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Webinars

Reserve your space for one of our free Webinars to learn about basic and advanced microfluidic topics, get tips and techniques for your lab, or find out more about new technologies and products. The Webinars will cover a range of topics including particle/droplet formation, microfluidic basics, applications in cell biology, and more.

The live presentations will include the opportunity to question our specialists via chat. If you can’t attend a presentation – past Webinars are available for on-demand viewing.

Upcoming Webinars

LIVE WORKSHOP

MASTERING THE PRODUCTION OF DOUBLE EMULSIONS

Free registration / limited to 15 participants per session

OVERVIEW:

Are you working in academia or industry in the field of cosmetics, food, pharmaceuticals, or chemical processes? If you are looking for solutions and methods to improve and gain performance in generating the right double-emulsion for your needs, this online workshop may be of benefit to you.

Until now, generating double emulsions has been a  challenge as existing technologies  suffer from various limitations (low reproducibility, need for multiple surface coatings, poor size distribution, etc)

During this workshop, you will:

  • Learn how to generate W/O/W double emulsions
  • Have live discussions with our experts regarding your specific application
  • Gain insight on the newest material and method for the generation of highly monodispersed double emulsions

SPEAKERS:

Adam Meziane, Particle generation product Manager, Fluigent
Adrien Dewandre, Technology Lead Encapsulation, Secoya Technologies
Youen Vitry, Technology Lead Encapsulation, Secoya Technologies

AGENDA:

  1. Bref introduction to the Double Emulsion Formation (5 mins)
  2. Live Demonstration in the laboratory to learn how to make double emulsions (30 mins)
  3. Learn how to use double emulsions to produce microcapsules (Resin, PLGA, Alginate…) (5 mins)
  4. Interactive Q&A session to discuss your application needs (20 mins)

DATES & TIMES:

1 workshop, 4 sessions

  • Session 1: Tuesday 27th April      10:00-11:00 AM CET  (Full)
  • Session 2: Tuesday 27th April        4:00-5:00 PM CST
  • Session 3: Thursday 29th April   10:00-11:00 AM CET
  • Session 4: Thursday 29th April      4:00-5:00 PM CST 

Registration

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    Need more information about double emulsion production?
    Check out our Double Emulsion Production Station page.

Past, On-Demand Webinars

MICROFLUIDIC ELECTRICAL IMPEDANCE SPECTROSCOPY FOR SINGLE-CELL CHARACTERIZATION AND COUNTING

Electrical impedance spectroscopy (EIS) is a technique that guarantees fast throughput and label-free sensing, so that it can be used to characterize electrical properties, size, or morphology of cells in microfluidic devices. Fluigent and Zurich Instruments drew on their expertise to propose a complete solution for EIS in microfluidics.

In this webinar, we present an EIS system consisting of a lock-in amplifier and microfluidic flow controllers and put it to work on detecting microbeads and water-in-oil droplets.

How to turn your fluorescence microscope into a spatial -omics platform

Current approaches in genomics, transcriptomics, and proteomics yield quantitative abundance analysis of biomolecules on an almost routine basis, with paramount impacts in the life sciences. However, coupling this high content to spatial information in a single cell and tissue context is still a challenge and where our efforts are presently focusing.

In this webinar Alvaro will share my facility experience on how to build spatial omics platforms with hope that their experience can serve others in the road ahead to decide which technique is best for their applications and to assist in their implementation.

Automated protocol to perform microfluidic pumping, mixing and sampling

Microfluidics provides advantages in fluid handling capabilities, scalability, robustness, and automation. In the past ten years, programmable microfluidic platforms have been implemented, allowing to perform typical fluidic operations such as mixing, sampling, washing, and reacting automatically within a single microfluidic chip only by modifying the sequence/order of fluidic operations using a software. They are useful in biological and chemical applications, such as quantitative metabolic biomarker and genetic analysis4,5, protein-based biomarker detection6, or small molecule chemical and environmental analysis7. Among these mechanisms, pneumatic actuation is the most reliable method due to the simplicity of fabrication, ease-of-use, scalability, and a high degree of accuracy, precision, and reliability.

This webinar will show how to combine Fluigent systems and software for the sequential injection of fluids within a microfluidic chip developed by the CEA-LETI to perform pumping, mixing, sampling, and cleaning.

Concepts of microfluidics

This webinar aims to provide a broad introduction to microfluidics by introducing the attendee to microfluidics and related physical principles. The webinar will also feature some typical applications

  • Understand the controlling principles of microfluidics and how to optimize them for your research field (life science, pharmaceutical, analytical chemistry …)
  • You are new to microfluidics and want to expand your overall knowledge of microfluidic systems and related physical principles.

Microfluidic concepts and challenges for the characterization and sorting of bioparticles

The webinar will be divided into two parts. The 1st part will present the research priorities and different microfluidic approaches of the department of Nanobiophotonics of the Leibniz-IPHT in Jena. The second will focus the work of Daniel Kraus, Ph.D. Student at Fluigent/Leibniz-IPHT, on image-based multispectral characterization and sorting of bioparticles: Bioparticles are ubiquity spread entities in nature, medicine and technology. The composition and quality of particles and especially bioparticles gaining increasingly importance in industrial production, material sciences and quality assurance. Morphological properties of particles such as size, diameter as well as spectral information are important quality features for the process control and particle identification. The aim of image-based on-chip spectral classification is to achieve higher accuracy and higher throughput of samples compared to conventional methods.

