC TO BUILD RELEVANT IN VITRO MODELS

The microfluidic chip fabrication process permits one to understand and control interactions between cells, substrates, and the surrounds medium, physically as well as biochemically….

microfluidic cell culture

CONTROLLED SHEAR STRESS

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compressive stress

HOW TO REPRODUCE ACTIVE BIOMIMETIC STIMULATION IN VITRO?

It is proven that mechanical stimulation of intervertebral disc cells (that mostly originates from compression) acts as an important….

microfluidic cell culture

GENERAL INTRODUCTION TO BIOMECHANICS

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microfluidic cell culture

FLOW INDUCED LAMINAR AND PULSATILE SHEAR STRESS

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compressive stress

ACTIVE BIOMIMETIC
SIMULATION

It is proven that mechanical stimulation of intervertebral disc cells (that mostly originates from compression) acts as an important….

stable-biochemical-gradient-fluigent

WHY IS BIOCHEMICAL ENVIRONMENT IMPORTANT?

Cells are constantly exposed to biochemical stimulation from the early embryonic stage to adult life. The spatiotemporal regulation of these signals is essential as it determines cell fate….

PACKS AND APPLICATIONS

The Flow EZ is optimized to monitor or control pressure and flow rate. Constant flow as well as  flow rate pulses, ramps, or sine waves can be generated by the system. The compact system may be controlled manually or remotely and is compatible with cell culture experiments.

PERFUSION PACKS

Discover more about organ-on-a-chip perfusion pack.

 STARTER PACK
 PN: OOC-ST-001

This pack is the perfect set for cell perfusion. It combines a pressure controler and a flowmeter to control either pressure or flow rate. It adapts to any chip. The ability to control flow either manually or automatically using the software makes it the perfect choice for cell biology experiments. This package has been designed to make microfluidic easy even for beginners.

Categorie Product Unit
Microfluidic pump Fluigent's LineUp Flow EZ 1
Flow sensor Fluigent's Flow Unit 1
Chips BEOnChip's chip 1
Connectors Fluigent's kit 1
Tubing Fluigent's kit 1
Software Fluigent's AiO and MAT 1
beonchip, organ on chip

 HIGH THROUGHPUT PACK
 PN: OOC-HT-001

This pack has been designed for high throughput experiments. The compactness and arrangement of the different elements have been optimized to perfuse a maximum number of chips in an incubator. It includes a 8 channel compact pressure controler, a flow rate platform and reservoir holders directly adapted on an incubator trail.

Categorie Product Unit
High throughput microfluidic pump Fluigent’s MFCS-EX (up to 8 channels) 1
Flow rate platform Fluigent's Flow Unit (up to 8 flow unit packed in one platform) 1
Incubator grid On demand custom grid with reservoir holders 1
Chips BEOnChip's chip 1
Connectors Fluigent's kit 1
Software Fluigent's MAT 1
Organ on a chip high troughput pack

PERFUSION APPLICATION NOTE

COC-img1

CARTILAGE ON CHIP

In this application note, we report on the use of Fluigent products to create complex mechanical stimulation patterns on 3D cell culture in a microfluidic platform, or so-called organ-on-a-chip device….

Microbeads

ORGANOIDS CULTURE IN MICROBEADS


Microbead-based microfluidics
has been successfully adapted to cell culture to encapsulate cells in micron size hydrogel beads with high reproducibility and high throughput screening capacities

evaluate cell growth

PRESSURE MEASUREMENT TO EVALUATE CELL GROWTH

This application presents a simple method to monitor cell proliferation in microfluidic chips in real-time. This is demonstrated experimentally using a custom microfluidic chip…

Myochip Microfluidic Chip

CELL SEEDING IN HYDROGELS

Myochip aims to reproduce both the extracellular matrix and the spatial positioning of the 3 different cell types that constitute muscle: muscle cells, endothelial cells and neurons….

PERISTALTIC PUMP VS PRESSURE PUMP

WHY CHOOSE A PRESSURE BASED PUMP OVER A PERISTALTIC PUMP FOR CELL CULTURE?

To demonstrate the importance of flow stability in vascular models, endothelial cells seeded in microfluidic chips were perfused either using a peristaltic pump or pressure-based flow controllers….

