Designed for system integration
Sample injection and recirculation microfluidic valve for industry[IVLSW1]
The L-X OEM 6 port 2 position valve is a bidirectional microfluidic valve designed for precise sample injection or fluid recirculation in cell culture applications.
Actuation and response time
Low internal volume
Fully automated with in house software or SDK libraries
|Aqueous solvent, oil, organic solent, biological sample
Sample injection and recirculation microfluidic valve for industry
Fluigent L-X valve features
Precise fluid injection
Several sample loops are available, from 5 µL to 100 µL.
Fluigent software for automation
The L-X microfluidic valve can be controlled by Fluigent software for long-term experiments. Create a time-based protocol to set actuation timing of the valve(s).
A versatile valve
The OEM 6 port 2 position valve can be operated using pressure up to 7 bar (100 psi), and the wetted material is PEEK, a highly chemically and biocompatible material.
Minimized liquid waste
Low port-to-port volume of 660 nL.
Fluigent pressure-based flow controllers VS Peristaltic pump
|Fluigent pressure-based flow controllers
|High (<1% variation)
|Low (>20% variation)
|Requires pressure sources
How to use the L-X device ?
Peripheral valve ports (numbered from 1 to 6) can alternatively be connected to the right or the left channels. The L-X OEM microfluidic valve is actuated by a motor that drives a rotor – where the fluidic path is engraved – against a stator – containing the fluidic paths.
The L-X 6 port 2 position valve can be used as a useful cell culture tool: a small volume of buffer can be recirculated within a closed loop into the chip for several hours or days. Combined with our pressure-based flow controllers, it can achieve a highly stable flow rate, allowing to better mimic flow-induced shear stress experienced by cells in vivo.
The figure shows the working principle: fluid is injected from reservoir 1 to reservoir 2 and passes through the L-X. When reservoir 1 is almost empty, fluid is injected from reservoir 2 to reservoir 1, and the port positions of the L-X are switched so that fluid is injected on the same direction, which is usually required for cell culture under flow.
How to add the L-X in my experiment and how to connect it?
- Connect the L-X to the SWITCH EZ port
- Cut the 1/16’’ OD tubing to the desired length, leaving a square-cut face.
- Insert the tubing into a nut until it passes 1.5 – 3mm.
- Insert into a port of L-X, twist until it is tightened. You can pull gently the tubing to verify that it is securely connected to the port.
How to connect a sample loop to the L-SWITCHTM?
How to connect a sample loop to the L-X?
- In order to connect a sample loop to the L-SWITCH™, you will need two nuts and two ferrules, in addition to the sample loop itself (nuts and ferrules are provided with the sample loop).
- Pass one end of the sample loop through a nut.
- Add a ferrule
- Hold the set together and insert it into a port of the L-SWITCH™ (for example, port 1 here).
- Twist until the set is tightened. You can pull gently the sample loop to verify that it is securely connected.
- Repeat these steps for the other end of the sample loop to connect it to another port of the L-SWITCH™ (for example port 4 here).
How can I clean the L-X after use?
You can clean the L-Switch™ in the same way than the Flow Unit.
Besides, Fluigent strongly advises you to use filtered solutions. Wetted materials are PEEK only.
|Control in real-time, protocol automation, data record and export
|ver. 126.96.36.199 or more recent
Software Development Kit
|Custom software application
|ver. 188.8.131.52 or more recent