Simple connections
Immunostaining Package
O-MULTILABEL-PCKA ready-to-use package for sequential injection of multiple fluids for cell or tissue staining
The Immunostaining Package provides stable and automated delivery of up to 10 different fluids to a single cell culture chamber.
Main benefits
Easy- Designed for cell biology
Contamination-free, Sterile environment
- Compact
Inject up to 10 different samples
- Versatility
Possibility to heat or agitate reservoir
Features
Complete system
All components necessary for immunostaining included in the package.
Versatility
This package can be used for diverse applications, and with any microfluidic chip.
The reservoirs can be easily manipulated, to be heated or agitated for example.
Stable flow rate & short switching time
Fluigent’s products allow for a very stable delivery and a short switching time for the best results in immunostaining.
Dedicated protocol
Build customized, time-based protocols to save time and reduce variability with our user-friendly software OxyGEN.
Our software allows easy and complete protocol automation.
Large volume sample
Ten injection reservoirs allow for the manipulation of large volumes of samples.
A large range of pressure and flow rates are available thanks to our pressure based flow controller.
Sequential injections
Up to 10 sequentially selected liquids be delivered to a chip by the M-SWITCH. The samples at the outlet of the chip may also be sorted using a 2-SWITCH either into a collection tube or to waste.
User-friendly software (OxyGEN) is available for each step.
Related applications
- Immunostaining
- Chip preparation : Chip coating, cell loading, cell incubation
Main products of the Immunostaining package
Specifications
PRODUCTS
Our Immunostaining Package contains the following :
| LineUp Flow EZ pressure controller (1000 mbar) (x1) |
| LineUp LINK Module (software control) (x1) |
| FLOW UNIT M (x1) |
| 2-SWITCH (x1) |
| SWITCH EZ (x1) |
| P-CAP series 15 mL (x12) |
| M-SWITCH (x1) |
| 10 Position Pressure Manifold (x1) |
FLUID HANDLING SYSTEM
| Product | Part number |
| 1*Flow EZ | LU-FEZ-1000 |
| 1*2-SWITCH | 2SW002 |
| 1*SWITCH EZ | ELUSEZ |
| 1*LINK module | LU-LNK-0002 |
| 1*M-SWITCH | ESSMSW003 |
RESERVOIRS
| Product | Part number |
| 12*15 mL Pcap with 15 mL Falcon tube | P-CAP15-HP |
| 1*10 Position Pressure Manifold | CTQ-MANI |
FLOW METERS
| Product | Part number |
| 1*Flow unit M | FLU-M-D |
TUBING
| Product | Part number |
| Tubing and connection Kit P-CAP 15mL FEP tubing with an ID of 500 microns | CTQ-KIT-PC15 |
| 1*Tubing and connection Kit 2-SWITCH | CTQ-KIT-2SW2 |
| 1*Tubing and connection Kit Flow UNIT M | CTQ-KIT-LQ |
| 1*Tubing and connection Kit M-SWITCH | CTQ-KIT-ESSMSW003 |
OxyGEN
| Real-time control, automated protocol, data record and export |
| ver. 2.1.0.0 or more recent |
Software Development Kit
| Custom software application |
| ver. 21.0.0.0 or more recent |
“We have been using Fluigent’s M-SWITCH, amongst other accessories, including Flow EZ system, flow controllers, for the past 5 years. We are pleased with its performance, especially the programmability and automation of fluid flow that relieves the user of the need to be present next to the experiments that run for several hours. We found the software interface to be simple to use and could count on the team’s assistance for friendly recommendations and support.”
Sivashankar Krishnamoorthy – Luxembourg Institute of Science and Technology (LIST)
Expertise & resources
For more integration
For a completely integrated, ready-to-use, and intuitive system to perform sequential perfusion and immunostaining experiments, Aria is the designated product.
Aria is our automated solution for cellular perfusion or timed injection protocols. Aria allows one to automate delivery up to 10 different solutions into a chamber or microfluidic chip by following user defined protocols.
