Webinar-Spheroid Encapsulation in Alginate Microbeads Using Microfluidics
Encapsulating cells in alginate microbeads provides controlled microenvironments for multicellular spheroid formation and advanced 3D cell culture.
In this webinar, our experts present a microfluidic method to generate highly monodisperse alginate microbeads. Using a non-embedded co-flow focusing microfluidic setup, this approach forms double emulsions with an alginate core and a sacrificial oil shell, enabling uniform gelation and high cell viability.
This strategy allows reproducible bead production without complex chip engineering, making microfluidic spheroid encapsulation accessible to standard biology laboratories.
Join this session to learn how microfluidics can enable monodispersed alginate microbeads, high cell viability, and controlled spheroid growth in 3D environments.
Session
📅 Two live sessions available on March 31st :
• 10:00 AM CEST – Register here
• 4:00 PM CEST – Register here
Speaker :
- Dr. Joseph Farah
Microfluidic Application Engineer – Fluigent - Dr. Adrien Dewandre
Technology Lead, Emulsification & Droplet Generation – Secoya Technologies - Dr. Leon Rembotte
BioImaging and OptoFluidics, Laboratoire Photonique, Numérique et Nanosciences, Université de Bordeaux, IOGS & CNRS
What You Will Learn
✅ How to produce highly monodisperse alginate microbeads using microfluidic droplet generation
Discover the principles of microfluidic bead formation and how RayDrop technology enables robust and reproducible droplet generation.
With Dr. Adrien Dewandre
✅ How precise flow control improves microbead size and reproducibility
Learn how pressure-based flow control allows accurate tuning of droplet size and stability across a microfluidic setup.
With Dr. Joseph Farah
✅ A novel microfluidic method for spheroid encapsulation in alginate microbeads
Explore a practical strategy for producing homogeneous alginate beads using double emulsions with a sacrificial oil shell, enabling efficient gelation and recovery of microbeads suitable for multicellular spheroid formation.
With Dr. Leon Rembotte
✅ Interactive Q&A Session
Ask your questions and discuss your experimental challenges directly with our experts.
Wide-field fluorescence images of Live/Dead stained HEK293T cells at 2, 6 and 10 days after encapsulation. Scale bars: 100 μm. (Lab Chip 2026, 26 (3), 711–724)
Curious to find out more on our solution?
The Flow EZ™ is the most advanced system available for pressure-based flow control.
- A response time ten times faster compared to syringe pumps.
- Control flow rate with the benefits of responsive, pulse-free flow
The Complex Emulsion Production Platform is a ready-to-use platform to perform emulsification processes such as single emulsions and double emulsions.
- Save time with an integrated, organized, ready-to-use platform
- Get monodispersed complex emulsions rapidly (down to 2% monodispersity)
- Produce microparticle and microcapsule for encapsulation of API or other reagents inside different materials such as polymers (PLGA, UV polymerized materials), hydrogels and more.
The RayDrop Double Emulsion Device specific design allows for multiple liquid type emulsification within the same device with no coating needed. The device can create both single and complex emulsions.
- Droplet size from 25 µm to 450µm outer diameter.
- High monodispersity (CV < 2%)
- Water-in-oil-in-water (w/o/w) and oil-in-water-in-oil (o/w/o) within the same device
👉 Ready to improve your droplet generation system? Get the best of our expertise.
Why Explore Double Emulsion Microfluidics
Double emulsion microfluidics—defined as a process of encapsulating droplets within other droplets—allows for a higher level containment, encapsulation, compartmentalization, and controlled delivery of biological samples or templates. This technique proves useful in drug delivery, single cell studies, material development, or even formulating food and cosmetics.
With precise control over release dynamics, core size, and shell thickness, double emulsions serve as an adaptive basis for creating complex engineered systems designed to specific requirements.
Interested in learning more? Discover key reviews covering into the potential and techniques behind double emulsion microfluidics
- Microfluidics White Papers
An exploration of Microfluidic technology and fluid handling
Read more - Microfluidics White Papers
Droplet-based Microfluidics – A Complete Guide
Read more - Expert Reviews: Basics of Microfluidics
10 Tips for Reliable Droplet Generation
Read more - Expert Reviews: Basics of Microfluidics
Microfluidics in Drug Delivery: A New Era of Precision Medicine
Read more
- Microfluidic Application Notes
PLGA nanoparticle synthesis using 3D microfluidic hydrodynamic focusing
Read more - Microfluidic Application Notes
A quick and efficient double encapsulation method for FACS-based droplet sorting
Read more - Microfluidic Application Notes
Alginate Microbeads Production
Read more 
Webinar: Controlled UV-crosslinked microcapsule production using microfluidic technology
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