High Throughput Cell Perfusion Pack

[OOC-ST-001]

The most efficient system for creating high throughput cell perfusion

Our cell perfusion pack is designed for high throughput experiments and optimized to perfuse chips while growing multiple organ models in an incubator. It includes an 8-channel compact pressure controller, a flow rate platform, and reservoir holders directly adapted on an incubator trail.

This setup features an intuitive interface that mimics physiological conditions to perform long-term experiments without additional effort while also ensuring high reproducibility and scalability.

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Main benefits

Designed for cell biology
Contamination-free, sterile
Easy to use
Simple connections
Multiplexing
Designed for experiments parallelization

What’s included in the pack

Fluigent’s MFCS-EX: A microfluidic flow controller with 8 customizable channels with different pressure ranges for high precision operations in microfluidic experiments.
Fluigent’s Flow Unit : Bidirectional microfluidic flow sensors available in multiple low-rate ranges – up to 8 flow units can be used in one platform.
Incubator grid: An on-demand, customizable grid compatible with the incubator along with reservoir holders.
Fluigent connectors and tubing kit.

Features of the cell perfusion system

Stable & Complex Flow Patterns

Fluigent products have unprecedented performances in terms of precise control, stability,and responsiveness. High stability is particularly useful for applications with constant shear stress, like vascular perfusion.

Our Cell Perfusion Pack creates high responsiveness in the reproduction of complex flow patterns such as aortic pressure variations. This level of control ensures consistent and reproducible experimental conditions, minimizing experimental variability.

Protocol automation & user-friendly interface

Once the parameters are set and optimized, protocol automation is the key to saving time, limiting contamination, and decreasing variability. Using any protocol, valves, or pressure, Fluigent flow controllers can be assembled to automate protocols using user-friendly software (OxyGEN).

OxyGEN
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Researchers can quickly become proficient in using the system, reducing the learning curve, and maximizing productivity.

Versatility

This package can be used for diverse applications with any microfluidic chip.

Enhanced High-Throughput Capabilities

With the ability to run multiple assays in parallel, our perfusion pack is specifically designed to accommodate high-throughput experimentation while efficiently generating data. Compatible with Various Applications

The package is versatile and suitable for a wide range of applications, including drug screening, disease modeling, toxicity testing, and personalized medicine studies.

Customization

Our high throughput perfusion pack is flexible and adaptable to various organ-on-a-chip models and research needs (specific flow rates, pressure, etc.) The cell perfusion package’s modularity allows researchers to design experiments tailored to their unique research questions.

Related applications

Microfluidic perfusion for cell culture

Microfluidictechnology has revolutionized cell culture systems by enabling precise control over fluid flow and creating microenvironments that mimic physiological conditions. One key technique in microfluidics is cell perfusion, which involves the controlled flow of fluid over cells or tissues to maintain their viability and functionality.

Cell perfusion begins with the design of a microfluidic device that incorporates intricate channels or networks, typically on a micrometer scale. Cells are then seeded onto the device, either as a monolayer or in 3D structures, and a continuous flow of culture media or other desired fluids is established within the device. This flow is carefully regulated by a pressure-based controller to provide nutrients, remove waste products, and maintain a stable microenvironment.

The ability to precisely control the flow rate and duration of perfusion allows researchers to mimic physiological conditions, such as blood flow rates in blood vessels. Real-time monitoring and analysis of parameters like cell viability, proliferation, and metabolic activity provide valuable insights into cellular behavior within these microenvironments.

What are possible applications for our cell perfusion pack?

Drug Screening and Development

This setup facilitates the testing of potential drug candidates under more realistic physiological conditions. High-throughput capabilities enable the screening of numerous drug compounds simultaneously, accelerating the drug discovery process and reducing costs.

In this example of the application, Chakrabarty et al. (1) developed a novel microfluidic Cancer-on-a-Chip platform to evaluate patient treatment responses using controlled growth conditions for tumor tissue slices. This system allows for the effective prediction of treatment responses for breast and prostate tumor models, and the culture period could be extended up to 14 days without significant changes in tissue quality.

Figure 1: Cross-section of Cancer-on-a-Chip illustrating diffusion and perfusion toward the tissue slice. The CoC platform is connected to Fluigent’s High throughput cell perfusion pack for the entire culture period (1).

Disease Modeling and Pathophysiology Studies

By investigating cellular responses to disease-related factors, this cell perfusion pack could provide valuable insights into disease pathophysiology, facilitating the development of targeted therapies.

