Cell perfusion pack - High Throughput

    The perfect set for high throughput cell perfusion/Organ on chip perfusion.

    Fluigent has joined with BEOnchip to offer complete solutions for organ-on-chip applications combining innovative, robust, and easy-to-use chips with high-performance instruments.

    This cell perfusion pack has been designed for high throughput experiments. The compactness and arrangement of the different elements have been optimized to perfuse a maximum number of chips and then grow multiple organ models at the same time in an incubator. It includes an 8-channel compact pressure controller, a flow rate platform, and reservoir holders directly adapted on an incubator trail. This setup, which has a very intuitive interface, will mimic physiological conditions, perform long-term experiments without additional effort, and produce high reproducibility and scalability.

    The cell perfusion pack will allow users to control both pressure and flow rate, switch between media and drugs, obtain a stable and pulseless flow rate, have a large range of flow rates, and customize and create complex patterns.

    Cell perfusion pack
    Main benefits
    • Biocompatible
      Designed for cell biology

      Contamination-free, sterile

    • Easy to use

      Simple connections

    Introduction to our Cell perfusion pack.

    The growth of cells requires the synergetic effect of various complex external and internal environmental factors. Hence, the reality of the external environmental parameters is to be considered in the establishment of in vitro physiological models. By combination of microfluidic technology, micromachining and cell biology, environmental parameters can be precisely controlled on organs-on-chips, which may in turn generate fluid shear stress, mechanical stress, biochemical concentration gradients and other physical and chemical stimuli. The cells on the chip can respond to these stimuli, develop self-organization and express more realistic physiological functions, exhibiting special advantages of organ-on-chip in the establishment of an in vitro physiological model.

    Features of the Cell Perfusion Pack

    Optimized seeding

    Cell seeding in 3D hydrogel is a delicate manipulation. Hydrogels are fragile and soft and can easily fracture or detach from the chip if the flow is unstable.

    Manual cell seeding is not recommended as the discontinuous flow results in heterogeneous cell seeding and can crack the hydrogel.

    The manual mode of the Flow EZ is perfect to circumvent these limitations as it allows the implementation of small flow rate increments while keeping an eye on the microscope.

    Stable & complex flow patterns 

    Fluigent’s products have unprecedented performances in terms of stability and responsiveness.

    High stability is particularly useful for applications with constant shear stress like vascular perfusion.

    High responsiveness is essential to reproduce complex flow patterns like aortic pressure variations. For a complex flow pattern, the orders can be set in flow or pressure variations in the cell perfusion pack.

    Protocol automation

    Once the parameters are set and optimized, protocol automation is the key to saving time, limiting contamination, and decreasing variability.

    For any protocol, valves; pressure; and flow controller from Fluigent can be assembled to automate the protocol using user-friendly software (OxyGEN).

    Customization possible

    Fluigent can adapt the package to fulfil your needs based on  specific flow rates and/or pressure.

    Related applications

    Main products of the cell perfusion pack

    cell perfusion pack set up

    Organ-on-a-Chip Perfusion Pack: Applications

    Cell culture under flow

    Cultivating cells under flow perfusion has two main advantages: constant medium renewal and reproduction of mechanical strain in vitro. To grow cells under perfusion using a cell perfusion pack, we recommend using the BE-Flow chip.

    Read more

    cell perfusion chip
    cell perfusion microfluidic chip

    Chemotaxis

    Cells are constantly exposed to biochemical stimulation from the early embryonic stage to adult life. The spatiotemporal regulation of these signals is essential as it determines cell fate, phenotype, metabolic activity as well as pathological behaviors. Biochemical stimulation is also central in oncology as it drives the migration and expansion of malign tumors.

    To study chemotaxis in 3D hydrogel we recommend using BE-GRADIENT.

    Read more

    Co-culture under perfusion

    Co-culture under perfusion is essential for various applications for the following reasons:

    • To fully differentiate given cell types, coculture with another cell type is required
    • In cosmetics or drug testing to reproduce interfaces link skin, lung or gut and monitor the uptake of active molecules
    • To reproduce liquid-liquid or air-liquid interfaces.

    The BE-Doubleflow chip best fit applications in organ-on-chip cell perfusion as a porous membrane separates the central chamber into two channels.

    Read more

    cell perfusion for co-culture
    Transwell under flow cell perfusion pack

    Transwell under flow

    Transwells are classic devices widely used in standard cell culture to co-culture cell types, reproduce 2D-2D interfaces, 3D-2D interfaces, study cell invasion, assess monolayer permeability or active molecule uptake.

    However, Transwell devices use large volumes of liquid, and communication is therefore slow and low concentration, signalling factors are diluted, which altogether hampers studying cellular communication. Furthermore, the culture is entirely static, which precludes emulation of dynamic processes and the application of controlled cell biochemical and/or physical stimuli.

    Using a microfluidic format can solve some of these issues by offering sub-milliliter volumes, dynamic culture, and exquisite spatiotemporal control over physical and chemical parameters in the cell/tissue vicinity.

    For this application, we recommend the BE-Transflow

    Read more

    Cell culture with regulated Shear Stress

    Mechanical forces are potent regulators of cellular structures and functions in both health and disease. As a result of their unique location, endothelial cells experience several mechanical forces.

    Of these forces, shear stress is particularly important as it stimulates the release
    of vasoactive substances and changes gene expression, cell metabolism, and cell morphology.

    To grow cells under regulated shear stress, we recommend the BE-FLOW

    Read more

    flow-induced shear stress cell perfusion pack

    Specifications

    LineUp Flow EZ pressure controller
    LineUp LINK Module (software control)
    BEOnChip microfluidic chip
    Fluigent connectors and tubing kit

    OxyGEN

    Control in real-time, protocol automation, data record and export
    ver. 1.0.0.0 or more recent

    See the offer


    Software Development Kit

    Custom software application
    ver. 21.0.0.0 or more recent

    See the offer

    Connect the chip to the microfluidic system


    Switching from static culture to fluid perfusion

    Expertise & resources

    Related products

    Accessories