High control over bead size
Alginate Bead Generation Pack
[O-SE-ALG-PCK]A system for producing monodisperse alginate beads
The Fluigent Alginate bead generation pack is a robust and complete system for producing outstanding monodispersed Alginates beads. The generation pack is flexible, allowing the alginate beads to change particle sizes in hundreds of milliseconds without interrupting the production.
The generation pack is powered by Fluigent’s LineUP microfluidic pumps and the RayDrop device, a breakthrough technology for high-quality particles and beads production.
Main benefits
Flexible- Reproducible
High monodispersity (2%)
- Robust
Continuous production
Alginate microbeads are used in a wide range of therapeutic applications including encapsulation, therapeutics delivery, tissue engineering, regenerative medicine and in vitro cell culture. Some of the striking characteristics of alginate beads are their biocompatible nature, easy gelation and availability of open functional groups with ease of manipulation to synthesize alginate derivatives with advanced desirable properties. Alginate microbeads are recommended due to their nontoxicity and biodegradability, and also because they are formed from naturally occurring polysaccharides and can be made from a rapid preparation method.
The application note demonstrates that alginate beads can be successfully produced using our setup with precise control of droplet size.
Moreover, it’s possible to use different concentrations of alginate due to the great flexibility of our system, which allows users to change the nozzle in case of working with high viscosity liquids. Changing capillary size can be easily done by the user to target different droplet sizes.
Due to the precision and versatility of the Alginate bead generation pack, it’s possible to produce particles for various biological applications: encapsulation, drug delivery, stem cell culture, and tissue engineering scaffolds.
Alginate bead generation pack: Features
Complete system
This packages includes all the components necessary to start generating alginate droplets.
Engineered solution
The package was built with pressure controllers, microfluidic chips, and valves that are customizable in terms of droplet size and generation rate.
Dedicated protocol
A protocol is available to assist you in setting up and starting your experiments.
Customization possible
We can adapt the package to fulfil your needs (droplet size, generation rate).
Related applications
Main products of the Alginate bead generation pack
Specifications
Beads production
| Dispersed phase | Alginate + Calcium + EDTA |
| Alginate concentration | 0.5%, 1%, 1.5%, 2% |
| Continuous phase | dSurf |
| Particle size range | 50 – 250 µm |
| Production frequency | Up to 5 000 Hz |
| Monodispersity | 2% |
Flow Control
| Pumps | Fluigent Flow EZ (2 000 mbar) |
| Flow sensors | Fluigent Flow Unit (M) |
| Automated valves | Fluigent 2-SWITCH |
Imaging (optional)
| Microscope | Fluigent Digital high-speed microscope |
OxyGEN
| Control in real-time, protocol automation, data record and export |
| ver. 2.2.0.0 or more recent |
Software Development Kit
| Custom software application |
| ver. 22.2.0.0 or more recent |
| Extrusion method | Fluigent microfluidic method | |
| Particle size distribution | up to 50% | ~ 2% |
| Reproducibility | low | High |
| Particle size control | no | Precise |
| Continuous (in line) production | no | yes |
Microfluidics for Alginate Microbeads Production