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BeonChip – BE-DOUBLEFLOW STANDARD

Explore the crosstalk between different 2D and 3D cultures

BE-Doubleflow is Beonchip’s most advanced device. It consists of two perfusable channels connected via a porous membrane. Explore the crosstalk between different 2D and 3D cultures in a biomimetic environment and control the efficiency of the interaction by selecting the optimal pore size for your application. This is the optimal device when a hypoxia environment is needed, for studying the effect of circulating particles (bacteria, immune system, circulating tumor cells) and for endothelium/epithelium barrier when no ALI is needed or when flux plays a role in both sides of the coculture.

BE-DOUBLEFLOW STANDARD

Culture Models

BE-DOUBLEFLOW STANDARD

Explore the crosstalk in the endothelium epithelium barrier under flow in both channels of the device, mimicking a more physiological environment for the study of different organs such as kidney, liver, gut or heart, among others.

BE-DOUBLEFLOW STANDARD
BE-DOUBLEFLOW STANDARD

Combine 3D and 2D cultures in a hypoxia environment where oxygen is supplied to the cells via the medium flowing in one of the channels.

BE-DOUBLEFLOW STANDARD
BE-DOUBLEFLOW STANDARD

Research the effect of circulating particles such as circulating tumor cells, immune system cells, bacteria, fungi, viruses and many more in a single cell culture, or in a complex coculture.

How to video

Device features

  • Easy to use: Be-Doubleflow is compatible with any type of optical microscopy and its slide format has been chosen for easy handling under a microscope.
  • Easy to connect: Be-Doubleflow is compatible with all microfluidic flow control systems (syringe, peristaltic pumps, pressure control systems, rocker systems…).
  • No unspecific absorption: Unlike in other PDMS devices, Be -Doubleflow is made of lipophobic thermoplastic materials and does not present unspecific drug absorption issues. It allows inmunohistochemistry with fluorescent detection.
  • Cell recovery: The cell cultures used in the Be-Doubleflow can be easily recover for further experimentation.

Applications

BE-Doubleflow allows endothelium/epithelium barrier coculture where a hypoxia environment is needed or when flux plays a role also in the epithelium culture (Kidney, liver, heart, lung, gut…). In addition, its two perfusable channels offer a perfect environment for studying the effect of circulating particles (bacteria, immune response, circulating tumor cells).

Example applications

Kidney on chip: The coculture of HPTC cells and vascular endothelial cells clearly improves performance of the HPTCs and increases the maintenance of the renal epithelia in vitro. Using BE-Doubleflow it is possible to coculture both type of cells simultaneously, circulating urine on the renal epithelium culture and blood like medium in the vascular culture.

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