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Organ-on-a-chip-platform

The Fluigent/Micronit organ-on-a-chip platform focuses on modeling the main biochemical and biophysical features of the native environment of cells in order to induce their growth and differentiation as functional tissues. Air-liquid interface, flow induced shear stress, mechanical stimulation, biochemical gradient, cell-cell coculture have been reported to significantly improve the functionality of in vitromodels. All these parameters are controlled by the system.

Background image (repeat): Skin on chip grown on the membrane. Courtesy of Dr A El Ghalbzouri, LUMC, The Netherlands.

Fluigent has partnered with Micronit to develop a versatile fully integrated organ on a chip platform which reproduces numerous characteristics of the in vivo environment of cells. This platform comprises a flow control system connected to a resealable glass chip separated into two flow chambers by a transversal porous membrane.

organ-on-a-chip-platform-fluigent-set-up

Applications

Microphysiological systems/Organ-on-chip

Trans-membrane invasion assay

Quantification of molecular uptake and transport

Drug discovery/screening

Tissue/ cell stretching

Cell culture under perfusion

Hyperoxia /hypoxia in cell culture

Benefits

Long term stable cell culture under perfusion (up to 1 month)

Air/liquid or liquid/liquid interfaces with distinct monitored flow rates in each chamber

Reproduction of flow induced physiological shear stress

Cell co-culture in controlled microenvironments

Biomechanical stimulation through membrane stretching

Biochemical gradient

Sample collection from apical and basolateral flow chambers

organ-on-chip-hut-on-chip-intestinal-epithetial-cells

Osmo mechanical compression of a tissue confined in a microfluidic channel (Two photon imaging of intercellular space). Photo courtesy of Dr Sylvain Monnier (Institut Lumière et Matière, France)

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