Electrical Impedance Spectroscopy Pack

    A complete system to start EIS experiments

    Electrical impedance spectroscopy (EIS) is a technique that guarantees fast throughput and label-free sensing so that it can be used to characterize electrical properties, size, or morphology of cells in microfluidic devices. Fluigent and Zurich Instruments drew on their expertise to propose a complete solution for EIS in microfluidics.

    Main benefits
    • Save time

      Label-free analysis

    • Flexible

      Wide range of analysis tools

    • Cost-effective

      Small sample volumes

    Features of Electrical Impedance Spectroscopy Pack 

    Microfluidic EIS

    Good integration with other analysis methods (e.g. optical detection), the EIS instruments allow for simultaneous multi-frequency measurement.

    Complete system

    With this package, you have all the fluidic components necessary to start EIS experiments.

    Engineered solution

    We built the package with the right pressure controllers, and microfluidic chips.

    Customization possible

    We can adapt the package to fulfill your needs.

    Working principle

    Electrochemical impedance spectroscopy is a well-established method that has been applied in many fields of science and technology. In the last decade, EIS has also been used extensively in biochemical and medical applications, as it has proven to be a sensitive technique to detect and measure various biochemical or biological events.

    For example, it can be used for sensing the formation of antigen–antibody complexes, immunosensing, DNA characterization or detection of DNA hybridization, as well as characterizing living cells.

    Thus, the EIS technique combined with microfluidics, micromachining, and Microelectromechanical systems (MEMS) techniques, demonstrated that it can be a very useful and valuable tool in biochips for easy and fast characterization of bio-samples, which is especially important as until now few automated methods exist for such purposes. Moreover, another essential merit of EIS is that it is label-free, therefore simplifying even further the preliminary sample preparation for analysis.

    The microfluidic EIS technique involves monitoring the change in the frequency-dependent dielectric properties of the channel as the cell passes through it. The measurement requires both high sensitivity and fast response.

    Electrical impedance spectroscopy principle
    Electrical impedance spectroscopy principle

    As the analyte enters and exits the differential electrode pairs in a microfluidic chip, a peak and a trough in current are observed. With a differential input, the signal from the surrounding fluid is suppressed, meaning each cytometry event can be resolved with reduced noise.

    Continuous flow microfluidic devices with embedded microelectrodes for electrical measurements can be employed for detecting and classifying single cells or particles (e.g. beads or droplets) in a high throughput manner. Further, since the dielectric properties of a biological cell are defined by its cellular characteristics such as cell volume, composition and architecture, impedance spectroscopy can be used to differentiate between cell types. When using impedance spectroscopy, the frequency response of the cell can be measured and fitted to an equivalent circuit model. This allows quantitative measurements related to the different properties of the cell to be extracted, such as membrane thickness and cytoplasm conductivity. Moreover, it is also possible to perform precise droplet analysis using EIS (droplet size, counting, cell-in-droplet quantification).

    Han, Z. et al (2020) proposed a microfluidic impedance flow cytometry device with a constriction microchannel for simultaneously characterizing the mechanical and electrical characters of plant cells at the single-cell level. 

    Particularly, by using the Electrical Impedance Spectroscopy Pack together with impedance analyzers from Zurich Instruments, two characteristic parameters, passage time and impedance opacity, were extracted as biophysical parameters to specify the deformability and membrane electrical property of single plant cells, respectively. 

    Taken together, this study provides a high-throughput system for rapid and sensitive biophysical characterization of plant cells at the single-cell level and it also envisions the development of more powerful biosensors for single cell phenotyping, which could complement the traditional approaches to obtain a more comprehensive view of gene functions.

    The Importance of Pressure-Based Flow Control

    Control flow rate with the benefits of responsive, pulse free flow

    Pulse-free flow is critical for generating high quality and repeatable results. The Flow EZ™ integrates the all-new DFC (Direct Flow Control) algorithm which allows the user to set a flow rate directly on the instrument display. The applied pressure will automatically adjust to maintain the flow rate.

    A response time ten times faster compared to mechanical solutions

    With the use of pressure instead of mechanical action, the Flow EZ  gets responsiveness ten times faster than syringe pumps. A low response time allows one to quickly execute operations such as stop flow and pressure/flow rate steps.

    Selected publications from our customers


    LineUp Flow EZ pressure controller (2000 mbar) x2
    LineUp LINK Module (software control) x1
    FLOW UNIT M (x1) and L (x1)
    Electrical impedance spectroscopy chip and holder (contact us)
    HF2LI from Zurich Instruments
    HF2TA from Zurich Instruments
    Kit including all necessary tubing & fittings


    Control in real-time, protocol automation, data record and export
    ver. or more recent

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    Software Development Kit

    Custom software application
    ver. or more recent

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    Selected publications from our customers