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LineUp™ Push-Pull

LineUp Push-Pull Fluigent pressure and flow controller for positive pressure and vacuum in microfluidics
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The LineUpTM Push-Pull is a stand alone controller with the ability to deliver finely regulated pressure or a vacuum through a single outlet over the range of -800 to +1000 mbar. Suited for any application within this range, it brings the existing benefits of the LineUpTM series with expanded capabilities as well as superior stability and high performance.


icon ease of use

Ease of use

Icon no PC requirement needed

Control with or without a PC

Pressure & Vacuum Control in one unit

Icon modular and expandable

Modular & Expandable

Icon compactness



“The Fluigent LineUp series, including the new push-pull pump, enables precise and highly controlled aspiration and respiration of liquids. The set-up allows us to further advance our research in both continuous flow and droplet microfluidics.”

Prof. Jeroen Lammertyn, KU Leuven Belgium – Biosensors group.

The device is well designed and allows for easy control of my microfluidic chips. What I like most is that you are independent of a computer and can directly control both positive and negative pressure.” 

Christoph Trenzinger  – Stratec


For experiments where combined or alternating pressure and vacuum based fluid movement, aspiration and dispensing can be accurately controlled

Flow Recirculation
The combination of vacuum and pressure can be used to redirect the flow bidirectionally

Deliver small volumes with no waste
When samples from 5µL to 500 µL need to be injected to a chip, the tubing may be used as a reservoir. The main reservoir may be filled with another liquid, or air may be used to drive the liquid introduction


*Maximum range depends on the vacuum pump performance
**Response time includes settling time and pressurization time

Software Compatibility

oxygen software

The LineUP series modules are compatible with Fluigent’s newest software applications:

  • The Fluigent Oxygen software: the new way to get full control of your microfluidic setup. It combines in one program all the functions and capabilities of our traditional software: A-i-O, MAT, ESS control and much more. Through its intuitive dashboard, OxyGEN is our new reference tool for real-time control and for developing time based protocols focusing on pressures, flow rates, volumes, and valve control in microfluidic experiments.
  • Software Development Kit: Integrate the LineUp Flow EZ™ seamlessly in your existing application. Available in C++, Labview, Matlab, Python, etc. More information on the SDK »


Connection to the PC

Link module communication hub lineup Fluigent


Flow meter

LineUp Adapt

Pressure reducer
(P/N: LU-ADP-0001)

Adapt pressure reducer Lineup


Pressurized reservoirs

Fluigent P-CAP air-tight metal cap pressurized reservoir with falcon tube

LineUp Flow EZ

Pressure controller

Flow EZ pressure controller Lineup

LineUp supply kit

Pressure & power supply

Fluigent LineUp power and pressure supply kit

Oxygen software

Real-time control & automation software

oxygen software


Yes, the LineUp Push-Pull can be used as a regular Flow EZ up to 1000 mbar or a negative Flow EZ down to -900 mbar. Connect the power supply and the required pressure or vacuum source to get the device ready to use. In order to use it with just a vacuum supplied you will have to bypass the pressure check window (see “How to bypass the pressure check window”).


If the input pressure  is too low or too high, the pressure check window will display the current pressure supply value and the target pressure value to operate. Adjust the pressure source to match the target pressure and the operational window will automatically appear.


The pressure check window indicates if the pressure supplied meets the input requirement of the device., One cannot access to the operational window until they provide the required pressure supply value or intentionally bypass the check window.

In order to bypass the pressure check window and directly access to the operational window, one must :

  1. Ensure the LineUp Push-Pull is turned OFF or disconnected from power supply (If a LINK module is connected press and hold the button for several seconds to turn OFF the power)
  2. Reconnect the power supply to the device (or turn the LINK module ON by pressing its button again)
  3. While the device is turning ON, press and hold either the LineUp Push-Pull « P=0 » button (top right) or bottom left button for several seconds in order to bypass the check window.

Please note that if the input pressure goes above the maximum pressure of the device, the device will go into a security mode and lock itself out. This is done to prevent internal damage to the sensors. To unlock, reduce the input pressure and then press the button under the “unlock”  text on the screen (typically bottom left button)

Lexan LineUp Push-Pull to show the different buttons and how to by pass the pressure check window

While using a vacuum or when the reservoir is full, there is a risk of backflow into the pneumatic tubing which could end up going into the device.

One solution would be to insert a backflow filter between the reservoir and the pneumatic tubing however this could affect the device performance by slowing the pressure response time.

When using a vacuum,  a solution is to use a fluid reservoir such as a PCAP to trap any liquid between the device’s fluid outlet and the Flow-EZ vacuum channel

Scheme with LineUp Push-Pull use of an overflow flask to avoid backflow into the device

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