Webinar: RBC Mechanics and Channel Activation by Micropipette Aspiration
Role of Piezo1 Channel Activity in RBCs and Its Link to Alzheimer’s Disease
Join our webinar to learn about cellular micropipette aspiration, a precise method for measuring mechanical properties and mechanosensitive channel activation in individual cells using controlled pressure and real-time monitoring.
This session focuses on red blood cells, assessing key parameters including activation pressure, Young’s modulus, membrane tension, and viscosity. We’ll also discuss the application of the technique in studying red blood cell changes linked to Alzheimer’s disease.
Speakers
Dr. Dan Cojoc
CNR-IOM Materials Foundry Institute of of the National Research Council of Italy
Dr. Joseph Farah
Microfluidic Application Engineer – Fluigent
Agenda :
- Introduction: Single-Cell Mechanics
The importance of measuring mechanical properties at the single-cell level and its impact on understanding cell behavior.
- Micropipette Aspiration Technique
Learn how Fluigent’s method for precise cell manipulation and characterization using controlled pressure.
Speaker: Dr. Joseph Farah
- Applications in Red Blood Cells and Alzheimer’s Disease
A case study on RBC mechanics and mechanosensitive channel activation, highlighting relevance to Alzheimer’s disease research.
Speaker: Dr. Dan Cojoc
- Interactive Q&A Session
Have questions or challenges? Join our live Q&A session, where our speakers will provide solutions and insights.
Live session: September 25th at 4:00 PM CEST (Paris time)
- Boston, USA → 10:00 AM EDT
- Toronto, Canada → 10:00 AM EDT
- London, UK → 3:00 PM BST
- Chicago, USA → 9:00 AM CDT
- Los Angeles, USA → 7:00 AM PDT
Register now to attend live or receive the recording if the time isn’t convenient.
About the Speaker:
Dr. Dan Cojoc – CNR-IOM (Italy)
Dr. Dan Cojoc is a Senior Scientist at the Institute of Materials of the National Research Council of Italy (CNR-IOM). He has a background in Optical Engineering and Technical Physics, with over 30 years of experience in optics, nanoscience, and biophotonics. He has developed numerous advanced microscopy and spectroscopy systems, including more than 20 optical tweezers setup integrated with microfluidics and other techniques. His research has been widely published, with over 150 international papers and several patents in the fields of nanotechnology and biophotonics.
Currently, Dr. Cojoc focuses on applying these optical tools to study cell biology, specifically cell mechanics and mechanotransduction. His expertise extends to using micropipette aspiration and related methods to investigate the mechanical properties of red blood cells, contributing important insights into their function in health and disease.
A complete setup for starting micropipette aspiration experiments
The package allows one to manipulate and characterize cells at the single-cell level. It is a powerful non-invasive technique to evaluate how the biomechanical properties of single cells or tissues govern cell shape, cell response to mechanic stimuli, transition from nontumorigenic to tumorigenic state, or morphogenesis.
