This pack has been designed for high throughput experiments. The compactness and arrangement of the different elements have been optimized to perfuse a maximum number of chips in an incubator. It includes an 8 channel compact pressure controller, a flow rate platform, and reservoir holders directly adapted on an incubator trail.
Organ on a chip perfusion package - High Throughput[OOC-ST-001]
The perfect set for high throughput cell perfusion
- Designed for cell biology
- Easy to use
Designed for experiments parallelization
Cell seeding in 3D hydrogel is a delicate manipulation. Hydrogels are fragile and soft. They can easily fracture or detach from the chip if the flow is unstable.
Manual cell seeding is not recommended as the discontinuous flow results in heterogeneous cell seeding and can crack the hydrogel.
The manual mode of the Flow EZ is perfect to circumvent these limitations as it allows to implement small flow rate increments while keeping an eye on the microscope.
Stable & complex flow patterns
Fluigent’s products have unprecedented performances in terms of stability and responsiveness.
High stability is particularly useful for applications with constant shear stress like vascular perfusion.
High responsiveness is essential to reproduce complex flow patterns like aortic pressure variations. For complex flow pattern, the orders can be set in flow or pressure variations.
Once the parameters are set and optimized, protocol automation is the key to saving time, limiting contamination, and decreasing variability.
For any protocol, valves, pressure, and flow controller from Fluigent can be assembled to automate the protocol using user-friendly software (OxyGEN).
We can adapt the package to fulfill your needs of specific flow rates, or pressure
Cell culture under flow
Cultivating cells under flow perfusion has two main advantages: constant medium renewal and reproduction of mechanical strain in vitro.
To grow cells under perfusion, we recommend using the BE-Flow chip.
Cells are constantly exposed to biochemical stimulation from the early embryonic stage to adult life. The spatiotemporal regulation of these signals is essential as it determines cell fate, phenotype, metabolic activity as well as pathological behaviors. Biochemical stimulation is also central in oncology as it drives the migration and expansion of malign tumors.
To study chemotaxis in 3D hydrogel we recommend using BE-GRADIENT.
Co-culture under perfusion
Co-culture under perfusion is essential for various applications:
- to fully differentiate given cell types, coculture with another cell type is required
- in cosmetics or drug testing to reproduce interfaces link skin, lung or gut and monitor the uptake of active molecules
- to reproduce liquid-liquid or air-liquid interfaces.
The BE-Doubleflow chip best fits these applications as a porous membrane separates the central chamber into two channels.
Transwell under flow
Transwells are classic devices widely used in standard cell culture to co-culture cell types, reproduce 2D-2D interfaces, 3D-2D interfaces, study cell invasion, assess monolayer permeability or active molecule uptake.
For this application, we recommend the BE-Transflow
|Fluigent’s MFCS-EX (up to 8 channels)|
|Fluigent’s Flow Unit (up to 8 flow unit packed in one platform)|
|Incubator grid (On demand custom grid with reservoir holders)|
|BEOnChip microfluidic chip|
|Fluigent connectors and tubing kit|
|Control in real-time, protocol automation, data record and export|
|ver. 188.8.131.52 or more recent|
Software Development Kit
|Custom software application|
|ver. 184.108.40.206 or more recent|
Connect the chip to the microfluidic system
Switching from static culture to fluid perfusion