Microfluidic Size Cell Sorting Pack

[ESORT-PCK01]

    A complete cell sorting pack for starting size sorting experiments

    Why our cell sorting pack? Sorting and separating cells from a heterogeneous cell mixture is a common research application. The expanding need to isolate rare cells increases the demand for cell sorting devices.

    Microfluidic cell sorting can be combined with additional fluidic operations for complete lab on-a-chip applications and for diagnostic and therapeutic purposes. These devices use a wide range of techniques to sort cells with specific speeds and efficiencies.

    The microfluidic cell sorting pack allows sorting cells or particles according to their size using inertial and Dean drag forces. Fluigent has partnered with microfluidic ChipShop to provide a solution with well-designed microfluidic devices and excellent flow control for sorting experiments.

    cell sorting microfluidic pack
    Main benefits
    • Biocompatible
      Label-free

      No labeling steps

    • droplet monodispersity
      High-throughput

      Up to 3 mL/min

    • adaptable
      Passive sorting

      No external source required

    • Flexible

      Up to 8 cells/particles sizes sorted per experiment

    Features of the cell sorting pack

    Complete system

    This package includes all the components necessary to start cell sorting experiments.

    Engineered solution

    We built the package with quality pressure controllers, microfluidic chips, tubing and fittings to provide the most optimized and versatile system.

    Customization possible

    We can adapt the package to fulfil your needs.

    Cell sorting working principle

    Sorting working principle

    The working principle of the cell sorting pack is the combination of forces experienced by the particles. Particles that flow in a spiral microchannel with a rectangular cross-section experience a combination of Dean drag forces and an inertial lift. The position at which particles of different sizes equilibrate depends on the ratio of these two forces.

    This results in several particle streams that are size dependent, which can be extracted by designing appropriate outlets (see figure on the right).

    Control flow rate with the benefits of responsive, pulse free flow

    Pulse-free flow is critical for generating high quality and repeatable results in our cell sorting pack. The Flow EZ™ integrates the all-new DFC (Direct Flow Control) algorithm, which allows the user to set a flow rate directly on the instrument display. The applied pressure will automatically adjust to maintain the flow rate.

    With the use of pressure instead of mechanical action, the Flow EZ™ gets a responsiveness ten times faster than syringe pumps. A low response time allows one to quickly execute operations such as stop flow and pressure/flow rate steps.

    cell sorting high stability response time

    Main products of the package

    Specifications

    Fluigent package

    LineUp Flow EZ pressure controller 2 bar x1
    LineUp LINK Module (software control) x1
    Spiral sorter chips Fluidic 382 from microfluidic ChipShop (x3)
    P-CAP x1
    Fluigent connectors and tubing kit x1

    OxyGEN

    Control in real-time, protocol automation, data record and export
    ver. 1.0.0.0 or more recent

    See the offer


    Software Development Kit

    Custom software application
    ver. 21.0.0.0 or more recent

    See the offer

    microfluidic cell sorting schematic