High throughput single cell counting in droplet-based microfluidics
By Heng Lu, Ouriel Caen, Jeremy Vrignon, Eleonora Zonta, Zakaria El Harrak, Philippe Nizard, Jean-Christophe Baret and Valérie Taly, from the Université Paris Sorbonne Cité and the CRPP at Bordeaux, France.
“Droplet-based microfluidics is extensively and increasingly used for high-throughput single-cell studies. However, the accuracy of the cell counting method directly impacts the robustness of such studies. We describe here a simple and precise method to accurately count a large number of adherent and non-adherent human cells as well as bacteria. Our microfluidic hemocytometer provides statistically relevant data on large populations of cells at a high-throughput, used to characterize cell encapsulation and cell viability during incubation in droplets.”
These experiments were made possible with the use our pressure-driven flow controller pump and it is a honor to be cited also at the end with a nice note: “We would like to thank Fluigent for collaborating with us on the cell encapsulation solutions”.
For more information: doi:10.1038/s41598-017-01454-4 or Scientific Report 7, 2017, 1366