Fluid recirculation for cell perfusion with reduced shear stress
Many microfluidic applications require expensive solutions to be injected at a controlled flow-rate into a microfluidic system, such as cell cultures, PCR processes, cell injections or simulation of blood capillaries with a controlled minimal mechanical stress.
In this application, two MFCS™-EZ pressure channels are connected to two vials containing a physiological buffer for instance. The use of the L-SWITCH™ and ESS™ Control software allows for recirculation between the two vials while maintaining a continuous unidirectional flow-rate within the cell culture chamber.
Benefits
- Small buffer volume
- Controlled shear stress
- Long-term experiments
- High stability
- Flexible automation
- Pressure and/or flow-rate control and limit
Materials needed
- 1 MFCS™-EZ
- 1 L-SWITCH™
- 1 FLOW UNIT
- Fluigent software
