Up to 7092 pfs
Digital High-speed Microscope Camera
[1IMCA03]A dedicated microscope for microfluidic experiments
Microfluidic experiments often require visualization of events inside microchannels, thus requiring the use of microscopy. Generally, scientists need to control and monitor several devices at the same time, such as pumps (Flow EZTM), valves, sensors (Flow Unit) and a microscope. Fluigent’s high-speed microscope camera is connected with a USB 3.0 cable to a PC where the user can visualize their microfluidic device with the Pixelink Capture Software, together with other Fluigent Software (OxyGEN).
Main benefits
High-speed camera- Adapted for kHz rates
Visualize generated droplets and fast-moving particles
- Ready to use
Pre-assemble microscope and dedicated software
Features of Fluigent High-Speed Camera
A camera designed specifically for microfluidic experiments
The camera was selected to achieve image capture rates of up to 7028 fps, which is ideal for droplet experiments. High rates like these allow users to calculate frequencies of droplet generation and the encapsulation rate with image post-processing.
It is a compact and ready-to-use digital high-speed camera, delivered with software, support, LED, camera, lens (5x) and zoom lens (6.5x), providing high-quality optics.
The ideal lens and working distance
The selected lens is 5x with a 6.5x zoom including coaxial light, ideal for the dimensions used in microfluidics. In addition, a working distance of 34 mm allows users to work with all kinds of microfluidic setups.
A versatile software
Pixelink software offers many features including adjustable imaging settings (exposure time, brightness, contrast, and more), ROI selection and annotations, which are ideal for microfluidic experiments.
Fluigent support
Our team is dedicated to helping you get started with microfluidic experiments with this microscope.
Related applications
Example applications of droplet microfluidics
Water-in-oil and oil-in-water emulsions
An emulsion refers to a mixture of two liquids that are normally unable to mix together. It consists of small droplets of one liquid suspended within another liquid. Specifically, in oil-in-water (O/W) emulsions, tiny droplets of oil are dispersed and enclosed within the continuous water phase. In water in oil (W/O) emulsions, on the other hand, small droplets of water are dispersed within the continuous oil phase. These emulsion techniques, O/W and W/O are extensively utilized in various industrial and research settings to create droplets, hydrogel beads, polymer beads, or wax beads.
Fluigent has published several application notes on this topic, introducing a cutting-edge technology that involves the use of a high-speed microscope camera. This tool enables researchers to observe the production of high-quality monodispersed droplets.
Figure 1: Schematic fluidic setup.
Double Emulsions Generation
In addition to its use in single-emulsion experiments, the microscope for microfluidics is also utilized in setups involving double emulsions. A double emulsion refers to a droplet enclosed within a larger droplet consisting of immiscible phases. The creation of double emulsions holds great promise in various academic and industrial fields. Examples include fragrance manufacturing in the cosmetics industry, flavor manufacturing in food applications, encapsulation of food supplements like vitamins for taste masking, drug delivery in pharmaceutics (such as protection and controlled release of active pharmaceutical ingredients in microcapsules), and more.
The application note “Double Emulsion Generation” introduces Fluigent’s Double Emulsion Production Station, a comprehensive system equipped with the microscope. This system enables production of monodisperse double emulsions. It features the RayDrop, developed and manufactured by Secoya, to facilitate one-step production of double emulsions using a single device.
PLGA Microparticle Synthesis
As mentioned previously, Fluigent technology now enables production of highly monodispersed particles, ensuring reproducibility in drug release. One application of this technology involves using PLGA as a carrier for active pharmaceutical ingredients. To achieve a high level of uniformity, the RayDrop is utilized as part of a microfluidic setup that includes the microfluidics-compatible microscope camera.
The following application note describes a new platform and protocol that utilize ethyl acetate at various concentrations for PLGA polymerization and continuous synthesis.
Specifications Microscope 2MP
| Maximum field of view (maximum zoom) | 0.5 mm * 0.4 mm |
| Minimum field of view (minimum zoom) | 3.5 mm * 2.8 mm |
| Objective lens magnification | 5X |
| Zoom ratio | 6.5X |
| Working distance | 34 mm |
| Number of pixels per micron at max zoom | 2.56 pixel/micron (0.39 micron/pixel) |
| LED Luminous Flux | 145 lumens |
| LED color temperature | 6500K |
| Imaging device | 2.3 Megapixel monochrome camera |
| Sensor | ON Semi Vita 5000 |
| Resolution | 1920 x 1200 |
| Frame rate | 76 fps at max resolution/7092 fps at min resolution |
| Video image file type | .avi |
| PC connection | USB 3.0 |
| System dimension | L * l * h = 320 mm * 305 mm * 538 mm |
| Weight | 4925 g |
| Part Number | 1IMCA03 |