Automated rare cells capture and labelling
Circulating Tumor Cells (CTCs) are tumoral cells issued from a primary tumor. They transiently circulate in the blood stream until they stop in specific organs to seed metastases. Metastases are involved in most cancer deaths and often develop characteristics which make them resistant to treatments that were efficient on the primary tumors. In this context, being able to study these CTCs represents several assets such as to monitor over time the disease progression as well as to develop the best-suited therapeutic strategies.
Several methods have been studied to capture and detect CTCs. They usually include a large number of steps (injection of several different samples, buffers, etc) and can last several hours.
This application note describes the automation of a 5-hour CTC capture and detection method including the precise volume control of more than 10 different solutions.
The use of water-in-oil droplets in microfluidics in high-throughput screening is rapidly gaining acceptance. The main application areas currently involve screening cells as well as genetic material for various mutations or activity. Here the aim is to isolate single DNA molecules and analyze the enzymes and proteins resulting from their expression. […]
Many microfluidic applications are developed based on concepts relying on electrodes, exploiting the electrical properties of samples to sort or separate them, generate electro-osmotic flows, manipulate particles, perform electrochemical detection etc. One example of this is electronic paper, where the electrophoretic displacement of titanium dioxide particles in a dark hydrocarbon oil solution allows the formation […]
Information, such as cell size, type discrimination and other quantitative characteristic information can be measured for biological systems using impedance spectroscopy. Our electrical impedance spectroscopy platform (or EISP) offers the advantage of acquiring this information from cells, or particles such as beads, without the need to label them, as would be needed for characterization using […]