The Fluigent/Micronit organ-on-a-chip platform focuses on modeling the main biochemical and biophysical features of the native environment of cells in order to induce their growth and differentiation as functional tissues. Air-liquid interface, flow induced shear stress, mechanical stimulation, biochemical gradient, cell-cell coculture have been reported to significantly improve the functionality of in vitromodels. All these parameters are controlled by the system.
Background image (repeat): Skin on chip grown on the membrane. Courtesy of Dr A El Ghalbzouri, LUMC, The Netherlands.
Fluigent has partnered with Micronit to develop a versatile fully integrated organ on a chip platform which reproduces numerous characteristics of the in vivo environment of cells. This platform comprises a flow control system connected to a resealable glass chip separated into two flow chambers by a transversal porous membrane.
Trans-membrane invasion assay
Quantification of molecular uptake and transport
Tissue/ cell stretching
Cell culture under perfusion
Hyperoxia /hypoxia in cell culture
Long term stable cell culture under perfusion (up to 1 month)
Air/liquid or liquid/liquid interfaces with distinct monitored flow rates in each chamber
Reproduction of flow induced physiological shear stress
Cell co-culture in controlled microenvironments
Biomechanical stimulation through membrane stretching
Sample collection from apical and basolateral flow chambers
Micropipette aspiration is a powerful non-invasive technique to evaluate how biomechanical properties of single cells or tissue govern cell shape, cell response to mechanic stimuli, transition from nontumorigenic to tumorigenic state or morphogenesis. The Fluigent MFCS™-EZ and Flow EZ™ pressure controllers are particularly suited for this method since it requires applying forces ranging from 10pN […]
Many microfluidic applications require expensive solutions to be injected at a controlled flow-rate into a microfluidic system, such as cell cultures, PCR processes, cell injections or simulation of blood capillaries with a controlled minimal mechanical stress. FIND A PUBLICATION DOWNLOAD FLYER DOWNLOAD FLYER Your Name*Your Email* I accept that by hitting submit I will be […]
Individual cell heterogeneity within a population has invalidated historic classification methods based on macroscopic considerations and given rise to new evaluation techniques based on single cell transcriptional signature. In this context, thanks to high throughput screening capacities, easy fluid handling and reduced costs related to device miniaturization, microfluidics has emerged as a powerful tool for […]