Reliable droplet formation and high purity
Highly stable fluorosurfactant for microdroplet generation[DR-RE-SU]
dSURF is a highly stable fluorosurfactantsuitable for the production of highly monodispersed and stable microdroplets. Provided at 2% in 3M™ Novec™ 7500 fluorinated oil, dSURF allows high-performance droplet formation and long-term stability even in challenging conditions such as dPCR and cell culture experiments.
- High monodispersity
Compatible with long-term cell culture experiments
FAM™, HEX™, VIC® and EvaGreen® based dPCR validated
Importance of a Highly Stable Fluorosurfactant for Droplet Generation in Microfluidics Experiments
A key parameter in droplet microfluidics experiments involves finding the best surfactant for stable and reproducible droplet generation.
What is a surfactant?
A surfactant is a molecule composed of a hydrophilic head and a hydrophobic tail, positioned at the interface between the aqueous droplet and the continuous oil solution. It lowers the interfacial tension, enabling longer droplet stability and preventing droplet merging. Using a highly stable fluorosurfactant like dSurf is critical to ensuring good stability for your emulsion.
Unique Properties of Fluorinated Surfactants for Droplet Generation
What are Fluorinated Surfactants used for?
Fluorinated surfactants are often used for droplet generation because they have a number of unique properties that make them ideal for this purpose. These properties include:
- High stability: Fluorinated surfactants can form very stable monolayers at the air-water interface, which can help produce droplets with a narrow size distribution and high stability.
- Low surface tension: Fluorinated surfactants typically have low surface tension, so they can help reduce the interfacial tension between the droplet and the surrounding fluid and make it easier to generate droplets of a specific size.
- Resistance to degradation: Many fluorinated surfactants are resistant to degradation, which means that they can maintain their properties over time, even in harsh environments.
- Chemical inertness: Fluorinated surfactants are often chemically inert, which means that they do not react with other chemicals or substances, making them ideal for use in a wide range of applications.
“dSURF improved our droplet stability and reliability of droplet formation under control of Fluigent systems. We performed good quality dPCR and droplet-based micro-cultivation of microbial.”
Dr. Thomas Henkel – Head of Microfluidic group – Leibniz IPHT, Germany
„The dSURF is super good! It proved compatibility with the culture of mammalian cells, with no visible toxicity as compared to control.“
Ya ZHOU – Postdoctoral Research Fellow – Macromolecules and Microsystems in Biology and Medicine Laboratory Institut Curie, Paris
Features of the dSurf
Seamless downstream application compatibility
dSURF makes no compromise on quality. Its unique formulation ensures reliable results for any application. From dPCR and single-cell analysis to cell culture, the biocompatibility of Fluigent’s highly stable fluorosurfactant has been validated even in harsh/extreme conditions. The dOIL fluorinated oil is also PDMS compatible, as its low viscosity enables the use of lower pressures to achieve optimum flow rates.
When using droplets for cell analysis, both sample cross-talk and dispersion should be minimized for greater accuracy and minimal reagent loss. dOIL (Novec™ fluorinated oil) offers a low drop-to-drop transfer of organic compounds while maintaining high gas solubility and permeability, which have a major impact on cell survival and analytical reaction efficiency.
Batch to batch reproducibility
Droplet generation reproducibility and stability are two critically important parameters for successful applications. Fluigent guarantees the best conditions for reliable results by providing a high-quality surfactant.