The Raydrop: A new droplet generation device based on non‑embedded co‑flow‑focusingRead more
After introducing the Raydrop, Adrien Dewandre and his team from ULB demonstrate that this new configuration offers the ability to emulsify any liquid with a wide range of droplet sizes. This universality is demonstrated with experimental results. Modeling is also performed, supporting experimental results, and allowing for the prediction of droplet size and production regime (dripping, jetting regimes).
High throughput single cell analysisRead more
Individual cell heterogeneity within a population has invalidated historic classification methods based on macroscopic considerations and given rise to new evaluation techniques based on single cell transcriptional signature. In this context, thanks to high throughput screening capacities, easy fluid handling and reduced costs related to device miniaturization, microfluidics has emerged as a powerful tool for single cell manipulation and analysis.
How to reproduce active biomimetic stimulation in vitro?Read more
Organ-on-a-chip (OOC) technology has paved the way for investigating the impact of mechanical strain in cell biology research by reproducing key aspects of an in vivo cellular microenvironment. Combining microfluidics and microfabrication enables one to reproduce mechanical forces experienced by living tissues at the cell scale.
Passive mechanical stimulation induced by laminar and pulsatile shear stressRead more
Indirect mechanical forces and shear stress are integral parts of the cellular microenvironment. Reproducing these mechanical forces and shear stress is critical to capture the physiology of living tissues. Three types of flows are typically generated for producing shear stress : laminar, pulsatile, or interstitial flow.
Organoid Culture in micro-beadsRead more
Microbead-based microfluidics is a powerful technique that generates highly monodispersed picoliter-sized beads into a continuous phase. This method has been successfully adapted to cell culture to encapsulate cells in micron size biocompatible hydrogel beads.
In vivo, most cells are constantly exposed, actively or passively, to mechanical forces. Reproducing these physiological constraints in vitro is essential to induce the right phenotype to cells, finalize their maturation and maintain homeostasis. The wide range of pressure covered by Fluigent products permits one to accurately study biomechanics from molecular level to organ scale.
Biochemical EnvironmentRead more
Cells are constantly exposed to biochemical stimulation from the early embryonic stage to adult life. The spatiotemporal regulation of these signals is essential as it determines cell fate, phenotype, metabolic activity as well as pathological behaviors. The fast response and high stability of Fluigent instruments make them the best solution available on the market to reproduce these complex variations in vitro.
How to ask cells what proteins they produce?Read more
The conventional way to isolate cells is by using Fluorescence Activation (FACS) to sort them into barcoded well plates (96 wells, 384 or 1536). This step is quite fast with the FACS currently available, but segregating thousands of cells into plates will result in a long process of pipetting. Droplet microfluidics allows for fast and low volume compartmentalization up to a thousand drops produced per second
Funded research program participationRead more
Many national and internationally funded projects bring together science and innovation. These projects cover a large number of subjects. Fluigent takes part in several European and French funded programs to provide expertise and resources in microfluidics and related applications.