Droplet and particle manipulation using electrophoretic flow control
Many microfluidic applications are developed based on concepts relying on electrodes, exploiting the electrical properties of samples to sort or separate them, generate electro-osmotic flows, manipulate particles, perform electrochemical detection etc. One example of this is electronic paper, where the electrophoretic displacement of titanium dioxide particles in a dark hydrocarbon oil solution allows the formation of a black and white pattern with a high resolution.
Most of the time, this implies complex microfabrication processes to build embedded electrodes inside the microfluidic chip. This can be avoided by the use of Fluigent’s Electrowell device, which combines pressurized fluid reservoirs with a choice of electrodes. These charged reservoirs can be simultaneously connected to an MFCS™-EZ pressure controller and a high voltage generator in order to generate both a pressure-driven flow and/or an electroosmotic flow in the fluid.
This application note explains how a droplet manipulation setup using embedded conventional indium electrodes could be replaced with a microchip using Electrowell-generated liquid electrodes, with a simpler design that provides enhanced performance.
Many microfluidic applications require switching between multiple solutions (such as samples or buffers) while maintaining a constant flow-rate during the course of their experiment. In this application, one MFCS™-EZ pressure channel is connected via a manifold to ten separate vials containing different aqueous solutions. […]
The use of water-in-oil droplets in microfluidics in high-throughput screening is rapidly gaining acceptance. The main application areas currently involve screening cells as well as genetic material for various mutations or activity. Here the aim is to isolate single DNA molecules and analyze the enzymes and proteins resulting from their expression. […]
Many microfluidic applications require expensive solutions to be injected at a controlled flow-rate into a microfluidic system, such as cell cultures, PCR processes, cell injections or simulation of blood capillaries with a controlled minimal mechanical stress. […]