WEBINAR: Single cell encapsulations compatible with FACS sorting, API encapsulations in biocompatible polymers, and more
Until now, performing high efficiency encapsulation processes continuously has been challenging, as existing technologies have limitations (low reproducibility, the need for multiple surface coatings, poor size distribution, etc). The Raydrop encapsulation platform from Secoya solves these issues by providing an easy-to-use system that produces highly monodisperse W/O/W or O/W/O single or double emulsion from 15 µm to 400 µm.
The Raydrop encapsulation platform allows researchers to easily encapsulate single cells inside highly-monodisperse W/O/W double emulsions at a very high rate (>1kHz). Due to their small size (<30 µm), these double emulsions are compatible with FACS sorting. In addition, an injection loop enables users to encapsulate biological compounds with minimum circulation in the tubing, allowing for the injection of low-volume samples and avoiding the contamination of the fluidic line.
The Raydrop encapsulation platform allows researchers to easily perform a wide variety of encapsulation processes. The system’s versatile emulsification capabilities allow for the encapsulation of API in either hydrophilic or hydrophobic polymers (Chitosan, PLGA, Polymethacrylate, etc.). Also, the use of an injection loop enables users to encapsulate low amounts of material (<200 µl) to quickly screen the new formulation.
- Theoretical presentation of the Raydrop’s encapsulation process
- Case study: encapsulation of cells, yeast, and bacteria in a small double emulsion for FACS sorting
- Case study encapsulation of API in microbeads and microcapsules
September 20 – 10 AM CEST – 4 PM CST
September 20 – 5 PM CEST 11 AM EST/8 AM PST
Our Encapsulation Platform for FACS
The cell encapsulation platform includes an organized flow path with pressure controllers, filters, flowmeters, and valves to facilitate the start-up, shut-down, and cleaning of the system between runs. Dedicated optics are included to optimize the visualization of droplet production at high generation frequency.
How to encapsulate bacteria in double emulsions?
In this application note, we associate the double emulsion microfluidic platform with FACS sorting technology to sort out small droplets encapsulating fluorescent e-coli bacteria.