High throughput single cell analysis
Individual cell heterogeneity within a population has invalidated historic classification methods based on macroscopic considerations and given rise to new evaluation techniques based on single cell transcriptional signature. In this context, thanks to high throughput screening capacities, easy fluid handling and reduced costs related to device miniaturization, microfluidics has emerged as a powerful tool for single cell manipulation and analysis.
How to ask cells what proteins they produce?
The conventional way to isolate cells is by using Fluorescence Activation (FACS) to sort them into barcoded well plates (96 wells, 384 or 1536). This step is quite fast with the FACS currently available, but segregating thousands of cells into plates will result in a long process of pipetting. Droplet microfluidics allows for fast and low volume compartmentalization up to a thousand drops produced per second
Microfluidic Droplet Production Method
Droplet microfluidics is a powerful tool which consists of generating and manipulating micron sized monodispersed droplets. A Microfluidic based droplet has many diverse and varied applications such as particle synthesis and physicochemical analysis .A good control of droplet production can also make single-cell analysis, or drug testing possible. In this review we are presenting all microfluidic methods that can be used to produce droplets.