Agarose Microcapsules Synthesis
This application note describes the method for the production of double agarose emulsions using the RayDrop double emulsion chip and Fluigent pressure-based flow controllers. Agarose microcapsules are generated with precise control of particle, core and shell size.
Alginate Microbeads Production
Following is a method for encapsulation of reagent into alginate microbeads with total control of bead formation. A droplet-based microfluidic method is used to precisely control the production of microbeads without the drawbacks of large size distribution that present other methods.
Alginate Microcapsule Synthesis
In this application note, we describe the process of producing highly monodisperse alginate microparticles using droplet-based microfluidic methods to control droplet size and core-shell ratio. Different concentrations of alginate have been used to show the versatility and adaptability of the RayDrop in multiple applications.
Analysis of a commercial surfactant for digital PCR assay
In this application note we are investigating the usability of the commercially available surfactant dSurf for an exemplary digital PCR-assay.
Cancer Cell Analysis Made Easy with Aria: cell Capture and Labeling
We have demonstrated the use of Aria and its related software for the automated delivery of different liquids for the capture and labeling of cancer cells (breast cancer) using a complex microfluidic setup.
Capillary electrophoresis using microfluidic, electrophoretic, and optic modules
Here we present here the Lego CE system consisting of available ready-to-use electrophoretic and microfluidic modules, including pressure-based flow controllers. The instrument is coupled with a laser-induced fluorescence detector (LIF) and is demonstrated for separations of labeled oligosaccharides
Cartilage on chip using Fluigent MFCS pressure controller
Elucidating how chondrocytes react to external stimuli (mechanical or chemical) is important to understand processes triggering cartilage diseases like osteoarthritis. In this application note, we report on the use of Fluigent products to create complex mechanical stimulation patterns on 3D cell culture in a microfluidic platform, or so-called organ-on-a-chip device, with a specific focus on creating a cartilage-on-a-chip model.
Development of a human gut-on-chip to assess the effect of shear stress on intestinal functions
Our gut-on-chip system made of BEOnChip microfluidic chips coupled to pressure controllers offers a way to control and mimic the microenvironment of in vitro cultured intestinal epithelium that is closer to the physiological state.
Double Emulsion Generation
In this application note we have demonstrated how we can perform different type of double emulsion generation with a single device.
Droplet and particle manipulation using electrophoretic flow control
Many microfluidic applications are developed based on concepts relying on electrodes, exploiting the electrical properties of samples to sort or separate them, generate electro-osmotic flows, manipulate particles, perform electrochemical detection etc. One example of this is electronic paper, where the electrophoretic displacement of titanium dioxide particles in a dark hydrocarbon oil solution allows the formation of a black and white pattern with a high resolution.
Droplet Sequencing: Drop-Seq method
The Drop-Seq protocol, is a high throughput method that enables the sequencing of the mRNA from a large number of cells. With this method it is possible to create a gene expression map of the cell, or even distinguish cell populations within a tissue!
E. Coli Culture in Droplets Using dSURF Fluorosurfactant
In this application note, discover how using microfluidics allows one to encapsulate single or multiple cells into tiny droplets of pL volume which are generated at a rate of approximately one thousand per second.
Fluid recirculation for cell perfusion with reduced shear stress
Many microfluidic applications require expensive solutions to be injected at a controlled flow-rate into a microfluidic system, such as cell cultures, PCR processes, cell injections or simulation of blood capillaries with a controlled minimal mechanical stress.
Generation of bubbles using the RayDrop
Microfluidic devices are used for Bubble generation at micron-scale, yielding high monodispersity and well-controlled size. We investigated how parameters such as the geometry of the nozzle and the continuous-phase flow rate affects the microbubble formation process.
Generation of microcapsules with a UV-crosslinked polymer
Using the double emulsion Raydrop, controlled double emulsification is achieved by dripping or jetting the core fluid into an immiscible shell fluid, which is then encapsulated by the third fluid. In this note, capsules are formed by consolidating shell phase of the resulting double emulsions by uv-crosslinking of monomers and photoinitiator used as shell phase.
High-throughput cell DNA screening using digital PCR
Thanks to droplet microfluidic, Michael Ryckelynck and his team are able to isolate single DNA molecules and analyze the enzymes and proteins resulting from their expression.