Unidirectional recirculation is needed for long term perfusion. For most cells, constant flow is  essential as pulsatile flows can induce mechanical stress and strongly affect cell adhesion and cell survival.

Our recirculation kit combines our Flow-Ez controller with our  L-Switch recirculation valve to provide you the best of our technology.

Read our application note to better capture the importance of the constant flow to grow cells on chips.

Reach out to us to learn about our recirculation kit.

package BE transFLOW

 RECIRCULATION PACK
 PN: OOC-RC-001

Categorie Product Unit
Microfluidic pump Fluigent's LineUp Flow EZ 2
Flow sensor Fluigent's Flow Unit 1
Recirculation valve Fluigent’s L-Switch 1
Chips BEOnChip's chip 1
Connectors Fluigent's kit 1
Tubing Fluigent's kit 1
Software Fluigent's AiO and MAT 1

RECIRCULATION APPLICATION NOTE

PERISTALTIC PUMP VS PRESSURE PUMP

WHY CHOOSE A PRESSURE BASED PUMP OVER A PERISTALTIC PUMP FOR CELL CULTURE?

To demonstrate the importance of flow stability in vascular models, endothelial cells seeded in microfluidic chips were perfused either using a peristaltic pump or pressure-based flow controllers….

In most biological protocols, cell mediums or reagents must be changed during the course of the experiment. To minimize the chance of contamination related to manual operations, gain productivity, reproducibility, and control of your experiments, automation is key.

Aria is the best solution to automate your lab work.  Highly flexible, it can adapt to any perfusion chamber and any protocol thanks to its intuitive software.

Aria is also a tool to automate complex protocols for live-cell imaging (immunostaining, omics applications, radio-metric imaging).

Read our application note to appreciate how a  complex protocol to capture and stain circulating tumor cells in a chip was easily performed using Aria.

Automated sequential injection

 Multiple fluid delivery protocols pack
 PN: OOC- MD-001

Categorie Product Unit
Multiple fluid delivery instrument Aria 1
Software Aria's software 1
Chips Beonchip’s chips 1

MULTIPLEXED FLUID DELIVERY APPLICATIONS

tumor-cell-circulating

TUMOR CELL IMMUNOSTAINING IN CHIP

This application note describes the use of the new Fluigent Aria – a software-assisted instrument capable of delivering of up to 10 different solutions

select the chip for your application

BE-Flow is Beonchip’s most easy-to-use device dedicated to cell culture under flow. It allows the performance of long term 2D or 3D culture in two independent channels. BE-Flow is an optimal device for vascular research where shear stress plays a major role in gene expression.

organ on chip

BE-Doubleflow is Beonchip’s most advanced device. It consists of two perfusable channels connected via a porous membrane. Explore the crosstalk between different 2D and 3D cultures in a biomimetic environment and control the efficiency of the interaction by selecting the optimal pore size for your application. This is the optimal device to study chemotaxis, biochemical gradient and to air-liquid interfaces.

organ on chip

BE-Transflow is Beonchip’s most versatile cell culture platform. It allows the study of complex culture configurations by joining a culture well with a microfluidic channel via a porous membrane. This is the optimal device for Air Liquid Interface (ALI) culture, endothelium/epithelium barrier, crosstalk studies and to mix 2D and 3D cell culture.

organ on chip

BE-Gradient: is Beonchip’s device designed for the application of electrochemical gradients to 3D cell cultures. BE-Gradient is compatible with any type of optical microscopy (inverted phase contrast, confocal, fluorescence..). Be-Gradient consists of a central chamber for cell culture in 3D in contact with two lateral channels. Lateral channels are meant to simulate blood vessels. 2D culture is also possible for adherent cells not only in the central chamber but also in the lateral channels

organ on chip

MORE ABOUT ORGAN-ON-A-CHIP

WHAT IS ORGAN ON A CHIP?

NEW TO ORGAN-ON-A-CHIP?
In 2010 that the term organ-on-a-chip (OOAC) was invented by Ingber, et. al., who developed a microfluidic chip to capture organ-level functions of the human lung. Microfluidics enables one the unique ability to control the cellular microenvironment with high spatiotemporal precision… read more

Watch our latest webinar replay and move towards to next generation of cell culture.

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