Simple fitting & connections
Microfluidic chip for 2D and 3D cultures
[OOC-DBLE-08]BE-DoubleFlow
Explore the crosstalk between different 2D and 3D cultures.
BE-Doubleflow is Beonchip’s most advanced device. It consists of two perfusable channels connected via a porous membrane. Explore the crosstalk between different 2D and 3D cultures in a biomimetic environment and control the efficiency of the interaction by selecting the optimal pore size for your application. This is the optimal device when a hypoxia environment is needed, for studying the effect of circulating particles (bacteria, immune system, circulating tumor cells) and for endothelium/epithelium barrier when no ALI is needed or when flux plays a role in both sides of the coculture.
Main benefits
Easy to use- Biomimetic environment
2D and 3D culture
- Sterile
Dedicated to cell biology
Features
Easy to use
Be-Doubleflow is compatible with any type of optical microscopy and its slide format has been chosen for easy handling under a microscope.
Easy to connect
Be-Doubleflow is compatible with all Fluigent pressure-based flow controllers
No unspecific absorption
Unlike in other PDMS devices, Be -Doubleflow is made of lipophobic thermoplastic materials and does not present unspecific drug absorption issues. It allows immunohistochemistry with fluorescent detection
Cell recovery
The cell cultures used in the Be-Doubleflow can be easily recover for further experimentation.
Related applications
Culture models
Explore the crosstalk in the endothelium epithelium barrier under flow in both channels of the device, mimicking a more physiological environment for the study of different organs such as kidney, liver, gut, or heart, among others.
Combine 3D and 2D cultures in a hypoxia environment where oxygen is supplied to the cells via the medium flowing in one of the channels.
Research the effect of circulating particles such as circulating tumor cells, immune system cells, bacteria, fungi, viruses, and many more in single cell culture, or in a complex coculture.
Applications
BE-Doubleflow allows endothelium/epithelium barrier coculture where a hypoxia environment is needed or when flux plays a role also in the epithelium culture (Kidney, liver, heart, lung, gut…). In addition, its two perfusable channels offer a perfect environment for studying the effect of circulating particles (bacteria, immune response, circulating tumor cells).
Example applications
Kidney on chip: The coculture of HPTC cells and vascular endothelial cells clearly improves the performance of the HPTCs and increases the maintenance of the renal epithelia in vitro. Using BE-Doubleflow it is possible to coculture both types of cells simultaneously, circulating urine on the renal epithelium culture and blood-like medium in the vascular culture.
Specifications
PERFORMANCE
| Height | Width | Length | Total volume | |
| Each channel | 375 µm | 1.5 mm | 45 mm | 31.2 µL |
| Inlet/outlet | 7 mm | UNF 1/4″ – 28 | UNF 1/4″ – 28 | 130 µL |
| Medium reservoir | 5 mm | 3.6 mm | 8.8 mm | 185 µL |
| Membrane pore size | 1 µm | 1 µm | 1 µm | 1 µm |
Getting Started
Coating and cell culture