High monodispersity (2%)
PLGA Microparticle Production Pack (Standard Pack)
[1DPPL01]Produce monodispersed PLGA microparticles
The PLGA microparticle production station is a robust, high performance solution to generate polymer microparticles in a homogenous and fully controlled manner. The performance brought by the RayDrop droplet generator, with the combination of poly(lactic-co-glycolic acid) as an encapsulation polymer and ethyl acetate as a solvent provide a biocompatible solution lowering both hazard risk and precipitation time. Suitable for biological applications, the RayDrop and its station offer a semi-automated solution for one of the most successful drug delivery systems in laboratories and clinics. To solve the current problems of the PLGA microparticles synthesis, droplet based microfluidics appears to be a powerful tool. Droplet control and generation allows highly monodispersed and continuous production as compared to batch emulsion methods.
The standard pack offers the possibility to generate PLGA microparticles manually by using the potential of the RayDrop device and the fluid control precision and stability with Fluigent instruments.
Main benefits
Repeatability- Flexibility
Control on particle size
Ease of useStraight forward set up
Features
A complete system
With this package, you have all the components necessary to start generating PLGA droplets
An engineered solution
We built the package with the right pressure controllers, microfluidic chips, valves to make sure you have the highest flexibility in terms of droplet size and generation rate
A dedicated protocol
A protocol is available to assist you setting up and starting your experiments
Customization possible
We can adapt the package to fulfill your needs (droplet size, generation rate)
Related applications
Main products of the package
Specifications
PARTICLE PRODUCTION
| Dispersed phase | PLGA lactide: glycolide (75:25), mol wt 66,000-107,000 |
| PLGA concentration used | 2%, 5% ans 10% |
| Continuous phase | Ethyl acetate |
| Doplet size range | 60µm to 120 µm |
| Particle size range | 20µm to 50 µm |
| Production rate | Up to 60mg/h |
| Production frequency | U to 1000Hz |
| Monodisperity | 2% |
FLOW CONTROL
| Pumps | Fluigent Flow EZ™ (2000mbar) |
| Flow sensors | Fluigent FLOW UNIT (M and L) |
| Automated valves | Fluigent 2-SWITCH™ |
IMAGING
| Microscope | Fluigent Digital high-speed microscope |
OxyGEN
| Control in real-time, protocol automation, data record and export |
| ver. 1.0.0.0 or more recent |
Imaging
| Pixelike Capture Software |
| Traditional methods (Batch methods) | Fluigent PLGA microparticle production station | |
| Particle size distribution | ~20% | ~2% |
| Reproducibility | Low | High |
| API mixing | Uneven | Uniform |
| Live particle size control | No | Precise |
| Continuous / In line production | No | Yes |
| Microfluidic methods available on the market | Fluigent PLGA microparticle production station | |
| Particle size distribution | ~5% | ~2% |
| Semi automated production | No | Yes |
| Ethyl acetate dedicated protocol | No | Yes |
| Device regeneration | No (glass chip changed when clogged) | Yes (the RayDrop can be maintained) |
| Connectors | Non standard, user dependant quality (leakage, blockage) | Standard fittings for better sealing |
Can I use a polymer other than PLGA?
The method has been designed for PLGA. Changing polymers may change the physical fluid properties and lead to different results.
How can I prevent the clogging from PLGA?
Follow the procedure detailed in the application note. Please note the priming and cleaning procedures.
The nozzle (or capillary) sems clogged with PLGA. How can I fix it?
If the usual cleaning procedures from the good practice guide cannot unclog the nozzle, contact customer support for help.
Some of the ethyl acetate has flowed into the continuous phase chamber. What should I do?
If some ethylacetate without PLGA has flowed into the chamber it can be flushed out easily.
Follow the instructions in the good practice guide.
I can see a drop of ethlacetate fixed on the outside of the nozzle. How can I get ride of it?
If some ethylacetate without PLGA is fixed around the nozzle, it can be easily flushed out.
Follow the instructions in the good practice guide.
Some of the PLGA solution has flowed into the chamber. What shoulg I do?
If some PLGA gets into the chamber, it has to be eliminated quickly.
Refer to the good practice guide for the exact procedure.
Air bubbles regularly appear during the experiment. What should I do?
If air bubbles appear during experiment we advise one to switch to the ethyl acetate solution (if you are in the PLGA configuration) and let it flow for a minute to remove all PLGA from the system.
Then make sure that all connectors are tightened properly.
Refer to the application note procedure to avoid air bubble infiltration.