Fast valve actuation time (< 100 ms)
Sample injection and recirculation microfluidic valve for industry
[IVLSW1]Fluigent L-X
The L-X is a bidirectional 6-port/2 position microfluidic valve. It is designed for precise sample injection or fluid recirculation in cell culture applications.
Main benefits
Quick- Accurate
Low internal volume
- Compact
Designed for system integration
- Biocompatible
Chemically and biologically compatible
Features
Precise fluid injection
Several sample loops are available, from 5 µL to 100 µL.
A versatile valve
The valve can be operated using pressure up to 7 bar (100 psi), and the wetted material is PEEK, a highly chemically and biocompatible material.
Fluigent software for automation
The valve can be controlled by Fluigent software for long-term experiments. Create a time-based protocol to set actuation timing of the valve(s).
Minimized liquid waste
Low port-to-port volume of 660 nL
Related applications
Working principle
Peripheral ports (numbered from 1 to 6) can alternatively be connected the right or the left channels. The L-X is actuated by a motor that drives a rotor – where the fluidic path is engraved – against a stator – containing the fluidic paths.
Fluid recirculation
The L-X can be used as a useful cell culture tool: a small volume of buffer can be recirculated within a closed loop into the chip for several hours or days. Combined with our pressure-based flow controllers, it can achieve a highly stable flow rate, allowing to better mimic flow-induced shear stress experienced by cells in vivo.
Precise fluid injection
The L-X can be used as a useful cell culture tool: a small volume of buffer can be recirculated within a closed loop into the chip for several hours or days. Combined with our pressure-based flow controllers, it can achieve a highly stable flow rate, allowing to better mimic flow-induced shear stress experienced by cells in vivo.
Specifications
PERFORMANCE
| Pressure rating | Up to 7 bar (100 psi) |
| Stability | 0,1% on the measured value (effective beyond 10% of the maximum pressure) |
| Response time | 100 ms |
| Step | 0,03% of the maximum pressure |
HARDWARE SPECIFICATIONS
| Dimensions | 70 x 90 x 150 mm |
| Weight | 475 g |
| Internal volume | 660 nL |
ELECTRONICAL SPECIFICATIONS
| Power supply | RJ45 |
CHEMICAL COMPATIBILITY
| Gas compatibility | Dry, oil-free gas, air, any non corrosive or non explosive gas |
| Liquid compatibility | Aqueous solvent, oil, organic solent, biological sample |
| wetted materials | PEEK |
Fluigent pressure-based flow controllers VS Peristaltic pump
| Fluigent pressure-based flow controllers | Peristaltic pumps | |
| Flow stability | High (<1% variation) | Low (>20% variation) |
| Response time | High | Medium |
| Pressure control | Yes | No |
| Price | Medium-High | Low |
| Implementation | Requires pressure sources | Easy |
OxyGEN
| Control in real-time, protocol automation, data record and export |
| ver. 1.0.0.0 or more recent |
Software Development Kit
| Custom software application |
| ver. 21.0.0.0 or more recent |
How to add the L-X in my experiment and how to connect it?
- Connect the L-X to the SWITCH EZ port
- Cut the 1/16’’ OD tubing to the desired length, leaving a square-cut face.
- Insert the tubing into a nut until it passes 1.5 – 3mm.
- Insert into a port of L-X, twist until it is tightened. You can pull gently the tubing to verify that it is securely connected to the port.
1. HOW TO CONNECT A SAMPLE LOOP TO THE L-SWITCH™?
How to connect a sample loop to the L-X?
- In order to connect a sample loop to the L-SWITCH™, you will need two nuts and two ferrules, in addition to the sample loop itself (nuts and ferrules are provided with the sample loop).
- Pass one end of the sample loop through a nut.
- Add a ferrule
- Hold the set together and insert it into a port of the L-SWITCH™ (for example, port 1 here).
- Twist until the set is tightened. You can pull gently the sample loop to verify that it is securely connected.
- Repeat these steps for the other end of the sample loop to connect it to another port of the L-SWITCH™ (for example port 4 here).
How can I clean the L-X after use?
You can clean the L-Switch™ in the same way than the Flow Unit.
Besides, Fluigent strongly advises you to use filtered solutions. Wetted materials are PEEK only.