< 2% CV
Nanoparticle Production Pack
[O-MIX-LIPO-PCK] or [O-MIX-PLGANP-PCK]Complete system for producing nanoparticles in a reproducible manner
A nanoparticle is defined as a particle with dimensions below the micrometer range. Typically, their diameter ranges between a few dozen and a few hundred nanometers. In particular, liposomes are nano-vesicles of spherical shape with a lipid bilayer. They are commonly used as carriers for active pharmaceuticals ingredients.
The Fluigent Nanoparticles Production Pack is a robust and complete system for precise and long-term production of nanoparticles, including liposomes, with high flexibility in the particle size range. The pack includes Fluigent’s LineUP microfluidic pumps and Secoya's RayDrop device – a breakthrough in high-quality particle production technology.
Main benefits
High precision- Flexible
Adjustable particle size
- Robust
Continuous production
Features of the Pack
Complete System
With this nanoparticle production pack, users have all the components needed to start generating nanoparticles with high monodispersity and high stability.
Dedicated Protocol
A protocol is available to assist users in setting up and starting their experiments.
Engineered Solution
We built the nanoparticle station with the right pressure controllers, microfluidic chips, and valves to give you the greatest possible flexibility in terms of particle size and generation rate.
Customization
We can adapt the package to meet your needs (particle size, generation rate).
Related applications
Why use microfluidics for nanoparticle synthesis?
Conventional methods for nanoparticle production rely on bulk protocols such as precipitation, chemical reduction, microemulsions or seeding. While these techniques are easy to handle, an overall lack of control over the particle size and poor batch-to-batch reproducibility make them unsuitable for biomedical applications such as drug development or diagnostics. Microfluidics, the method used in this nanoparticle station, appeared in the 2010s as a powerful tool to overcome these limitations.
Its ability to generate control fluid flows and chemical exchanges in a reproducible manner allows users to produce large quantities of nanoparticles with a precise diameter, excellent monodispersity, and high reproducibility.
Main Products of the Nanoparticle station
With the components of the nanoparticle production pack, users can produce nanoparticles and liposomes with high monodispersity. This pack controls the size of the nanoparticles, increasing the range of applications in which they can be used.
Specifications
| LineUp Flow EZ pressure controller (2000 mbar) x2 |
| LineUp LINK Module (software control) x1 |
| FLOW UNIT M (x1) and L (x1) |
| P-CAP series 15 mL (x3) |
| LineUp Switch EZ (x1) |
| 2-SWITCH x2 |
| Raydrop Single Emulsions x1 |
| SWITCH EZ x1 |
Liposome Nanoparticles Production
| Inner phase | Absolute ethanol + Lipids 10 mg/mL + Dimethyldioctadecylammonium (DDAB) 2.5 mg/mL |
| Outer phase | Phosphate-buffered saline (PBS) (pH 7.2) |
| Particle size range | 30 nm to 150 nm |
| Polydispersity | From 7% to 15% |
PLGA Nanoparticles Production
| Inner phase | Acetone + Poly(lactic-co-glycolic acid) (PLGA) Resomer 756 1%hanol + Lipids 10 mg/mL + Dimethyldioctadecylammonium (DDAB) 2.5 mg/mL |
| Outer phase | Deionized water + Polyvinyl alcohol (PLGA) Mw 90000 – 10000 80 % hydrolyzed |
| Particle size range | 100 to 250 nm |
| Polydispersity | From 5% to 15% |
Flow Control
| Pumps | Fluigent Flow EZ (2000mbar) |
| Flow Sensors | Fluigent FLOW UNIT (M & L) |
| Automated valves | Fluigent 2-SWITCH |
Imaging (optional)
| Microscope | Fluigent Digital high-speed microscope |
OxyGEN
| Control in real-time, protocol automation, data record and export |
| ver. 2.2.0.0 or more recent |
Software Development Kit
| Custom software application |
| ver. 22.2.0.0 or more recent |
Imaging
| Pixelink Capture Software |
| Extrusion method | Fluigent microfluidic method | |
| Particle size distribution | up to 50% | ~ 2% |
| Reproducibility | low | High |
| Particle size control | no | Precise |
| Continuous (in line) production | no | yes |