Sample injection and recirculation microfluidic valve for industry

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    Fluigent L-X

    The L-X is a bidirectional 6-port/2 position microfluidic valve. It is designed for precise sample injection or fluid recirculation in cell culture applications.

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    Main benefits
    • Quick

      Fast valve actuation time (< 100 ms)

    • Accurate

      Low internal volume

    • compact
      Compact

      Designed for system integration

    • Biocompatible
      Biocompatible

      Chemically and biologically compatible

    Features

    Precise fluid injection

    Several sample loops are available, from 5 µL to 100 µL.

    A versatile valve

    The valve can be operated using pressure up to 7 bar (100 psi), and the wetted material is PEEK, a highly chemically and biocompatible material.

    Fluigent software for automation

    The valve can be controlled by Fluigent software for long-term experiments. Create a time-based protocol to set actuation timing of the valve(s).

    Minimized liquid waste

    Low port-to-port volume of 660 nL

    Related applications

    recirculation valve

    Working principle

    Peripheral ports (numbered from 1 to 6) can alternatively be connected the right or the left channels. The L-X is actuated by a motor that drives a rotor – where the fluidic path is engraved – against a stator – containing the fluidic paths.

    Fluid recirculation

    The L-X can be used as a useful cell culture tool: a small volume of buffer can be recirculated within a closed loop into the chip for several hours or days. Combined with our pressure-based flow controllers, it can achieve a highly stable flow rate, allowing to better mimic flow-induced shear stress experienced by cells in vivo.

    recirculation microfluidic
    precise fluid injection microfluidic

    Precise fluid injection

    The L-X can be used as a useful cell culture tool: a small volume of buffer can be recirculated within a closed loop into the chip for several hours or days. Combined with our pressure-based flow controllers, it can achieve a highly stable flow rate, allowing to better mimic flow-induced shear stress experienced by cells in vivo.

    Specifications

    PERFORMANCE

    Pressure ratingUp to 7 bar (100 psi)
    Stability0,1% on the measured value (effective beyond 10% of the maximum pressure)
    Response time100 ms
    Step0,03% of the maximum pressure

    HARDWARE SPECIFICATIONS

    Dimensions70 x 90 x 150 mm
    Weight475 g
    Internal volume660 nL

    ELECTRONICAL SPECIFICATIONS

    Power supplyRJ45

    CHEMICAL COMPATIBILITY

    Gas compatibilityDry, oil-free gas, air, any non corrosive or non explosive gas
    Liquid compatibilityAqueous solvent, oil, organic solent, biological sample
    wetted materialsPEEK

    Fluigent pressure-based flow controllers VS Peristaltic pump

    Fluigent pressure-based flow controllersPeristaltic pumps
    Flow stabilityHigh (<1% variation)Low (>20% variation)
    Response timeHighMedium
    Pressure controlYesNo
    PriceMedium-HighLow
    ImplementationRequires pressure sourcesEasy

    OxyGEN

    Control in real-time, protocol automation, data record and export
    ver. 1.0.0.0 or more recent

    See the offer

    Software Development Kit

    Custom software application
    ver. 21.0.0.0 or more recent

    See the offer

    How to add the L-X in my experiment and how to connect it?

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    • Connect the L-X to the SWITCH EZ port
    • Cut the 1/16’’ OD tubing to the desired length, leaving a square-cut face.
    • Insert the tubing into a nut until it passes 1.5 – 3mm.
    • Insert into a port of L-X, twist until it is tightened. You can pull gently the tubing to verify that it is securely connected to the port.

    1.   HOW TO CONNECT A SAMPLE LOOP TO THE L-SWITCH™?

    How to connect a sample loop to the L-X?

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    • In order to connect a sample loop to the L-SWITCH™, you will need two nuts and two ferrules, in addition to the sample loop itself (nuts and ferrules are provided with the sample loop).
    • Pass one end of the sample loop through a nut.
    • Add a ferrule
    • Hold the set together and insert it into a port of the L-SWITCH™ (for example, port 1 here).
    • Twist until the set is tightened. You can pull gently the sample loop to verify that it is securely connected.
    • Repeat these steps for the other end of the sample loop to connect it to another port of the L-SWITCH™ (for example port 4 here).

    How can I clean the L-X after use?

    You can clean the L-Switch™ in the same way than the Flow Unit.

    Besides, Fluigent strongly advises you to use filtered solutions. Wetted materials are PEEK only.

    Expertise & resources

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