BE-DoubleFlow

[OOC-DBLE-08]

    Explore the crosstalk between different 2D and 3D cultures

    BE-Doubleflow is Beonchip’s most advanced device. It consists of two perfusable channels connected via a porous membrane. Explore the crosstalk between different 2D and 3D cultures in a biomimetic environment and control the efficiency of the interaction by selecting the optimal pore size for your application. This is the optimal device when a hypoxia environment is needed, for studying the effect of circulating particles (bacteria, immune system, circulating tumor cells) and for endothelium/epithelium barrier when no ALI is needed or when flux plays a role in both sides of the coculture.

    Main benefits
    • Easy to use

      Simple fitting & connections

    • Biocompatible
      Biomimetic environment

      2D and 3D culture

    • Sterile

      Dedicated to cell biology

    Features

    Easy to use

    Be-Doubleflow is compatible with any type of optical microscopy and its slide format has been chosen for easy handling under a microscope.

    Easy to connect

    Be-Doubleflow is compatible with all Fluigent pressure-based flow controllers

    No unspecific absorption

    Unlike in other PDMS devices, Be -Doubleflow is made of lipophobic thermoplastic materials and does not present unspecific drug absorption issues. It allows immunohistochemistry with fluorescent detection

    Cell recovery

    The cell cultures used in the Be-Doubleflow can be easily recover for further experimentation.

    Related applications

    Culture models

    Explore the crosstalk in the endothelium epithelium barrier under flow in both channels of the device, mimicking a more physiological environment for the study of different organs such as kidney, liver, gut, or heart, among others.

    Combine 3D and 2D cultures in a hypoxia environment where oxygen is supplied to the cells via the medium flowing in one of the channels.

    Research the effect of circulating particles such as circulating tumor cells, immune system cells, bacteria, fungi, viruses, and many more in single cell culture, or in a complex coculture.

    Applications

    BE-Doubleflow allows endothelium/epithelium barrier coculture where a hypoxia environment is needed or when flux plays a role also in the epithelium culture (Kidney, liver, heart, lung, gut…). In addition, its two perfusable channels offer a perfect environment for studying the effect of circulating particles (bacteria, immune response, circulating tumor cells).

    Example applications

    Kidney on chip: The coculture of HPTC cells and vascular endothelial cells clearly improves the performance of the HPTCs and increases the maintenance of the renal epithelia in vitro. Using BE-Doubleflow it is possible to coculture both types of cells simultaneously, circulating urine on the renal epithelium culture and blood-like medium in the vascular culture.

    Specifications

    PERFORMANCE

    HeightWidthLengthTotal volume
    Each channel375 µm1.5 mm45 mm31.2 µL
    Inlet/outlet7 mmUNF 1/4″ – 28 UNF 1/4″ – 28 130 µL
    Medium reservoir5 mm3.6 mm8.8 mm185 µL
    Membrane pore size1 µm 1 µm 1 µm 1 µm

    Getting Started

    Coating and cell culture

    Expertise & resources

    Related products

    Accessories