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LineUp™ Push-Pull

LineUp Push-Pull Fluigent pressure and flow controller for positive pressure and vacuum in microfluidics
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The LineUpTM Push-Pull is a stand alone controller with the ability to deliver finely regulated pressure or a vacuum through a single outlet over the range of -800 to +1000 mbar. Suited for any application within this range, it brings the existing benefits of the LineUpTM series with expanded capabilities as well as superior stability and high performance.

Benefits

icon ease of use

Ease of use

Icon no PC requirement needed

Control with or without a PC

Pressure & Vacuum Control in one unit

Icon modular and expandable

Modular & Expandable

Icon compactness

Compact

Applications

Microfluidics
For experiments where combined or alternating pressure and vacuum based fluid movement, aspiration and dispensing can be accurately controlled

Flow Recirculation
The combination of vacuum and pressure can be used to redirect the flow bidirectionally

Deliver small volumes with no waste
When samples from 5µL to 500 µL need to be injected to a chip, the tubing may be used as a reservoir. The main reservoir may be filled with another liquid, or air may be used to drive the liquid introduction

Specifications

*Maximum range depends on the vacuum pump performance
**Response time includes settling time and pressurization time

Software Compatibility

Icon of MAT Fluigent software for automation protocole in microfluidic experiments

The LineUP series modules are compatible with Fluigent’s newest software applications:

LineUp LINK

Connection to the PC
(LU-LNK-0002)

Link module communication hub lineup Fluigent

FLOW UNIT

Flow meter

LineUp Adapt

Pressure reducer
(P/N: LU-ADP-0001)

Adapt pressure reducer Lineup

P-CAP

Pressurized reservoirs

Fluigent P-CAP air-tight metal cap pressurized reservoir with falcon tube

LineUp Flow EZ

Pressure controller

Flow EZ pressure controller Lineup

LineUp supply kit

Pressure & power supply

Fluigent LineUp power and pressure supply kit

A-i-O

Monitoring software

Icon of All in one software by Fluigent

MAT

Automation software

Icon for software Microfluidic automation tool by Fluigent

FAQ's

Yes, the LineUp Push-Pull can be used as a regular Flow EZ up to 1000 mbar or a negative Flow EZ down to -900 mbar. Connect the power supply and the required pressure or vacuum source to get the device ready to use. In order to use it with just a vacuum supplied you will have to bypass the pressure check window (see “How to bypass the pressure check window”).

 

If the input pressure  is too low or too high, the pressure check window will display the current pressure supply value and the target pressure value to operate. Adjust the pressure source to match the target pressure and the operational window will automatically appear.

 

The pressure check window indicates if the pressure supplied meets the input requirement of the device., One cannot access to the operational window until they provide the required pressure supply value or intentionally bypass the check window.

In order to bypass the pressure check window and directly access to the operational window, one must :

  1. Ensure the LineUp Push-Pull is turned OFF or disconnected from power supply (If a LINK module is connected press and hold the button for several seconds to turn OFF the power)
  2. Reconnect the power supply to the device (or turn the LINK module ON by pressing its button again)
  3. While the device is turning ON, press and hold either the LineUp Push-Pull « P=0 » button (top right) or bottom left button for several seconds in order to bypass the check window.

Please note that if the input pressure goes above the maximum pressure of the device, the device will go into a security mode and lock itself out. This is done to prevent internal damage to the sensors. To unlock, reduce the input pressure and then press the button under the “unlock”  text on the screen (typically bottom left button)

Lexan LineUp Push-Pull to show the different buttons and how to by pass the pressure check window

While using a vacuum or when the reservoir is full, there is a risk of backflow into the pneumatic tubing which could end up going into the device.

One solution would be to insert a backflow filter between the reservoir and the pneumatic tubing however this could affect the device performance by slowing the pressure response time.

When using a vacuum,  a solution is to use a fluid reservoir such as a PCAP to trap any liquid between the device’s fluid outlet and the Flow-EZ vacuum channel

Scheme with LineUp Push-Pull use of an overflow flask to avoid backflow into the device

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