Digital PCR (dPCR)
The science of microfluidic flow control for droplet digital PCR (ddPCR)
- Higher accuracy
- Higher sensitivity
- Absolute quantitation
Liquid biopsies are non-invasive tests performed on a blood sample to detect cancer cells circulating in the blood (circulating tumor cells, CTCs) or pieces of DNA from tumor cells in the blood. This technique is increasingly used for cancer detection and monitoring, as they are low risk for the patient and help doctors understand what kind of molecular changes are taking place in the tumor
Copy number variation
A key measurement challenge in diagnostic research involves identifying small changes in nucleic acid sequence that are commonly associated with genetic diseases76. Changes in the genomic DNA leading to an abnormal copy of a DNA sequence are called copy number variations (CNVs). They are involved in many complex diseases such as Down’s syndrome, and many cancers.
Pathogen Detection and Microbiome Analysis
Microbiology is one of the fields where digital PCR is extensively used. In fact, detection of low-level pathogens is important in situations where viruses or bacteria can cause human diseases. As digital PCR shows higher sensitivity compared to standard PCR, and the ability to amplify low concentration targets even in complex backgrounds, it is a technology of choice for microbiome analysis.
Importance of fluid handling for droplet digital PCR application
Droplet digital PCR relies on random distribution of dPCR mix containing target molecules on the partition of equivalent volumes (here, the droplets). In this way, some partitions contain no target molecules while the remaining partitions contain at least one molecule. Partitions are next categorized and counted as positive or negative depending on their fluorescence intensity. The calculations are derived from a Poisson model, which can be impacted by partition volume since the model assumes the partition to be monodisperse. As a consequence, a heterogeneous droplet population can affect the dPCR process. In fact, several groups demonstrated through different studies that partition droplet volume variability can cause a bias on the accuracy of the measurements.
More information can be found on the paper written by Emslie et al.: Droplet Volume Variability and Impact on Digital PCR Copy Number Concentration Measurements The impact of flow rate on droplet size using a microfluidic system is today well described in the literature. Thus, to avoid heterogeneous droplet populations, one should consider using a precise flow controller.
Flow control systems for digital PCR
Flow rate stability is thus critical for having repeatable reactor volumes and reproducible results. Syringe pumps are commonly used for generating droplets. Depending on the model in use, syringe pumps show limited flow control. As a consequence, the droplet size, proportional to the flow rate, is affected. In addition, the actual flow rate cannot be monitored with such devices. The flow rate value is displayed on the device, but no information on the time required for reaching a set flow rate is given (the time for flow equilibrium may vary depending on the microfluidic setup, and flow can oscillate depending on the instrument). An alternative to syringe pumps is pressure-based flow controllers. These show high-precision flow control, fast reaction time, and flow monitoring are possible.
We compared the production of water-in-oil emulsions using microfluidic syringe pumps and pressure-based flow controllers. Using pressure control, the desired droplet size is quickly obtained (< 6 s), and monodisperse droplet generation is ensured over time
The benefits of choosing Fluigent for your flow droplet digital PCR system
- Best in class stability: < 0.5% thanks to our field-proven, patented FASTAB™ technology allowing optimal flow control with the robustness required in demanding industrial environments.
- Workflow automation becomes straightforward thanks to the included Fluigent software
- Our engineering team are experts in microfluidic design, mechanical and software integration in addition to biology and application knowledge, which makes up a very versatile and capable team
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