How to Evaluate Cell Proliferation Using Pressure as a Tool
This application presents a simple method to monitor cell proliferation in microfluidic chips in real time. This is demonstrated experimentally using a custom microfluidic chip. Cell morphology was studied under flowing and static culture condition
Impedance Measurement of Microbeads
We present in this application note our Electrical Impedance Spectroscopy Platform (or EISP) consisting of microfluidic flow controllers from Fluigent to maintain precise flow control, a chip from Micronit Microtechnologies B. V to localize impedance measurements, and a lock-in amplifier from Zurich Instruments to perform impedance measurements. We demonstrate the system efficiency by determining the size of micrometer beads and by measuring the generation rate water-in-oil droplets.
Liposome Nanoparticle Synthesis
In this application note, the Raydrop (developed and manufactured by Secoya) is used to perform Liposome Nanoparticle synthesis.
Microbiome culture in droplet using dsurf surfactant
The performance of three commonly used surfactants are compared at three different concentrations. The droplet stability over time and the droplet occupation rate is determined by encapsulating a microbial community derived from human skin.
Microfluidic Chitosan Microcapsules Production
In this Application Note, chitosan-shell/oily-core microcapsules are generated using the Raydrop double emulsion chip, and Fluigent pressure-based flow controllers. The influence of the fluidic parameters on the size and the release from the oil across the shell are studied and presented.
Microfluidics for Transmission Electron Microscopy: Characterization of Copper Electrodeposition
This application presents the Liquid-phase Transmission Electron Microscopy (LPTEM) technique, which integrates integrates liquid flow capabilities within microfabricated liquid cells, providing the means to study different processes in solution with sub-nanometer spatial resolution and sub-microsecond temporal resolution. Using this technique, it is now possible to visually study topics ranging from material science to life science.
Multiple emulsion droplet generation
How to encapsulate multiple aqueous droplets (called “core”) into a single oil shell.
Oil in Water Emulsions
In this application note, we present droplet generation data obtained using decane in Water, a system that demonstrates the expected behavior of most hydrocarbons in Water. We demonstrate the ability of Fluigent equipment coupled with Raydrop microfluidic devices to generate high-quality emulsions with controlled droplet sizes and with high throughput.
Peristaltic Pump vs Pressure-Based Microfluidic Flow Control for Organ on Chip applications
The importance of flow stability in microfluidic systems is demonstrated by performing cell culture using peristaltic pumps or pressure-based flow controllers.
PLGA microcapsules synthesis
In this Application Note, PLGA shell/aqueous core microcapsules are obtained using the Secoya Raydrop Double emulsion. The influence of the fluidic parameters on the microcapsule size and release from the oil across the shell are explored in this application note.
PLGA Microparticles Synthesis
PLGA are of for life science applications. This application note focus on PLGA .
PLGA nanoparticle synthesis using 3D microfluidic hydrodynamic focusing
In this Application Note, PLGA nanoparticles with high monodispersity are generated using the Raydrop single emulsion developed and manufactured by Secoya, and Fluigent pressure-based flow controllers. The ability to synthesise PLGA nanoparticles in a more controllable and reproducible way creates possibilities to tailor surface properties and increase fields of application.
Pressure predictions for lab-on-a-chip operations using a microfluidic network solver and Fluigent PXDownloadDirect download
Production of water-in-oil emulsions using a droplet generator chip
In this application note, we demonstrate droplet generation using the Fluigent microfluidic system including pressure pumps, chemicals, tubing and a Droplet Generator Chip obtained from our partner microfluidic ChipShop.
Single cell sorter microfluidic platform
Passive cell or particle sorting using a dedicated package. To demonstrate the separation of particle mixtures, a solution containing 7.5 µm and 15 µm diameter polystyrene particles labeled with FITC, and TRITC fluorophores respectively was used. The particle streams were viewed and captured separately using appropriate filter cubes.
UV-Crosslinking of Microparticles
In this short note, we present a Fluigent microfluidic solution for polymer microparticle synthesis with high monodispersity (2% CV). The microfluidic system allows for inline particle generation, spacing, and polymerization by UV light.
Water in Fluorocarbon Oil Emulsions
In this application note, we present droplet generation data obtained using Fluigent’s own fluorocarbon oil formulation – dSURF to demonstrate the ability of Fluigent equipment coupled with Raydrop microfluidic devices to generate high-quality emulsions with controlled droplet sizes and with high throughput.
Water in Oil Emulsions
In this application note, we present droplet generation data obtained using one of the most widely used water in oil emulsion systems – water in decane. We demonstrate the ability of Fluigent equipment coupled with RayDrop microfluidic devices to generate high-quality emulsions with controlled droplet sizes and with high throughput.