Drop-Seq package

[ODROPSEQFPCK]

    Complete system for starting Drop-Seq experiments

    For optimized Drop-Seq experiments in Next-Generation Sequencing (NGS). The Fluigent Drop-Seq package allows better reproducibility and control of both single cells and beads encapsulation.

    Main benefits
    • Biocompatible
      Efficient

      High encapsulation efficiency

    • Gain time

      Faster than standard Drop-Seq protocol

    • droplet monodispersity
      Reproducible

      High monodispersity (2%)

    Features

    Latest design

    Each droplet generation device is based on the design recommended in the latest McCarroll lab Drop-Seq protocol, ensuring the best chances of success.

    Fluigent devices

    Precision engineered robust devices durable over a wide range of pressures, temperatures, and flow rates Last

    23 designs per chip

    Provides value for money in a  chip that lasts.  When the life of one device is depleted, simply move onto the next one

    Produce monodisperse droplets

    Reliable and consistent generation of droplets of optimal size for Drop-sequencing

    Efficient  production  of  transcript  libraries

    Superior  design  promotes  optimal  mixing  of  component fluids, thereby minimizing bead shearing or premature lysis of cells and mRNA release

    Why use pressure for Drop-Seq?

    While the original protocol was developed using syringe pumps, pressure-controlled systems have a few advantages.

    First, in terms of raw performance, pressure-driven systems are faster to set up, easier to control, and more stable over time, which allows for an emulsion of better quality: the droplet size will be more homogeneous, and start/stop populations will be smaller. This leads to better segregation of the cells, and less reagent use and less sample loss.

    As the sample and beads need to be agitated throughout the experiment, it is usually done with a bulky stirring bar for syringe pumps. This leads to a dead volume that will not be injected into the chip. Pressure-driven setups can be run using more conventional containers that make it possible to use an external agitation system, such as a standard lab vortexing system.

    Using Fluigent setup for Drop-Seq experiment with pressure controller allow then to:

    • Gain time: (less than 1 minute to obtain droplet compare to macosco few minute)
    • Avoid losing reagents (cells or beads) during transition time
    • Have better control and avoid the problems that could appear with syringe pump. Such as backflow of the beads inside the cells channel which could modify drastically the experiment.

    References

    [1] Théry M, Bornens M, Get round and stiff. 2008, HFSP J, 2(2):65-71.

    [2] Tavares S et al, actin stress fiber organization promotes cell stiffening and proliferation of pre-invasive breast cancer cells. 2017, Nat Commun. 8:15237.

    [3] Guo Q et al, Microfluidic biomechanical assay for red blood cells parasitized by Plasmodium falciparum. 2012, Lab Chip; 12(6):1143-50.

    [4] Yanez LZ et al, human oocyte developmental potential is predicted by mechanical properties within hours after fertilization, 2016, Nat Commun. 7:10809

    Main products of the package

    Specifications

    LineUp Flow EZ pressure controller (2 bar) x3
    FLOW UNIT M x2
    FLOW UNIT L x1
    LineUp LINK Module (software control) x1
    P-CAP 15 mL x1
    P-CAP 2 mL x2
    Drop-Seq chip
    LineUp Supply kit x1
    Tubing & fitting kit x1

    OxyGEN

    Control in real-time, protocol automation, data record and export
    ver. 1.0.0.0 or more recent

    See the offer


    Software Development Kit

    Custom software application
    ver. 21.0.0.0 or more recent

    See the offer