Microfluidic Size Cell Sorting Pack

[ESORT-PCK01]

A complete cell sorting pack for starting size sorting experiments

Why our cell sorting pack? Sorting and separating cells from a heterogeneous cell mixture is a common research application. The expanding need to isolate rare cells increases the demand for cell sorting devices.

Microfluidic cell sorting can be combined with additional fluidic operations for complete lab on-a-chip applications and for diagnostic and therapeutic purposes. These devices use a wide range of techniques to sort cells with specific speeds and efficiencies.

The microfluidic cell sorting pack allows sorting cells or particles according to their size using inertial and Dean drag forces. Fluigent has partnered with microfluidic ChipShop to provide a solution with well-designed microfluidic devices and excellent flow control for sorting experiments.

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Main benefits

Label-free
No labeling steps
High-throughput
Up to 3 mL/min
Passive sorting
No external source required
Flexible
Up to 8 cells/particles sizes sorted per experiment

Features of the cell sorting pack

Complete system

This package includes all the components necessary to start cell sorting experiments.

Engineered solution

We built the package with quality pressure controllers, microfluidic chips, tubing and fittings to provide the most optimized and versatile system.

Customization possible

We can adapt the package to fulfil your needs.

Sorting working principle

The working principle of the cell sorting pack is the combination of forces experienced by the particles. Particles that flow in a spiral microchannel with a rectangular cross-section experience a combination of Dean drag forces and an inertial lift. The position at which particles of different sizes equilibrate depends on the ratio of these two forces.

This results in several particle streams that are size dependent, which can be extracted by designing appropriate outlets (see figure on the right).

Control flow rate with the benefits of responsive, pulse free flow

Pulse-free flow is critical for generating high quality and repeatable results in our cell sorting pack. The Flow EZ™ integrates the all-new DFC (Direct Flow Control) algorithm, which allows the user to set a flow rate directly on the instrument display. The applied pressure will automatically adjust to maintain the flow rate.

With the use of pressure instead of mechanical action, the Flow EZ™ gets a responsiveness ten times faster than syringe pumps. A low response time allows one to quickly execute operations such as stop flow and pressure/flow rate steps.