Biomillenia’s microbiome-on-a-chip technology uses droplet generation to create bacterial cell banks, evaluate microorganisms for desired phenotypes and to find new routes to prevent and treat dysbiosis. Most of the microfluidic droplet techniques require biocompatible surfactants to keep the droplets stable but it can be affected by growth of bacteria in droplets. Hence, choosing the optimal surfactant for long-term incubation while being biocompatible for a wide spectrum of bacterial species is essential.
Here, the performance of three commonly used surfactants are compared at three different concentrations. The droplet stability over time and the droplet occupation rate is determined by encapsulating a microbial community derived from human skin.