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Immunostaining Pack
O-MULTILABEL-PCKA ready-to-use package for sequential injection of multiple fluids for cell or tissue staining
The Immunostaining Pack provides stable and automated delivery of up to 10 different fluids to a single cell culture chamber.
Main benefits
Easy- Designed for cell biology
Contamination-free, Sterile environment
- Compact
Inject up to 10 different samples
- Versatility
Possibility to heat or agitate reservoir
Why use sequential injection for immunostaining?
Immunostaining is a multi-step technique for detecting the presence or localization of specific proteins, molecules, or cells within a biological or tissue sample.
Various reagents such as fixating agents or permeabilization buffers, are sequentially added to the samplewith incubation steps between each injection.
In the classic approach, all the reagents are added by manual pipetting: a time-consuming process that greatly increases the likelihood of errors occurings. Using microfluidic systems overcomes the limitations of conventional protocols. Automating the sequential steps by using our pressure-based flow controllers coupled with rapid low-volume valves not only saves time for the biologist, but also greatly improves the reproducibility of the assay. Switching from a bulk assay to a microfluidic format also allows for reduced reagent and sample volumes and shorter incubation times.
Our Immunostaining Pack combines all the equipment, software, and information you need to easily transfer your experiment from a bulk protocol to a microfluidic assay.
Features of the sequential injection pack
Complete system
All the components you need for your experiment are included in the Immunostaining Pack.
Versatility
This package can be used for diverse applications, and with any microfluidic chip.
The reservoirs can be easily manipulated for heating, agitation, etc.
Stable flow rate & short switching time
Fluigent’s products offer very stable delivery and short switching times to ensure optimal results for any immunostaining experiment.
Large sample volumes
Ten injection reservoirs allow for easy manipulation of large sample volumes, and our pressure-based flow controller offers a wide range of pressure and flow rates.
Dedicated protocol for sequential injections
Up to 10 selected liquids can be delivered sequentially to a chip using the M-SWITCH. A 2-SWITCH can also be used to sort the samples at the outlet of the chip to a collection tube or to waste.
Build customized and time-based protocols to save time and reduce variability with our user-friendly OxyGEN software, designed for easy and complete protocol automation.
Related applications
- Immunostaining
- Chip preparation : Chip coating, cell loading, cell incubation
Main components of the Immunostaining Pack
Pack Specifications
PRODUCTS
Our Immunostaining Pack includes the following components:
| LineUp Flow EZ pressure controller (1000 mbar) (x1) |
| LineUp LINK Module (software control) (x1) |
| FLOW UNIT M (x1) |
| 2-SWITCH (x1) |
| SWITCH EZ (x1) |
| P-CAP series 15 mL (x12) |
| M-SWITCH (x1) |
| 10 Position Pressure Manifold (x1) |
FLUID HANDLING SYSTEM
| Product | Part number |
| 1*Flow EZ | LU-FEZ-1000 |
| 1*2-SWITCH | 2SW002 |
| 1*SWITCH EZ | ELUSEZ |
| 1*LINK module | LU-LNK-0002 |
| 1*M-SWITCH | ESSMSW003 |
RESERVOIRS
| Product | Part number |
| 12*15 mL Pcap with 15 mL Falcon tube | P-CAP15-HP |
| 1*10 Position Pressure Manifold | CTQ-MANI |
FLOW METERS
| Product | Part number |
| 1*Flow unit M | FLU-M-D |
TUBING
| Product | Part number |
| Tubing and connection Kit P-CAP 15mL FEP tubing with an ID of 500 microns | CTQ-KIT-PC15 |
| 1*Tubing and connection Kit 2-SWITCH | CTQ-KIT-2SW2 |
| 1*Tubing and connection Kit Flow UNIT M | CTQ-KIT-LQ |
| 1*Tubing and connection Kit M-SWITCH | CTQ-KIT-ESSMSW003 |
OxyGEN
| Real-time control, automated protocol, data record and export |
| ver. 2.2.0.0 or more recent |
Software Development Kit
| Custom software application |
| ver. 22.2.0.0 or more recent |
“We have been using Fluigent’s M-SWITCH, amongst other accessories, including Flow EZ system, flow controllers, for the past 5 years. We are pleased with its performance, especially the programmability and automation of fluid flow that relieves the user of the need to be present next to the experiments that run for several hours. We found the software interface to be simple to use and could count on the team’s assistance for friendly recommendations and support.”
Sivashankar Krishnamoorthy – Luxembourg Institute of Science and Technology (LIST)
Aria: a fully integrated system
Run sequential perfusion and immunostaining experiments in an automated, intuitive, and completely integrated system with our ready-to-use Aria system.
The Aria is our automated solution for cellular perfusion or timed injection protocols, allowing for the automatic delivery of up to 10 different solutions into a chamber or microfluidic chip, according to user-defined protocols.