In this example, Messelmani et al. (2) developed a new liver-on-a-chip model integrating a hydroscaffold, allowing cells to organize into complex 3D spheroid architecture. This model could play a role in producing a promising device for disease modeling, drug screening,and risk assessment.

Figure 2: Characterization of liver spheroids after 21 days of dynamic culture in a biochip containing a 3D hydroscaffold, using Fluigent’s MFCS (2). F-actin and MRP2 staining show the formation of bile canalicular-like structures, mimicking in-vivo liver behavior: DAPI (nuclei, blue), phalloidin (F-actin, green); MRP2 (red) and a biliary-like network

Another example of disease modeling using Fluigent’s MFCS pack is the work of Paloschi et al. (3). They developed an Artery-on-a-Chip model mimicking the arterial vessel wall by incorporating structural aspects of the vasculature (lumen-intima-media) as well as hemodynamic forces impacting the luminal cells. They were able to characterize novel targets previously unrelated to vascular diseases, and to demonstrate that this model system could be used as a platform for testing novel therapeutic agents.

Figure 3: Scheme represents the utilization of the Artery-on-a-Chip, developed by Paloschi et al. (3) as a translational tool for disease modeling and drug testing.

Toxicity Testing and Safety Assessment

High-throughput capabilities of our high throughput cell perfusion pack enable researchers to screen multiple compounds for potential toxicity, helping identify safe and effective substances for further development.

Tissue Engineering and Regenerative Medicine

Researchers can employ the cell perfusion pack to create dynamic microenvironments that promote tissue growth and regeneration.

The platform enables the study of tissue development, cell behavior, and the interactions between different cell types, aiding tissue engineering efforts.

Understanding how tissues respond to different growth factors and conditions can enhance regenerative medicine approaches and tissue transplantation strategies.

Specifications

Fluigent package

Fluigent’s MFCS-EX (up to 8 channels)

Fluigent’s Flow Unit (up to 8 flow unit packed in one platform)

Incubator grid (On demand custom grid with reservoir holders)

Beonchip microfluidic chip

Fluigent connectors and tubing kit

OxyGEN

Control in real-time, protocol automation, data record and export

ver. 2.2.0.0 or more recent

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Software Development Kit

Custom software application

ver. 22.2.0.0 or more recent

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Expertise & resources

Accessories

References:

Chakrabarty S, Quiros-Solano WF, Kuijten MMP, Haspels B, Mallya S, Lo CSY, Othman A, Silvestri C, van de Stolpe A, Gaio N, Odijk H, van de Ven M, de Ridder CMA, van Weerden WM, Jonkers J, Dekker R, Taneja N, Kanaar R, van Gent DC. A Microfluidic Cancer-on-Chip Platform Predicts Drug Response Using Organotypic Tumor Slice Culture. Cancer Res. 2022 Feb 1;82(3):510-520. doi: 10.1158/0008-5472.CAN-21-0799. Epub 2021 Dec 6. PMID: 34872965; PMCID: PMC9397621.

Messelmani T, Le Goff A, Souguir Z, Maes V, Roudaut M, Vandenhaute E, Maubon N, Legallais C, Leclerc E, Jellali R. Development of Liver-on-Chip Integrating a Hydroscaffold Mimicking the Liver’s Extracellular Matrix. Bioengineering (Basel). 2022 Sep 5;9(9):443. doi: 10.3390/bioengineering9090443. PMID: 36134989; PMCID: PMC9495334.

Utilization of an Artery-on-a-chip to unravel novel regulators 2 and therapeutic targets in vascular diseases 3 4 Valentina Paloschi1,2*, Jessica Pauli1,2, Greg Winski3 , Zhiyuan Wu1,4, Zhaolong Li1 5 , Nadiya Glukha1 , Nora Hummel1 , Felix Rogowitz5 , Sandro Meucci6 , Lorenzo Botti7 , Albert Busch1,8 6 , Ekaterina Chernogubova4 , Hong Jin4 , Nadja Sachs1 , Hans-Henning Eckstein1 7 , Reinier A. Boon9,10,11, Andreas R. Bausch12, Lars Maegdefessel1,2,3 https://www.biorxiv.org/content/10.1101/2022.11.29.517312v1

High Throughput Cell Perfusion Pack

What’s included in the pack

MFCS™ series

FLOW UNIT | FLOW UNIT +