Scientific applications:

  • microfluidic synthesis of nanoparticles
  • digital PCR
  • droplet generation + sorting
  • image-based flow cytometry
  • microfluidic spectral characterization and sorting of bioparticles

Double emulsion production made easy : a reliable device

Whereas double emulsion is a promising method for many applications, technologies to make double emulsion such as batch methods are all suffering from various limitations (low reproducibility, big size distribution…). During this webinar, we are going to present the only device available on the market which allows to easily produce double emulsion with any kind of solution without chemical surface treatments needed

 

Fast impedance spectroscopy for characterization and counting

Microfluidics benefits greatly from electrical impedance measurements when it comes to measurement speed and sensitivity. In this presentation, we will show how to build a microfluidic electrical impedance spectroscopy platform and include measurement results from an operating microfluidic system. A highly accurate and stable flow rate, critical for most microfluidic applications, is achieved using the LineUp series. The EZ drop can smoothly generate water-in-oil droplets with a wide range of sizes. On the detection part, thanks to the differential current measurement scheme, the HF2LI Lock-in Amplifier can resolve individual flow processes within a 5 µs timeframe, with clearly distinguishable peaks for droplets and microbeads in different sizes. Simultaneous multi-frequency measurements further unveil a full picture of their dielectric properties.

Automate cell studies with Aria

Get the success you deserve with Fluigent’s new perfusion system, Aria that takes care of fluid delivery for you.
If your experiments require precise timed delivery of reagents, probes, fluorophores, etc. while minimizing operator interaction, look no further than our new generation instrument for automating cellular perfusion studies, Aria.
Based on input from our customers, protocols requiring delivering more than one solution demand large amounts of time to set up, as well as significant operator interaction. The Aria provides rapid experimental setup, ease of use, and can automate even multi-day protocols. It can interface with many microscopes to further simplify the process of reagent delivery and imaging.

Drug encapsulation in biocompatible microparticles for drug delivery

In pharmaceutical area, Active Pharmaceutical Ingredient (API) encapsulation into biodegradable and biocompatible polymers is widely used for producing new smart Drug Delivery Systems.  The goal of these drug delivery systems is to supply doses of drugs for a sustained period of time at target specific sites in the body. This can be achieved by efficiently loading drugs into microparticles which will protect the API during physiological transport and release it when the microparticles have reached their specified target location(s).  To regulate the release of the drug, it is critical to controllably produce microparticles with known sizes and a homogeneous size distribution as the rate of drug release is proportional to microparticle size.

Among all techniques available for microparticle production, microfluidic and droplet based microfluidic ones appear as the best solutions to precisely control microparticle production in term of size, API loading and encapsulation efficiency.

In this webinar, we discussed how microfluidics can allow API encapsulation into biocompatible and biodegradable microparticles for drug delivery.

Organ on chip: Towards the next generation of cell culture platforms

Fluigent and Beonchip are partnering to offer a complete solution for organ on chip. This webinar will first introduce Beonchip, their chips and the numerous applications that can be performed. The second part will focus on flow control systems. Although often considered as secondary, flow control is as important as chip design as cells are highly sensitive to mechanical forces. Results demonstrate how cells are affected by peristaltic pumps compared to pressure control systems.

LineUp Series, the new generation of microfluidic controllers

Discover the LineUp™ Series, Fluigent’s leading fluidic delivery platform. A complete solution, including software for automation, to easily adapt to any microfluidic setup and provide unmatched accuracy, simplicity, and versatility.

Raydrop, a universal droplet generator based on a non-embedded co-flow-focusing

Most commercial microfluidic droplet generators rely on the planar flow-focusing configuration implemented in polymer or glass chips. The planar geometry, however, suffers from many limitations and drawbacks, such as the need of specific coatings or the use of dedicated surfactants, depending on the fluids in play. On the contrary, and thanks to their axisymmetric geometry, glass capillary-based droplet generators are a priori not fluid-dependent. Nevertheless, they have never reached the market because their assembly requires art-dependent and not scalable fabrication techniques. Here we present a new device, called Raydrop, based on the alignment of two capillaries immersed in a pressurized chamber containing the continuous phase. The dispersed phase exits one of the capillaries through a 3D-printed nozzle, placed in front of the extraction capillary for collecting the droplets. This non-embedded implementation of an axisymmetric flow-focusing is referred to co-flow-focusing.

Experimental results demonstrate the universality of the device in terms of the variety of fluids that can be emulsified, as well as the range of droplet radii that can be obtained, without neither the need of surfactant nor coating. Additionally, numerical computations of the Navier-Stokes equations based on the quasi-steadiness assumption are shown to correctly predict the droplet radius in the dripping regime and the dripping-jetting transition when varying the geometrical and fluid parameters.In the context of a growing demand of controlled droplets in many areas, the Raydrop emerges therefore as a very robust and versatile solution easily implementable in laboratories with little experience and facilities in microfluidics.

Towards a universal flow sensor for microfluidics

With the development of microfluidics and its applications in life sciences, the need for disposable sensors capable of measuring flow rates over wide ranges and without prior calibration steps has increased. However, commercially available flowmeters or those reported in the literature only partially meet these specifications. The aim of this Ph.D. work has been to address these needs thanks to an innovative measuring principle: a microscopic wheel driven by the liquid motion.

In this webinar we first presented the manufacturing processes implemented, based on the photopolymerization of a photosensitive liquid. As we predicted a linear response of the sensor, independent of the liquid viscosity, and varying according to the wheel radius, we compared the results obtained experimentally to the theoretical model. Then, we discussed the measured wheel movement fluctuations, phenomena that were not initially predicted. Finally, based on a new model that describes the deformation of a microfluidic channel, we discussed the influence of flow asymmetries and the wheel confinement on its rotation.

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