Drop-Seq Package
Quick look at Fluigent's Drop-Seq solution
CONTENTS AND SETUP
FLOW-EZ™ (x3)
Pressure based flow controller.
LINK
Control your system with Fluigent software.
FLOW UNIT M (x2)
High precision flow sensor for the dispersed phase.
FLOW UNIT L
High precision flow sensor for the continuous phase.
P-CAP 15 mL
Air-tight pressure cap for 15 mL reservoirs.
P-CAP 2 mL (2x)
Air-tight pressure cap for 2 mL reservoirs.
Drop-Seq Chip
FlowJEM PDMS Microfluidic Devices
A-i-O Software
Software to monitor and control in real time.
Flow EZ supply kit
Kit to provide pressure and power to the Flow EZTM.
Standard connector and tubing kit
Microfluidic and pneumatic tubing & fitting kit for standard setup.
Optional products
P/N: IMCA001 | DR-RE-SU-12 | FLPG+
12 mL dSurf
High-performance surfactant dedicated to microdroplet generation
Digital High-Speed Microscope
Observe and record your results with high resolution and frame rate.
(Resolution : 2592 x 2048, Frame rate : 7092 fps)
FLPG+
High quality air for microfluidic
External products
Barcoded beads
Contact Chemgenes for information (product number: CSO-2011)
Vortex mixer
Available from several suppliers
Why using pressure for Drop-Seq?
While the original protocol was developed using syringe pumps, pressure-controlled systems have a few advantages.
First, in terms of raw performance, pressure driven systems are faster to set up, easier to control and more stable over time, which allows for an emulsion of a better quality: the droplet size will be more homogeneous and start/stop populations will be smaller. This leads to better segregation of the cells, and less reagent use and less sample loss.
As the sample and beads need to be agitated throughout the experiment, it is usually done with a bulky stirring bar for syringe pumps. This leads to a dead volume that will not be injected in the chip. Pressure driven setups can be run using more conventional containers that make it possible to use an external agitation system, such as a standard lab vortexing system.
Using Fluigent setup for Drop-Seq experiment with pressure controller allow then to:
- Gain time: (less than 1 minute to obtain droplet compare to macosco few minute)
- Avoid losing reagents (cells or beads) during transition time
- Have better control and avoid the problems that could appear with syringe pump. Such as backflow of the beads inside the cells channel which could modify drastically the experiment.
